Distinctive phenotypic and molecular characteristics of Brucella abortusstrains isolated from Mongolia

Kim, Jiyeon; Kang, Sung-Il; Erdenebaataar, Janchivdorj; Vanaabaatar, Batbaatar; Ulziisaikhan, Gombosuren; Khursbaatar, Ochirbat; Lee, Kichan; Lee, Jin Ju; Sung, So-Ra; Jung, Suk-Chan; Park, Taesung; Yoo, Heejin; Her, Moon

doi:10.3906/vet-1511-75

Cited by 4 publications

(3 citation statements)

References 21 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Readers should note that the original biovar 7 reference strain (63/75) has been shown to represent a mixed culture and biovar 7 was thus deleted from Brucella taxonomy (Corbel, 1988; Garin-Bastuji et al, 2014). However genetically diverse isolates that possess the phenotypic profile reported for biovar 7 have been included here and reported in other studies (Garin-Bastuji et al, 2014; Kim et al, 2016). The genetic diversity of strains with the biovar 7 phenotype questions any move to reintroduce biovar 7 as previously defined.…”

Section: Resultsmentioning

confidence: 99%

Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars

et al. 2016

View full text Add to dashboard Cite

An extended multilocus sequence analysis (MLSA) scheme applicable to the Brucella, an expanding genus that includes zoonotic pathogens that severely impact animal and human health across large parts of the globe, was developed. The scheme, which extends a previously described nine locus scheme by examining sequences at 21 independent genetic loci in order to increase discriminatory power, was applied to a globally and temporally diverse collection of over 500 isolates representing all 12 known Brucella species providing an expanded and detailed understanding of the population genetic structure of the group. Over 100 sequence types (STs) were identified and analysis of data provided insights into both the global evolutionary history of the genus, suggesting that early emerging Brucella abortus lineages might be confined to Africa while some later lineages have spread worldwide, and further evidence of the existence of lineages with restricted host or geographical ranges. The relationship between biovar, long used as a crude epidemiological marker, and genotype was also examined and showed decreasing congruence in the order Brucella suis > B. abortus > Brucella melitensis. Both the previously described nine locus scheme and the extended 21 locus scheme have been made available at http://pubmlst.org/brucella/ to allow the community to interrogate existing data and compare with newly generated data.

show abstract

Section: Resultsmentioning

confidence: 99%

Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars

et al. 2016

View full text Add to dashboard Cite

show abstract

“…This finding is consistent with the screening in all Mongolian provinces with a correlation of camel and cattle seropositivity at district level, as well as previous reports of identification on Brucella spp. from camels in Asia, where another isolate from a Mongolian camel also was B. abortus [37]. Monitoring surveys of achieved vaccination coverage from 2012 to 2015 indicate that sufficient coverage was achieved in small ruminants, but coverage was critically low in cattle.…”

