Distinctive Feature of Microbial Communities and Bacterial Functional Profiles in Tricholoma matsutake Dominant Soil

Oh, Seung‐Yoon; Fong, Jonathan J.; Park, Myung Soo; Lim, Young Woon

doi:10.1371/journal.pone.0168573

Cited by 37 publications

(58 citation statements)

References 86 publications

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“…It suggested that the dominant bacterial and fungal species could change in the presence of T. matsutake . This observation was supported by previous studies, which found that T. matsutake dominated diverse microbial communities in different forest soils and generates a dominance effect on microbial communities in soil . Kim et al found that Proteobacteria was more abundant than Acidobacteria in the fairy ring zone, which is consistent with our result that Proteobacteria was more abundant than Acidobacteria in tissues of Q. aquifolioides mycorrhized with T. matsutake .…”

Section: Discussionsupporting

confidence: 93%

Ectomycorrhization of Tricholoma matsutake with Quercus aquifolioides affects the endophytic microbial community of host plant

Jin

et al. 2018

J Basic Microbiol

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Tricholoma matsutake (S. Ito et Imai) is an ectomycorrhizal basidiomycete associated with Pinaceae and Fagaceae trees in the Northern Hemisphere. It is still unknown whether the symbiotic relationship with this ectomycorrhiza could affect the host plant's endophytic microbial community. In this study, we used high throughput sequencing to analyze the endophytic microbial communities of different Quercus aquifolioides tissues with or without T. matsutake partner. About 35,000 clean reads were obtained per sample, representing 34 bacterial phyla and 7 fungal phyla. We observed 3980 operational taxonomic units (OTUs) of bacteria and 457 OTUs of fungi at a 97% similarity level. Three bacterial phyla, Proteobacteria, Cyanobacteria, and Bacteroidetes, and the fungal phylum Ascomycota were dominant in all tissues. The relative abundance of these taxa differed significantly between Q. aquifolioides tissues with and without T. matsutake partner (p < 0.05). The bacterial genus Pseudomonas and the fungal genus Cryptosporiopsis were more abundant in mycorrhized roots than in control roots. This study showed that the community structure and dominant species of endophytic microbial communities in Q. aquifolioides tissues might be altered by colonization with T. matsutake. This work provides a new insight into the interactions between ectomycorrhizal fungus and host plant.

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Section: Discussionsupporting

confidence: 93%

Ectomycorrhization of Tricholoma matsutake with Quercus aquifolioides affects the endophytic microbial community of host plant

Jin

et al. 2018

J Basic Microbiol

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“…Increasingly, omics‐based tools are being used to assess the phylogenetic composition of soil microbial communities. One such bioinformatics tool that allows prediction of functional profiles based on phylogenetic composition of communities is PICRUSt (Langille et al, 2013; Oh et al, 2016; Jiménez et al, 2014; Lopes et al, 2016). The objective of this study was to use this tool to examine the predicted functional capabilities of the soil bacterial community under two different tillage systems, long‐term plow tillage and long‐term no‐till.…”

mentioning

confidence: 99%

Functional Predictions of Microbial Communities in Soil as Affected by Long‐term Tillage Practices

Hariharan

Sengupta

Grewal

et al. 2017

Agricultural & Env Letters

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Core Ideas Microbial function is important but difficult to assess in soil. An omics‐driven tool, PICRUSt, was used to characterize functions of soil microbial communities. No‐tillage compared with plow tillage was functionally enriched for most nutrient cycles. Many other functions integral to soil health can be explored by the PICRUSt omics approach. Soil microbial communities affect the soil's biological, chemical, and physical properties, but there is still a knowledge gap regarding the long‐term impact of tillage practices on soil microbial dynamics. Additionally, the accurate identification of belowground microbial functions is a topic of active interest. In this study, microbial community profiles and functions in soil from a 50‐plus‐year‐old experiment in Ohio, representing one of the world's longest running comparisons of a plow‐tillage system and a continuous no‐tillage system, were compared. The Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) algorithm was used to predict associated functional traits from 16S rRNA gene sequences. Analysis of the sequences revealed a large number of unidentified operational taxonomic units (67%), which is consistent with expectations of the soil ecosystem. Next, we investigated gene and enzyme predictions for nitrogen, sulfur, and methane metabolism and hydrocarbon degradation in soil. Results indicated that no‐tillage was functionally enriched for most nutrient cycles. This study has allowed us to predict distinct functional profiles as a result of legacy land uses. It serves as an example of improved analysis of the functional differences in soil managed by long‐term tillage versus no‐till.

