Distinguishing cancer cells from normal cells with an organelle-targeted fluorescent marker

Abstract

This paper reported a hemicyanine dye was used for distinguish cancer cells from normal cells by their different organelles targetability. Probe 1, a red emission hemicyanine functional dye, was connected...

Fluorescence Enhancement of Adamantane‐Modified Dyes in Aqueous Solution via Supramolecular Interaction with Methyl‐β‐cyclodextrin and Their Application in Cell Imaging

“…Thus, three bromine-containing fluorophores including 7bromo-2H-chromen-2-one, 6-bromo-2-methyl-1Hbenzo[de]isoquinoline-1,3(2H)-dione and 9-bromo-5Hbenzo[a]phenoxazin-5-one were grafted with 1-adamantanyl methanamine via nucleophilic aromatic substitution reaction under palladium-catalyzed and then dyes Coum-Ad, NP-Ad, NR-Ad were obtained with isolated yields of 11.9 %, 42.0 % and 21.7 %, respectively. The characterization (containing 1 H NMR, 13 C NMR, FTIR and HRMS(ESI + )) of Coum-Ad, NP-Ad and NR-Ad were recorded in the Supporting Information for details.…”

“…For example, previous study reported a hemicyanine dye that could complex with β‐CD in aqueous solution. However, when the fluorescence quantum yield of this dye in aqueous solution was increased from 1.57 % to 13.1 %, the amount of β‐CD was over 700 times than that of the dye due to low inclusion equilibrium constant (398 M −1 ) [13] . The inclusion equilibrium constants of fluorophores and CDs generally ranged from 10 M −1 to 10 3 M −1 (Table S1).…”

“…However, when the fluorescence quantum yield of this dye in aqueous solution was increased from 1.57 % to 13.1 %, the amount of β-CD was over 700 times than that of the dye due to low inclusion equilibrium constant (398 M À 1 ). [13] The inclusion equilibrium constants of fluorophores and CDs generally ranged from 10 M À 1 to 10 3 M À 1 (Table S1). It may be the reason for low inclusion equilibrium constants that hostguest chemistry can't be generally applied to the field of fluorescence enhancement in aqueous solution.…”

Solid-state emitting twisted π-conjugate as AIE-active DSE-gen:in vitroanticancer properties against FaDu and 4T1 with biocompatibility and bioimaging

“…18–21 Nevertheless, many admirable anticancer drugs are available, but with no bioimaging ability, 22,23 while numerous bioimaging agents are known without anticancer properties. 24 Hence, the discovery of an anticancer agent with bioimaging ability is a looming challenge but a surging need of the time. 25…”

Improved emission performance of benzo[a]phenoxazine in aqueous solution through host–guest interaction

“…8 A low host–guest complexation constant leads to an increase in the demand for β-CDs in the application of biological imaging (>800 eq. ), 9 which may have an abnormal impact on the cellular microenvironment. 10 The structural similarity between adamantane (Ad) and the hydrophobic cavity of β-CDs enables the formation of host–guest complexes with a binding constant in the range of 10 3 –10 4 M −1 at a 1 : 1 stoichiometry, which greatly improves the thorny problem of excessive β-CD dosage.…”