Distinction of the Skin Flukes &lt;i&gt;Benedenia seriolae&lt;/i&gt; and &lt;i&gt;Neobenedenia girellae&lt;/i&gt; Infecting &lt;i&gt;Seriola&lt;/i&gt; spp. by PCR-RFLP Assay

Kobayshi, Keigo; Khin, Germaine Lau Gek; Shirakashi, Sho; Mukai, Yukinori; Sugihara, Yukitaka; Yanagi, Soetsu; Hirono, Ikuo; Kondo, Hidehiro

doi:10.3147/jsfp.53.124

Cited by 3 publications

(6 citation statements)

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“…This study provides an easy, rapid and useful tool for differential molecular diagnosis of M. sebastis and M. caudata . PCR‐RFLP assays targeting mt DNA or nuclear DNA sequences have been developed for differentiating morphologically similar monogenean species (Kobayashi et al., 2018; Matejusová et al., 2004; Rokicka et al., 2007). In this study, we developed a PCR‐RFLP assay for discriminating two different Microcotyle species and validated the method by examination of a large number of samples collected from S. schlegelii and S. inermis aquaculture farms.…”

Section: Discussionmentioning

confidence: 99%

“…for differentiating morphologically similar monogenean species (Kobayashi et al, 2018;Matejusová et al, 2004;Rokicka et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…28S rDNA and cytochrome b ) have been applied as useful tools for describing new Microcotyle species (Ayadi et al., 2017; Bouguerche et al., 2019a,2019b; Ono et al., 2020; Villora‐Montero et al., 2020). Furthermore, PCR‐RFLP of these genes can provide an accurate, rapid and cost‐effective differential identification tool for morphologically similar monogenean species (Kobayashi et al., 2018; Matejusová et al., 2004; Rokicka et al., 2007 ) . In this study, we developed a PCR‐RFLP strategy for differentiating M. sebastis and M. caudata and validated this method by investigating a large number of monogenean samples collected from cultured S. schlegelii and S. inermis in Korea.…”

Section: Introductionmentioning

confidence: 99%

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Development of a PCR‐RFLP method for differential identification of Microcotyle sebastis and Microcotyle caudata isolated from cultured rockfish in Korea

Nam

Kim

2021

Journal of Fish Diseases

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The genus Microcotyle (Van Beneden & Hesse, 1863) belongs to the polyopisthocotylean monogeneans, which are gill parasites of marine fishes, mainly the perciformes (Ayadi et al., 2017). Infected host fish exhibit anaemia and dyspnoea with considerable mortality but may remain in a state of chronic infections (Kim et al., 2001;Sanz, 1992). The genus Microcotyle is the largest genus in the family Microcotylidae, currently having 69 valid species listed in the WoRMS website; however, it remains a difficult genus for classification (Bouguerche et al., 2019a(Bouguerche et al., ,2019bWoRMS, 2021). Recently, molecular tools have been applied to the Microcotyle taxonomy and several new Microcotyle species have been described by morphological re-examination and molecular analyses (Ayadi et al., 2017;

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Section: Discussionmentioning

confidence: 99%

“…for differentiating morphologically similar monogenean species (Kobayashi et al, 2018;Matejusová et al, 2004;Rokicka et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Development of a PCR‐RFLP method for differential identification of Microcotyle sebastis and Microcotyle caudata isolated from cultured rockfish in Korea

Nam

Kim

2021

Journal of Fish Diseases

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“…In addition to correct species identification (Kobayashi et al, 2018), we need more detailed knowledge on their population structures to establish strategies against parasite infections. In this study, we analyzed the complete mitogenome sequences of 12 Japanese B. seriolae specimens and examined the utility of the mitogenome in population genetics.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Analysis with Complete Mitochondrial Genome Sequences of <i>Benedenia seriolae</i> Specimens Derived from Japanese <i>Seriola</i> spp.

et al. 2019

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Infection with monogenean skin flukes, including Benedenia spp. and Neobenedenia spp., is one of the most important parasitic diseases in Seriola spp. aquaculture worldwide. Skin flukes adhere to host body surface and consume skin tissue. Skin fluke infection itself is not lethal, but affected fish scratch themselves on objects and make skin abrasions, rendering them more susceptible to fatal secondary infections. There is no available vaccination against skin flukes, and the extensive use of anthelmintics might worry consumers. Thus, farmers resort to regular freshwater bathing to remove the parasites from the affected fish and frequently replace and/or clean cage nets to remove parasite eggs. Benedenia seriolae is a major monogenean parasite affecting cultured Seriola spp. (Ogawa et al., 1995; Ogawa, 2015; Ogawa and Shirakashi, 2017). In contrast to Neobenedenia girellae, another major monogenean parasite that affects various marine teleosts, B. seriolae parasitizes exclusively on Seriola spp.

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“…Neobenedenia girellae is also challenging to distinguish from B. seriolae, but egg morphology and minor features of adult parasites may be used on fish farms to aid preliminary identification using microscopy in the absence of molecular tools. 65,66 Morphological differences of these two species are also highlighted in the BeNeZe website and manual (https://beneze.cawthron.org.nz/; Appendix S1).…”

Section: Accounting For Hos T and Par A S Ite Identifi C Ati Onmentioning

confidence: 99%

A decision support tool for parasite management in fish aquaculture

Hutson

Mooney²,

Ernst

et al. 2022

Reviews in Aquaculture

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A decision support tool was developed to aid management of problematic parasites in marine fish aquaculture. The tool provides biologically relevant treatment intervals to interrupt the life cycle of ectoparasitic flatworms that occur in global kingfish and amberjack (Seriola spp.) aquaculture. Temperature‐dependent life cycle parameters for the ‘skin flukes’, Benedenia seriolae and Neobenedenia girellae, and the ‘gill fluke’, Zeuxapta seriolae, were derived from published data and modelled using non‐linear regressions. Increasing temperatures shortened the duration of most life cycle parameters of all parasites. Salinity had no effect on the timing of life cycle parameters but limited hatching success in hypo‐ and hypersaline conditions. The tool, named BeNeZe after the first two letters of each parasite genera, enables rapid determination of treatment intervals for two consecutive medicinal immersion or ‘bathing’ treatments—the first to kill adult flatworms attached to fish and the second to prevent maturity of new parasite recruits. As temperature increases, the interval between treatments and the ‘window’ within which the second treatment should be applied is reduced. The tool can be used for multi‐species infections. The inclusion of parasite taxonomy, biology and behaviour as part of an integrated management strategy is reviewed. Available through an open access app, BeNeZe is intended to be applied in conjunction with farm biosecurity, surveillance, management measures and recognition of independent management units. BeNeZe can be used to reduce infection burdens, improve fish welfare and production and reduce treatment number and frequency.

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Distinction of the Skin Flukes <i>Benedenia seriolae</i> and <i>Neobenedenia girellae</i> Infecting <i>Seriola</i> spp. by PCR-RFLP Assay

Abstract: Capsalid skin flukes are major parasites of amberjacks Seriola spp. worldwide (Ogawa et al., 1995;Ogawa, 2015). They attach to the fish body surface

Cited by 3 publications

References 18 publications

Development of a PCR‐RFLP method for differential identification of Microcotyle sebastis and Microcotyle caudata isolated from cultured rockfish in Korea

Development of a PCR‐RFLP method for differential identification of Microcotyle sebastis and Microcotyle caudata isolated from cultured rockfish in Korea

Phylogenetic Analysis with Complete Mitochondrial Genome Sequences of <i>Benedenia seriolae</i> Specimens Derived from Japanese <i>Seriola</i> spp.

A decision support tool for parasite management in fish aquaculture

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