Distinguishing iron deficiency from beta‐thalassemia trait by new generation ektacytometry

Krishnevskaya, Elena; Payán-Pernía, Salvador; Hernández‐Rodríguez, Inés; Sevilla, A.; Serra, Águeda Ancochea; Morales‐Indiano, Cristian; Ferrer, Montserrat Bosch; Vives‐Corrons, Joan‐Lluis

doi:10.1111/ijlh.13362

Cited by 7 publications

(11 citation statements)

References 7 publications

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“…Figure 11 shows the Osmoscan curve for the healthy subjects, IDA samples and β-TT samples. These results were previously described and differences between the three groups were statistically significant, reflecting deformability differences between these three states ( Payán-Pernía et al, 2021 ). When β-TT is present, the ektacytometry curve is displaced to the left with respect to healthy RBC, revealing a decrease in the RBC deformability capacity.…”

Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

“…The higher viscosity obtained for the β-TT samples might be attributed to a greater rigidity in the RBC membrane. This can be caused by the excess of α chains that bind to the RBC membrane (DeLoughery, 2017;Payán-Pernía et al, 2021). To verify that the differences in viscosity found between β-TT, IDA, and healthy donors are caused by changes in the properties of RBCs, we compare the results obtained with the LoRRCA ektacytometer between the samples.…”

Section: Comparison Of Blood Samples With Microcytic Red Blood Cells: Iron Deficiency Anemia and Beta-thalassemia Traitmentioning

confidence: 99%

“…Previous works suggest that the decreased RBC deformability is probably due to microcytosis ( Rasia et al, 1986 ; Iborra et al, 2003 ). More recently, by comparing two non-healthy sets of samples, of iron deficiency anemia (IDA) ( DeLoughery, 2017 ) and β-thalassemia trait, a study based on ektacytometry showed two distinct deformation patterns, indicating that the observed decrease in the deformability is not only due to microcytosis ( Payán-Pernía et al, 2021 ). Osmotic gradient ektacytometry measures RBC deformability under defined shear stress as a function of suspending medium osmolarity ( Llaudet-Planas et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

et al. 2021

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The purpose of this work is to develop a hematocrit-independent method for the detection of beta-thalassemia trait (β-TT) and iron deficiency anemia (IDA), through the rheological characterization of whole blood samples from different donors. The results obtained herein are the basis for the development of a front microrheometry point-of-care device for the diagnosis and clinical follow-up of β-TT patients suffering hematological diseases and alterations in the morphology of the red blood cell (RBC). The viscosity is calculated as a function of the mean front velocity by detecting the sample fluid-air interface advancing through a microfluidic channel. Different viscosity curves are obtained for healthy donors, β-TT and IDA samples. A mathematical model is introduced to compare samples of distinct hematocrit, classifying the viscosity curve patterns with respect to the health condition of blood. The viscosity of the fluid at certain shear rate values varies depending on several RBC factors such as shape and size, hemoglobin (Hb) content, membrane rigidity and hematocrit concentration. Blood and plasma from healthy donors are used as reference. To validate their potential clinical value as a diagnostic tool, the viscosity results are compared to those obtained by the gold-standard method for RBC deformability evaluation, the Laser-Optical Rotational Red Cell Analyzer (LoRRCA).

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Section: Comparison Of Blood Samples With Microcytic Red Blood Cells: Iron Deficiency Anemia and Beta-thalassemia Traitmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

et al. 2021

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“…Although there is a deterministic relationship between hydration state and cell morphology in healthy RBCs as indicated by the SDE-scale (Lim et al, 2002 ), in the pathophysiological situations this relationship is widely distorted as the dehydration due to hemoglobinopathies indicate (Zaninoni et al, 2018 ; Krishnevskaya et al, 2021 ). Therefore, we believe the hydration state of the RBCs is an unsuitable parameter for disease classification.…”

Section: Dehydrated Stomatocytosis Is a Terminology Contradiction In Itselfmentioning

confidence: 99%

Rare Anemias: Are Their Names Just Smoke and Mirrors?

et al. 2021

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Altered blood rheology in multiwidth microchannels: Hematocrit and tonicity variation

Riera-Llobet,

Méndez-Mora,

Cabello-Fusarés

et al. 2023

Physics of Fluids

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We present a theoretical model for the characterization of the behavior of Newtonian and non-Newtonian fluids inside a microchannel with segments of different widths. This allows us to find the relation required to have collapsed viscosity curves for different fluids in just one experiment. Diverse experiments have been carried out with our setup. We consider different hematocrit concentration samples as well as samples of blood altered with different solutions in order to modify their tonicity. The data are acquired using microscale electronic detection of a fluid/air front advance. This will disclose different diseases that can be distinguished by the change in the normal rheological characteristics of blood or plasma. The results obtained demonstrate that using our setup and mathematical model, we can both distinguish blood with different concentrations of erythrocytes as well as discriminate when a same sample of blood is non-altered, altered with sodium chloride (NaCl) or de-ionized water (DIW), which emulates the abnormalities in the red blood cells of some pathologies. The results show that DIW causes blood to have more non-Newtonian behavior. On the other hand, NaCl causes the sample to become a hypertonic solution, causing the red blood cells to crenate, causing the blood to have a more Newtonian behavior.

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Distinguishing iron deficiency from beta‐thalassemia trait by new generation ektacytometry

Cited by 7 publications

References 7 publications

Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

Blood Rheological Characterization of β-Thalassemia Trait and Iron Deficiency Anemia Using Front Microrheometry

Rare Anemias: Are Their Names Just Smoke and Mirrors?

Altered blood rheology in multiwidth microchannels: Hematocrit and tonicity variation

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