Distinction between alkaline phosphatase and Mg2+-ATPase of the human polymorphonuclear leukocyte

Long, Gwynn D.; DeChatelet, Lawrence R.; McCall, Charles E.; Bass, David; Cooper, M. Robert

doi:10.1016/0006-2944(79)90041-3

Cited by 4 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The in fluence of freezing on the enzyme activity of blood cells prior to measuring is discussed controversially, not homogenously for all enzymes [13,24,27], and is also dependent on the choice of medium [19], Rate of activity loss at 4°C is about 1% per hour for the investigated enzymes with the exception of G-6-P-DH [6,13]. Therefore G-6-P-DH was measured imme diately.…”

Section: Resultsmentioning

confidence: 99%

“…The liberating effect of detergent on membranebound enzymes is also documented [9, 24, 27, 38, 39, 41], For total disruption of cells, the addition of detergent is unavoidable. From these findings, the scaled differences between our results and those of the above mentioned groups on considerably higher ac tivities of mitochondrial (GLDH), partly mi tochondrial (ASAT, MDH) lysosomal (ACP), and membrane or membrane-associated en [12], or use of plasma gel [27], for obtaining PMNC must be applied. For the lysis of con taminating RBC, NH4CI or hypotonic lysis is used.…”

Section: Enzyme Activitiesmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme Activities in Blood Cells of Man and Dogs after Separation on a Discontinuous Percoli Gradient

Lindena¹,

Sommerfeld²,

Höpfel³

et al. 1983

Enzyme

View full text Add to dashboard Cite

From human and dog peripheral blood, thrombocytes (TRC), mononuclear cells (MNC), polymorphonuclear cells (PMNC), and red blood cells (RBC), were harvested after separation on a discontinuous Percoli gradient in a two-step centrifugation procedure, and 12 enzyme activities were determined in these highly purified cells. The enzyme activities measured are generally severalfold higher than previously reported, a fact which is attributed to the gentle and time-shortening isolation in Percoli, the cell disruption technique using detergent and the enzyme test conditions.

show abstract