Distinctive Immunological Functions of HLA-G

Amodio, Giada; Gregori, Silvia

doi:10.5772/35248

Cited by 4 publications

(8 citation statements)

References 117 publications

(109 reference statements)

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“…Indeed, HLA-G discovery as a tolerogenic molecule in ADSCs with endocrine and paracrine effects would be a therapeutic goal to improve autoimmune diseases, accept solid allografts and treat spontaneous recurrent abortions [ 5 ]. HLA-G has two forms: soluble and membrane-bound with the same immunomodulatory effects in environment [ 7 ]. Overall, membrane-bound HLA-G (mHLA-G) directs inhibitory mechanism through interaction with three well-known inhibitory receptors: ILT2 (Immunoglobulin-Like Transcript), ILT4 and KIR2DL4 (Killer Cell Immunoglobulin-Like Receptor) on monocyte, MQ (macrophage), B, T and NK (Natural Killer) cells and so suppress antigen-specific TCD8 and NK cytotoxic activity.…”

Section: Introductionmentioning

confidence: 99%

“…Many of cytokines, hormones and drugs impress on 5' UTR site. P4 can bind to PRE (Progesterone Response Element) site in the HLA-G promoter between positions -52 and -38 where overlaps the HLA-G TATA box and induce HLA-G expression [ 7 8 9 ] whereas miR-148a, miR-148b and miR-152 can down-regulate HLA-G expression by binding to the 3' UTR HLA-G mRNA [ 10 11 12 13 ].…”

Section: Introductionmentioning

confidence: 99%

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The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells

Moslehi

Hashemibeni

Moslehi

et al. 2016

Korean J Physiol Pharmacol

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Membrane-bound HLA-G (mHLA-G) discovery on adipose derived stem cells (ADSCs) as a tolerogenic and immunosuppressive molecule was very important. Many documents have shown that HLA-G expression can be controlled via some hormones such as progesterone (P4) and estradiol (E2). Therefore, this study was designed to evaluate progesterone and estradiol effects on mHLA-G in ADSCs at restricted and combination concentrations. Three independent cell lines were cultured in complete free phenol red DMEM and subcultured to achieve suffi cient cells. These cells were treated with P4, E2 and P4 plus E2 at physiologic and pregnancy concentrations for 3 days in cell culture conditions. The HLA-G positive ADSCs was measured via monoclonal anti HLA-G-FITC/MEMG-09 by means of flow cytometry in nine groups. Data were analyzed by one way ANOVA and Tukey's post hoc tests. There were no signifi cant values of the mean percentage of HLA-G positive cells in E2-treated and the combination of P4 plus E2-treated ADSCs compared to control cells (p value>0.05) but P4 had a signifi cant increase on mHLA-G in ADSCs (p value<0.05). High P4 concentration increased mHLA-G but E2 and the combination of P4 plus E2 could not change mHLA-G on ADSCs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of progesterone and 17-β estradiol on membrane-bound HLA-G in adipose derived stem cells

Moslehi

Hashemibeni

Moslehi

et al. 2016

Korean J Physiol Pharmacol

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“…Under normal conditions, most cells and tissues show a basal rank of HLA-G transcription. However, translation of the HLA-G transcript occurs only in trophoblasts at the fetal-maternal interface and in cornea, thymic epithelial cells, nail matrix, pancreatic cells, mesenchymal stem cells, erythroid and endothelial precursors in adults (Amodio & Gregori, 2012).…”

Section: Hla-g Transcriptmentioning

confidence: 99%

“…A full-length molecule, HLA-G1, is the lone isoform to be expressed on the cell surface. Membrane-bound HLA-G isoforms and soluble perform comparable functions (Amodio & Gregori, 2012;Klitkou et al, 2015).…”

Section: Hla-g Proteinsmentioning

confidence: 99%

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Role of 14-Bp HLA-G, INDEL Polymorphism in Recurrent Miscarriage

Afkhami¹,

Yazdani²,

Khaniani³

et al. 2016

GJHS

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Mothers and their fetuses are hereditarily unlike. Surprisingly, no less than 50% human pregnancies reach full term despite the tendency of the immune system to eliminate of non-self units. Reduction of adaptive maternal immune answer, which is planned to reject strange factors, is essential for a pregnancy to reach full term. However, approximately 5% couples trying to conceive experience 2 recurrent miscarriages (RMs).HLA-G, which is produced by the external trophectoderm layer and has unique biological features, is involved in the implantation and maintenance of fetus. Serum HLA-G levels are correlated with the risk of RM. Recent studies indicate that a 14-bp HLA-G, INDEL polymorphism decreases the level of HLA-G mRNA, which in turn decreases the amount of HLA-G produced. An understanding of gene parameter and the function of polymorphic sites in the functioning of HLA-G products may enable the development of approaches targeting HLA-G for more detail of causes of RM.

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