Distinctive roles between rumen epimural and content bacterial communities on beef cattle feed efficiency: A combined analysis

Zhou, Mi; Ghoshal, Bibaswan; Stothard, Paul; Guan, Le Luo

doi:10.1016/j.crmicr.2021.100085

Cited by 8 publications

(14 citation statements)

References 44 publications

(62 reference statements)

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“…Without examining each rumen sac, valuable data may be overlooked. Research may consider examining these communities due to the importance of the epimural community in maintaining ruminal homeostasis and host-microbe interactions (Zhou et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Verrucomicrobiota have been examined in a lignocellulosic forage studies, where bacteria in this phylum have carbohydrate active enzymes, sulfatases, and peptidases (Gharechahi et al, 2022) which facilitate carbohydrate polysaccharide metabolism and may aid in lignocellulose breakdown within the rumen. In general, having a major phylum in the planktonic community being involved in cellulose degradation is not uncommon (Schären et al, 2017;Tan et al, 2021;Zhou et al, 2021), but it is more unexpected for the epimural community where Proteobacteria are typically detected at greater abundances (De Mulder et al, 2016;Sbardellati et al, 2020;Pacifico et al, 2021;Zhou et al, 2021). Genera within Proteobacteria are comprised of aerobes and facultative anaerobes, and thus are able to participate in oxygen scavenging (De Mulder et al, 2016;Zhou et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Rumen biogeographical regions and their impact on microbial and metabolome variation

Soltis¹,

Henniger²,

Egert-McLean³

et al. 2023

Front. Anim. Sci.

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The rumen microbiome is a complex microbial network critical to the health and nutrition of its host, due to their inherent ability to convert low-quality feedstuffs into energy. In rumen microbiome studies, samples from the ventral sac are most often collected because of the ease of access and repeatability. However, anatomical musculature demarcates the rumen into five sacs (biogeographical regions), which may support distinct microbial communities. The distinction among the microbes may generate functional variation among the rumen microbiome, thus, specialized tasks within different sacs. The objective of this study was to determine the rumen liquid metabolome and epimural, planktonic, and fiber-adherent bacterial communities among each rumen biogeographical region. It was hypothesized that differences in bacterial species and metabolome would occur due to differing anatomy and physiology associated with the respective regions. To assess this variation, epithelial and content microbial-associated communities were evaluated, as well as the metabolites among various rumen biogeographical regions. A total of 17 cannulated Angus cows were utilized to examine the fiber-adherent (solid fraction), planktonic (liquid fraction), and epimural microbial communities from the cranial, dorsal, caudodorsal blind, caudoventral blind, and ventral sacs. Metagenomic DNA was extracted and sequenced from the hypervariable V4 region of the 16S rRNA gene. Reads were processed using packages ‘phyloseq’ and ‘dada2’ in R. Untargeted metabolomics were conducted on rumen liquid from each sac using UHPLC-HRMS and analyzed in MetaboAnalyst 5.0. An analysis of variance (ANOVA) revealed 13 significant differentially abundant metabolites with pairwise comparisons against the five rumen sacs (P < 0.05). Within the bacterial communities, neither alpha nor beta diversity determined significance against the rumen sacs (P > 0.05), although there was significance against the fraction types (P < 0.05). Utilizing multivariable association analysis with MaAslin2, there were significant differential abundances found in fraction type × location (P < 0.05). Knowledge of similarities among fiber-adherent microbial communities provides evidence that single sac sampling is sufficient for this fraction. However, future projects focusing on either planktonic or epimural fractions may need to consider multiple rumen sac sampling to obtain the most comprehensive analysis of the rumen. Defining these variabilities, especially among the rumen epimural microbiome, are critical to define host-microbiome interactions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rumen biogeographical regions and their impact on microbial and metabolome variation

Soltis¹,

Henniger²,

Egert-McLean³

et al. 2023

Front. Anim. Sci.

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“…Besides, the provision of more easily degradable proteins helps increase the relative abundances of Firmicutes and Proteobacteria under the high-concentrate diet feeding process (26,27). These changes may trigger the active degradability of high-fermentable carbohydrates and lead to the increment of carbohydrate degradation into VFAs.…”

Section: E Ects Of Small Peptides On Rumen Carbohydrate Fermentabilitymentioning

confidence: 99%

Rumen microbiota responses to the enzymatic hydrolyzed cottonseed peptide supplement under high-concentrate diet feeding process

Hong²,

Liu³

et al. 2022

Front. Vet. Sci.