Section: Discussionmentioning

confidence: 99%

Risk factors of brucellosis seropositivity in Bactrian camels of Mongolia

Bayasgalan

Chultemdorj

Roth³

et al. 2018

BMC Vet Res

View full text Add to dashboard Cite

BackgroundMore information on brucellosis epidemiology in Bactrian camels is needed due to their growing economic and livelihood importance for herders and renewed efforts in Mongolia to eliminate brucellosis through mass vaccination of ruminants excluding camels. Brucellosis prevalence in camels increased over the past two decades. Random multi-stage cluster surveys were done in the Eastern provinces of Dornod and Sukhbaatar in 2013 and 2014 and in the Southern & Western provinces of Dornogobi, Umnogobi and Khovd in 2014 and 2015. A total of 1822 camels, 1155 cattle, and 3023 small ruminant sera were collected and tested with the Rose Bengal Test. In addition, 195 vaginal swabs and 250 milk samples for bacteriological culture were taken from livestock with history of abortion.ResultsThe overall apparent seroprevalence in camels was 2.3% (95% confidence interval 1.6–3.3). The main risk factor for camel seropositivity was being in an Eastern province when compared to Southern & Western provinces (odds ratio 13.2, 95% CI 5.3–32.4). Camel seroprevalences were stable over the two consecutive survey years, despite introduction of ruminant vaccination: 5.7% (95% CI 3.1–10.2%) and 5.8% (3.3–10.1%) in Eastern provinces and 0.4% (0.2–1.2%) and 0.5% (0.1–2.0%) in Southern & Western provinces. We isolated Brucella abortus from camels and cattle. Camel seropositivity was associated to keeping cattle together with camels. Monitoring of vaccination campaigns showed that coverage in cattle was insufficient because animals could not be adequately restrained.ConclusionsThe present study reveals that brucellosis is present with important seroprevalence in Mongolian camels and was endemic in Eastern provinces. Camel herd seropositivity was most closely associated to infection in cattle.Longer term monitoring is needed to assess whether camel seroprevalance decreases with ongoing vaccination in Mongolia. This should be coupled with further confirmation on Brucella spp. isolates. To date, only Brucella abortus was isolated, but camels are also susceptible to Brucella melitensis. Clear verbal and written information on disease prevention in livestock and household members is important, particularly for remote camel herders who had only moderate knowledge on brucellosis epidemiology and preventive measures.Electronic supplementary materialThe online version of this article (10.1186/s12917-018-1664-0) contains supplementary material, which is available to authorized users.

show abstract

“…Data related to the epidemiology of brucellosis in Bactrian camels have been reported infrequently in the international literature (Bayasgalan et al, 2018;Kim et al, 2016). Both studies were performed in Mongolia and suggested that Bactrian camels are secondary hosts for Brucella spp.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of camel brucellosis and associated risk factors

Dadar

Shahali

Omar

et al. 2022

Qual. Assur. Saf. Crops Foods

View full text Add to dashboard Cite

Camel brucellosis is a widespread and critical zoonotic infection caused by Brucella abortus and Brucella melitensis that negatively impact dairy and meat products. The current meta-analysis covers studies published over a course of 40 years, between 1 January 1980 and 1 April 2021, aiming to investigate the global prevalence of Brucella spp. in camels and related risk factors in different camel-keeping areas. Chi-squared test (I2 index) was applied to evaluate the heterogeneity of studies. A random-effect model (REM) estimated the pooled prevalence among subgroups. The literature search on camel brucellosis was performed among the available databases, including CABI, PubMed, Embase, Web of Science, Cochrane, Scielo, Scopus, and Science Direct. A total of 56 publications, comprising 205 data reports, were evaluated. Our results showed that the overall prevalence of camel brucellosis in the world was 9.23%. The lowest and highest prevalence rates of Brucella spp. infections among camels were reported in Oman (0.34%, 95%CI: 0.18–0.55) and Sudan (37.41%, 95%CI: 25.27–50.31), respectively. According to different risk factors, subgroup analysis showed that the location, sex, herd size, age, and mixed rearing with other animals could be considered the main risk factors impacting the occurrence of brucellosis in camels. The reported Brucella spp. in dromedary camel was Brucella melitensis (Biovar 1, 2, and 3), Brucella abortus (Biovar 3, 5, and 6), and Brucella suis. Our study represented remarkable differences between the prevalence of Brucella spp. in male (6.83% in 41 studies) and female (9.64% among 62 studies) camels. However, the present meta-epidemiological study would help improve control and surveillance approaches to prevent the spread of camel brucellosis among herds.

show abstract

Distinctive phenotypic and molecular characteristics of Brucella abortusstrains isolated from Mongolia

Cited by 4 publications

References 21 publications

Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars

Extended Multilocus Sequence Analysis to Describe the Global Population Structure of the Genus Brucella: Phylogeography and Relationship to Biovars

Risk factors of brucellosis seropositivity in Bactrian camels of Mongolia

The prevalence of camel brucellosis and associated risk factors

Contact Info

Product

Resources

About