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“…Shiro is one of the most important components of matsutake mushroom formation, and therefore, the microorganisms associated with shiro have been extensively studied. Studies from different continents have suggested that Proteobacteria, Firmicutes and Actinobacteria are favored in T. matsutake shiro soil (Kataoka et al 2012;Kim et al 2014;Oh et al 2016;Vaario et al 2011). Our results were somewhat different from the previous results, as we did not find any significant differences in Actinobacteria between shiro soil and non-shiro soil.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, several studies from different continents have focused on the soil microbial communities in matsutake shiro (Kataoka et al 2012;Kim et al 2014;Lian et al 2006;Oh et al 2016;Usha et al 2011). The development of the shiro is the key for matsutake fruiting body formation.…”

Section: Introductionmentioning

confidence: 99%

“…However, some recent observations suggest the opposite. The molecular screening of 16S rDNA libraries (Boer et al 2005) revealed that a wide repertoire of microbes, including OTUs belonging to the genera Burkholderia, Bacillus, Mycobacterium and Paenibacillus, were significantly more abundant when matsutake was more abundant in soil (Oh et al 2016). The genus Pseudomonas was also found to be abundant in the mycorrhizal roots of T. matsutake sampled from another region (Li et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Bioactive properties of streptomyces may affect the dominance of Tricholoma matsutake in shiro

et al. 2020

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Tricholoma matsutake is known to be the dominant fungal species in matsutake fruitbody neighboring (shiro) soil. To understand the mechanisms behind matsutake dominance, we studied the bacterial communities in matsutake dominant shiro soil and non-shiro soil, isolated the strains of Streptomyces from matsutake mycorrhizal root tips both from shiro soil and from the Pinus densiflora seedlings cultivated in shiro soil. Further, we investigated three Streptomyces spp. for their ability to inhibit fungal growth and Pinus densiflora seedling root elongation as well as two strains for their antifungal and antioxidative properties.Our results showed that Actinobacteria was the most abundant phylum in shiro soil. However, the differences in the Actinobacterial community composition (phylum or order level) between shiro and non-shiro soils were not significant, as indicated by PERMANOVA analyses. A genus belonging to Actinobacteria, Streptomyces, was present on the matsutake mycorrhizas, although in minority. The two antifungal assays revealed that the broths of three Streptomyces spp. had either inhibitory, neutral or promoting effects on the growth of different forest soil fungi as well as on the root elongation of the seedlings. The extracts of two strains, including one isolated from the P. densiflora seedlings, inhibited the growth of either pathogenic or ectomycorrhizal fungi. The effect depended on the medium used to cultivate the strains, but not the solvent used for the extraction. Two Streptomyces spp. showed antioxidant activity in one out of three assays used, in a ferric reducing antioxidant power assay. The observed properties seem to have several functions in matsutake shiro soil and they may contribute to the protection of the shiro area for T. matsutake dominance.

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Distinctive Feature of Microbial Communities and Bacterial Functional Profiles in Tricholoma matsutake Dominant Soil

Cited by 37 publications

References 86 publications

Ectomycorrhization of Tricholoma matsutake with Quercus aquifolioides affects the endophytic microbial community of host plant

Ectomycorrhization of Tricholoma matsutake with Quercus aquifolioides affects the endophytic microbial community of host plant

Functional Predictions of Microbial Communities in Soil as Affected by Long‐term Tillage Practices

Bioactive properties of streptomyces may affect the dominance of Tricholoma matsutake in shiro

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