“…However, roots were washed gently with deionized water in this study, and it has been reported that only the application of dithionite-citrate-bicarbonate (DCB) thoroughly removes iron plaques ( McLaughlin et al , 1985 ). One previous report showed that, for root surfaces without iron plaques, Fe was difficult to detect by SEM-EDX at the surface, although the total Fe content (DCB-extracted) was high in roots ( Mi et al , 2013 ), similar to the present SEM-EDX result ( Supplementary Fig. S6A ).…”