Distinguishing chemically induced NADPH- and NADH-related metabolic responses using phasor analysis of UV-excited autofluorescence

Short, Audrey; Aayedi, Nazar Al; Gaire, Madhu; Kreider, Max; Wong, Chong Kai; Urayama, Paul

doi:10.1039/d1ra02648h

Cited by 2 publications

(3 citation statements)

References 54 publications

(95 reference statements)

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“…20 Furthermore, endogenous molecules, such as glucose and oxygen, are ubiquitous; thus, systemic generation of H 2 O 2 may still occur to induce side effects. 21,22 Cancer-specific H 2 O 2 generation followed by the Fenton reaction can be considered an advantageous strategy for safer and more efficient CDT. Therefore, we suggest a Fe-containing metal-organic framework (MOF), that is, the NH 2 -MIL-101(Fe), to be loaded with D-penicillamine (D-pen) for CDT (Scheme 1).…”

Section: Nox Catalyzed the Conversion Of Endogenous Oxygen Into Omentioning

confidence: 99%

“…Although showing the improved efficacy of CDT with extra H 2 O 2 production, the enzymes of natural proteins still pose challenges, such as immunogenicity, low stability, and high cost 20 . Furthermore, endogenous molecules, such as glucose and oxygen, are ubiquitous; thus, systemic generation of H 2 O 2 may still occur to induce side effects 21,22 …”

Section: Introductionmentioning

confidence: 99%

“… 20 Furthermore, endogenous molecules, such as glucose and oxygen, are ubiquitous; thus, systemic generation of H 2 O 2 may still occur to induce side effects. 21 , 22 …”

Section: Introductionmentioning

confidence: 99%

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Fe‐containing metal–organic framework with D‐penicillamine for cancer‐specific hydrogen peroxide generation and enhanced chemodynamic therapy

Kim

Min

et al. 2023

Bioengineering & Transla Med

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Chemodynamic therapy (CDT) is based on the production of cytotoxic reactive oxygen species, such as hydroxyl radicals (•OH). Thus, CDT can be advantageous when it is cancer‐specific, in terms of efficacy and safety. Therefore, we propose NH2‐MIL‐101(Fe), a Fe‐containing metal–organic framework (MOF), as a carrier of Cu (copper)‐chelating agent, d‐penicillamine (d‐pen; i.e., the NH2‐MIL‐101(Fe)/d‐pen), as well as a catalyst with Fe‐metal clusters for Fenton reaction. NH2‐MIL‐101(Fe)/d‐pen in the form of nanoparticles was efficiently taken into cancer cells and released d‐pen in a sustained manner. The released d‐pen chelated Cu that is highly expressed in cancer environments and this produces extra H2O2, which is then decomposed by Fe in NH2‐MIL‐101(Fe) to generate •OH. Therefore, the cytotoxicity of NH2‐MIL‐101(Fe)/d‐pen was observed in cancer cells, not in normal cells. We also suggest a formulation of NH2‐MIL‐101(Fe)/d‐pen combined with NH2‐MIL‐101(Fe) loaded with the chemotherapeutic drug, irinotecan (CPT‐11; NH2‐MIL‐101(Fe)/CPT‐11). When intratumorally injected into tumor‐bearing mice in vivo, this combined formulation exhibited the most prominent anticancer effects among all tested formulations, owing to the synergistic effect of CDT and chemotherapy.

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Section: Nox Catalyzed the Conversion Of Endogenous Oxygen Into Omentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fe‐containing metal–organic framework with D‐penicillamine for cancer‐specific hydrogen peroxide generation and enhanced chemodynamic therapy

Kim

Min

et al. 2023

Bioengineering & Transla Med

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Cellular NADH and NADPH Conformation as a Real-Time Fluorescence-Based Metabolic Indicator under Pressurized Conditions

et al. 2021

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Cellular conformation of reduced pyridine nucleotides NADH and NADPH sensed using autofluorescence spectroscopy is presented as a real-time metabolic indicator under pressurized conditions. The approach provides information on the role of pressure in energy metabolism and antioxidant defense with applications in agriculture and food technologies. Here, we use spectral phasor analysis on UV-excited autofluorescence from Saccharomyces cerevisiae (baker’s yeast) to assess the involvement of one or multiple NADH- or NADPH-linked pathways based on the presence of two-component spectral behavior during a metabolic response. To demonstrate metabolic monitoring under pressure, we first present the autofluorescence response to cyanide (a respiratory inhibitor) at 32 MPa. Although ambient and high-pressure responses remain similar, pressure itself also induces a response that is consistent with a change in cellular redox state and ROS production. Next, as an example of an autofluorescence response altered by pressurization, we investigate the response to ethanol at ambient, 12 MPa, and 30 MPa pressure. Ethanol (another respiratory inhibitor) and cyanide induce similar responses at ambient pressure. The onset of non-two-component spectral behavior upon pressurization suggests a change in the mechanism of ethanol action. Overall, results point to new avenues of investigation in piezophysiology by providing a way of visualizing metabolism and mitochondrial function under pressurized conditions.

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Distinguishing chemically induced NADPH- and NADH-related metabolic responses using phasor analysis of UV-excited autofluorescence

Abstract: A phasor approach was used to show that chemically induced cellular autofluorescence responses linked to NADPH and NADH pathways can be distinguished.

Cited by 2 publications

References 54 publications

Fe‐containing metal–organic framework with D‐penicillamine for cancer‐specific hydrogen peroxide generation and enhanced chemodynamic therapy

Fe‐containing metal–organic framework with D‐penicillamine for cancer‐specific hydrogen peroxide generation and enhanced chemodynamic therapy

Cellular NADH and NADPH Conformation as a Real-Time Fluorescence-Based Metabolic Indicator under Pressurized Conditions

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