Distinct Sensitivity of Normal and Malignant Cells to Ultrasound in Vitro

Lejbkowicz, Flavio; Salzberg, Samuel

doi:10.2307/3433673

Cited by 18 publications

(13 citation statements)

References 29 publications

(32 reference statements)

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“…These sonication parameters are normally used in physiotherapy, in lithotripsy and in aesthetic cavitation, therefore a destructive effect on healthy blood cells can be ruled out. The selectivity (normal cells are more resistant than cancer cells to ultrasonic irradiation) [6], non-invasiveness and low cost make low-intensity ultrasounds attractive to the medical world. Therefore the results seem to lead us toward a non-invasive and effective purging of leukemic cells.…”

Section: Discussionmentioning

confidence: 99%

Study of cellular response induced by frequency change of low intensity ultrasound

Ivone

Pappalettere

Tachibana

2015

2015 E-Health and Bioengineering Conference (EHB)

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U937 human myelomonocytic cells were stressed at variable and fixed frequency by increasing and decreasing mode in a range between 400 kHz and 620 kHz at different PRF (Pulse Repetition Frequency). It was noted that at 10 Hz PRF decreasing mode was more efficient (a higher cell mortality occurred immediately after sonication), while the increasing mode was most efficient at 0.5 Hz. This data were compared with data at fixed frequency in extreme values and center of frequency range (400 kHz, 510 kHz, 620 kHz) at 10 Hz PRF, 180 s duration, 60 V voltage. At this tension the maximum intensity value is 65 mV/cm 2 at 430 kHz. At fixed frequency we found that survival rate was always higher than at sweep frequency. On closer inspection, however we observed that there was a strong dependence by intensity: lowest values of survival rate corresponded to the highest power values. Furthermore a certain dependence of cell mortality from frequency was detected, but it was very detailed compared to that of the power.

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Section: Discussionmentioning

confidence: 99%

Study of cellular response induced by frequency change of low intensity ultrasound

Ivone

Pappalettere

Tachibana

2015

2015 E-Health and Bioengineering Conference (EHB)

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“…On the other hand, the biological effects of relatively low‐intensity ultrasound on malignant tissues and their therapeutic applications are still under investigation. Researchers have shown that low‐intensity ultrasound therapy has great curative potential and can be easily applied to tissues 16 …”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects of Acoustic Cavitation in the Presence of Gold Nanoparticles on a Colon Tumor Model

Sazgarnia

Shanei

Taheri

et al. 2013

Journal of Ultrasound in Medicine

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“…Therefore, this study uses an in vitro vascular model to interrogate the effects of flow rate and sonication intensity on the efficacy of ultrasound treatment. Previous ex vivo models have been used to assess the effects of low‐intensity ultrasound on cells and tissues, but these models lack the ability to control the rate of perfusion . Both the flow rate and the ultrasound parameters can be independently varied within the model, which consists of a three‐dimensional (3D) endothelialized lumen within a collagen type I hydrogel.…”

Section: Introductionmentioning

confidence: 99%

Fluid flow rate dictates the efficacy of low‐intensity anti‐vascular ultrasound therapy in a microfluidic model

2019

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Objective Low‐intensity anti‐vascular ultrasound therapy is an effective means of disrupting the blood supply in the tumor microenvironment. Its diminished effect on the surrounding vasculature is thought to be due to higher blood flow rates outside the tumor that decreases the interaction time between the endothelial lining and the microbubbles, which transduce acoustic energy to thermal heat. However, investigating the effect of circulation rate on the response to low‐intensity ultrasound is complicated by the heterogeneity of the in vivo vascular microenvironment. Here, a 3D microfluidic model is used to directly interrogate the dynamics of ultrasound stimulation. Methods A 3D in vitro vessel consisting of LifeACT transfected endothelial cells facilitate real‐time analysis of actin dynamics during ultrasound treatment. Using an integrated testing platform, both the flow rate of microbubbles within the vessel and the magnitude of insonation can be varied. Results Morphological measurements and dextran transport assays indicate that lower flow rates exacerbate the effect of low‐intensity ultrasound on vessel integrity. Additionally, immunostaining for VE‐cadherin and transmission electron microscopy provide further insight into structural changes in cell‐cell junctions following insonation. Conclusions Overall, these results reveal that blood flow rate is an important parameter to consider during the refinement of anti‐vascular low‐intensity ultrasound therapies.

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Distinct Sensitivity of Normal and Malignant Cells to Ultrasound in Vitro

Abstract: The effect of ultrasonic irradiation on the viability of human normal (foreskin fibroblast and amniotic fluid epithelial) and tumor (breast carcinoma, melanoma, and

Cited by 18 publications

References 29 publications

Study of cellular response induced by frequency change of low intensity ultrasound

Study of cellular response induced by frequency change of low intensity ultrasound

Therapeutic Effects of Acoustic Cavitation in the Presence of Gold Nanoparticles on a Colon Tumor Model

Fluid flow rate dictates the efficacy of low‐intensity anti‐vascular ultrasound therapy in a microfluidic model

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