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In current dairy production, dietary energy is always excessively provided with a high-concentrate diet feeding to improve milk production. However, this feeding practice disturbed the rumen microbial ecosystem and the balance between ruminal energy and nitrogen, resulting in decreased nutrient fermentability, which in turn declined the milk yield of dairy cows. Therefore, supplementation of dietary degradable nitrogen may be helpful for high dairy production. In this study, we evaluated the regulatory effects of easily utilized enzymatic hydrolyzed cottonseed peptide (EHP) supplements on rumen microbiota communities and rumen nutrient fermentability under high-concentrate feeding. For this purpose, a gradient concentrate of EHP (from 0.2 to 1.0%) was added to the high-concentrate basal substrates for an in vitro experiment. Each treatment contained three replicates, with three bottles in each replicate. Rumen fermentable parameters included microbial protein content, volatile fatty acids, and ammonia-N; the rumen nutrient degradability of dry matter, crude protein, neutral detergent fiber, acid detergent fiber, ether extracts, calcium, and phosphorus were further investigated after in vitro fermentation for 72 h. Then, rumen microbiota communities and their correlation with ruminal fermentation parameters and rumen nutritional degradability were analyzed to understand the regulatory mechanism of the EHP supplements on rumen fermentability. Results indicate that treatment with 0.6% of EHP supplements had the highest content of acetate, butyrate, and neutral detergent fiber degradability among all treatments. Furthermore, EHP supplements significantly increased the relative abundance of rumen cellulose and starch-degrading bacteria such as Ruminococcus, Bifidobacterium, and Acetitomaculum, and the high nitrogen utilizing bacteria Butyrivibrio and Pseudobutyrivibrio, which may further promote the rumen carbohydrate and nitrogen metabolism. In summary, supplementation of easily degraded small peptides helps reestablish rumen energy and nitrogen balance to promote the rumen fermentable functions and nutritional degradability under high-concentrate diet feeding circumstances. These findings may further promote dairy production.

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“…Rumen microbial communities are essential to host health and performance as microbial fermentation of feed provides key metabolic products for the host animal ( Russell and Hespell, 1981 ; Bergman, 1990 ; Hess et al, 2011 ; Morgavi et al, 2013 ; Weimer, 2015 ; Paz et al, 2018 ; Xue et al, 2020 ; Zhou et al, 2021 ). Rumen microorganisms produce nutritionally important fermentation products such as short chain fatty acids (SCFA) and vitamins via the breakdown of cellulose, hemicellulose, pectin, and other ingested feed ( Bergman, 1990 ; Seck et al, 2017 ; Averianova et al, 2020 ; Beckett et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Rumen microorganisms have been characterized into three main groups: rumen solid-associated microorganisms, rumen fluid microorganisms, and rumen epithelial microbiota. The first two groups are collectively denoted as rumen content microbiota ( McCowan et al, 1978 ; Cheng et al, 1979 ; Zhou et al, 2021 ; Na and Guan, 2022 ). Several studies have analyzed the community structure and function of the rumen content microbiota, emphasizing its importance in feed efficiency and host performance ( Hackmann and Spain, 2010 ; Henderson et al, 2015 ; Gruninger et al, 2019 ; O’Hara et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Influence of a sodium-saccharin sweetener on the rumen content and rumen epithelium microbiota in dairy cattle during heat stress

Koester

Hayman

Anderson

et al. 2022

Journal of Animal Science

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The effect of a saccharin-based artificial sweetener was tested on animal performance measures and on the microbial communities associated with the rumen content and with the rumen epithelium during heat stress. Ten cannulated Holstein-Friesian milking dairy cattle were supplemented with 2 grams of saccharin-based sweetener per day, top-dressed into individual feeders for a 7-day adaptation period followed by a 14-day heat stress period. A control group of ten additional cows subjected to the same environmental conditions but not supplemented with sweetener were included for comparison. 16S rRNA gene amplicon sequencing was performed on rumen content and rumen epithelium samples from all animals, and comparisons of rumen content microbiota and rumen epithelial microbiota were made between supplemented and control populations. Supplementation of the saccharin-based sweetener did not affect the rumen content microbiota, but differences in the rumen epithelial microbiota beta-diversity (PERMANOVA, P = 0.003, R 2 = 0.12) and alpha-diversity (Chao species richness, P = 0.06 and Shannon diversity, P = 0.034) were detected between the supplemented and control experimental groups. Despite the changes detected in the microbial community, animal performance metrics including feed intake, milk yield, and short-chain fatty acid (acetic, propionic, and butyric acid) concentrations were not different between experimental groups. Thus, under the conditions applied, supplementation with a saccharin-based sweetener does not appear to affect animal performance under heat stress. Additionally, we detected differences in the rumen epithelial microbiota due to heat stress when comparing initial, pre-stressed microbial communities to the communities after heat stress. Importantly, the changes occurring in the rumen epithelial microbiota may have implications on barrier integrity, oxygen scavenging, and urease activity. This research adds insight into the impact of saccharin-based sweeteners on the rumen microbiota and the responsivity of the rumen epithelial microbiota to different stimuli, providing novel hypotheses for future research.

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Distinctive roles between rumen epimural and content bacterial communities on beef cattle feed efficiency: A combined analysis

Cited by 8 publications

References 44 publications

Rumen biogeographical regions and their impact on microbial and metabolome variation

Rumen biogeographical regions and their impact on microbial and metabolome variation

Rumen microbiota responses to the enzymatic hydrolyzed cottonseed peptide supplement under high-concentrate diet feeding process

Influence of a sodium-saccharin sweetener on the rumen content and rumen epithelium microbiota in dairy cattle during heat stress

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