Distinctive MicroRNA Profiles Relating to Patient Survival in Esophageal Squamous Cell Carcinoma

Guo, Yiming; Chen, Zhaoli; Liang, Zhen; Zhou, Fang; Shi, Susheng; Feng, Xiaoli; Li, Baozhong; Meng, Xiangjiao; Ma, Xi; Luo, Mingyong; Shao, Kwang‐Tsao; Li, Ning; Qiu, Bin; Mitchelson, Keith; Cheng, Jing; He, Jié

doi:10.1158/0008-5472.can-06-4418

Cited by 313 publications

(281 citation statements)

References 48 publications

(52 reference statements)

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“…17 Several studies have showed that miRNAs might contribute to human carcinogenesis and cancer progression and be considered as potential novel targets for cancer diagnosis and treatment. 2,8 The global miRNA expression profile in ESCC was demonstrated for the first time in 2008, 18 and some miRNAs have been reported to exert regulatory functions in ESCC. miRNAs, including miR-21, miR-31, miR-577, etc, act as oncogenes, [19][20][21] while others such as Let-7, miR-98, miR-100, miR-138, miR-143, miR-150, etc, function as tumor suppressors.…”

Section: Discussionmentioning

confidence: 99%

microRNA-186 inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma by targeting SKP2

Jiao

Zhang

et al. 2016

Laboratory Investigation

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miR-186 has been demonstrated to have a significant role as a tumor suppressor in many types of cancers. Nevertheless, its biological function in esophageal squamous cell carcinoma (ESCC) remains unknown. In the present study, we found that the expression level of miR-186 was downregulated in ESCC in comparison with the adjacent normal tissues and was significantly associated with differentiation level, TNM stage, and lymph node metastasis of ESCC. Functional experiments revealed that enforced overexpression of miR-186 in ESCC cells suppressed the proliferation, invasion, and induced the apoptosis of cells. Luciferase reporter assay and western blotting analysis were performed to verify the target gene regulated by miR-186, SKP2. Our findings established that the miR-186 has a suppressive role in ESCC progression via SKP2-mediated pathway, and this implies that miR-186 could be a potential therapeutic target for ESCC.

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Section: Discussionmentioning

confidence: 99%

microRNA-186 inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma by targeting SKP2

Jiao

Zhang

et al. 2016

Laboratory Investigation

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“…Procedures were performed as described in Guo et al (2008). microRNAs were isolated from the breast cancer cell lines as well as breast cancer tissues using the miRNeasy Mini Kit (Qiagen, Valencia, CA, USA), according to the manufacturer's protocol.…”

Section: Methodsmentioning

confidence: 99%

Downregulation of miR-193b contributes to enhance urokinase-type plasminogen activator (uPA) expression and tumor progression and invasion in human breast cancer

2009

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Emerging evidence suggests the potential involvement of altered regulation of miRNAs in the pathogenesis of cancers, and these miRNAs are thought to be functional as tumor suppressors or oncogenes. Using miRNA arrays, we identified an miRNA differentially expressed between the MDA-MB-231 cell line and its highly metastatic variant. A bioinformatics search revealed a potential target site for miR-193b within the 3 0 UTR of uPA. Ectopic expression of miR-193b repressed the expression of sensor constructs harboring the 3 0 UTR of uPA in breast cancer cell lines. Anti-miR-193b treatment led to an increase of uPA protein and increased cell invasion in MDA-MB-231 cells. In contrast, overexpression of miR193b significantly reduced uPA protein amounts and inhibited cell invasion in MDA-MB-231 and MDA-MB-435 cells. In an immunodeficient mouse model, miR-193b significantly inhibited the growth and dissemination of xenograft tumors. Immunohistochemical staining and real-time PCR assays showed that miR-193b was a negative regulator of the uPA gene in primary breast tumors. Our research reveals that miR-193b is closely associated with clinical metastasis and identifies miR193b potentially targets uPA transcripts. Perturbation of the miRNA-mRNA pairing may have important roles in the initiation and development of breast cancer.

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“…To date, has-miR-335, has-miR-181d, has-miR-25, has-miR-7, and has-miR-495 have been confirmed correlated with histopathological stages of EC. In addition, abnormal miRNA expression spectra are different between esophageal squamous cell carcinoma (ESCC) tissues and esophageal adenocarcinoma (EAC) tissues (Guo et al, 2008;Mathée et al, 2009).…”

Section: Screening Of Microrna In Patients With Esophageal Cancer At mentioning

confidence: 99%

Screening of MicroRNA in Patients with Esophageal Cancer at Same Tumor Node Metastasis Stage with Different Prognoses

Zhao

Liu²,

Tianyun³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Patients at the same pathological stage of esophageal cancer (EC) that received the same surgical therapy by the same surgeon may have distinct prognoses. The current study aimed to explore the possibility of differentiallyexpressed microRNAs (miRNAs) underlying this phenomenon. Samples were collected from EC patients at the same tumor node metastasis (TNM) stage but with different prognoses. Paracancerous normal tissues were taken as controls. The specimens were histopathologically analyzed. Differentially-expressed miRNAs were analyzed using real-time quantitative reverse transcription polymerase chain reaction. Compared with patients with poor prognosis, those with good prognosis exhibited 88 two-fold or more than two-fold increased miRNA fragments and 4 half-decreased miRNAs. The most noticeably up-regulated miRNAs included hsa-miR-31, hsa-miR196b, hsa-miR-652, hsa-miR-125a-5p, hsa-miR-146b, hsa-miR-200c, hsa-miR-23b, hsa-miR-29a, hsa-miR-186, hsa-miR-205, hsa-miR-376a, hsa-miR-410, hsa-miR-532-3p, and hsa-miR-598, whereas the most significantlydownregulated miRNAs were hsa-let-7e, hsa-miR-130b, and hsa-miR-103. EC patients at same TNM stage but with different prognoses show differentially-expressed miRNAs.

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Distinctive MicroRNA Profiles Relating to Patient Survival in Esophageal Squamous Cell Carcinoma

Cited by 313 publications

References 48 publications

microRNA-186 inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma by targeting SKP2

microRNA-186 inhibits cell proliferation and induces apoptosis in human esophageal squamous cell carcinoma by targeting SKP2

Downregulation of miR-193b contributes to enhance urokinase-type plasminogen activator (uPA) expression and tumor progression and invasion in human breast cancer

Screening of MicroRNA in Patients with Esophageal Cancer at Same Tumor Node Metastasis Stage with Different Prognoses

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