Distinct Roles of Arrestin 1 Protein in Photoreceptors during Drosophila Development

Shieh, Bih‐Hwa; Kristaponyte, Inga; Yuan, Hong

doi:10.1074/jbc.m114.571224

Cited by 19 publications

(32 citation statements)

References 28 publications

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“…Recently, animal development has been found to be affected by many internal and external factors 31 32 33 . Previous studies have also indicated similar roles of the chemosensory system in other organisms.…”

Section: Discussionmentioning

confidence: 99%

Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta

Cheng

Ling

et al. 2015

Sci Rep

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Chemosensory proteins (CSPs) have been predicted to be involved in development; however, direct evidence for their involvement is lacking, and genetic basis is largely unknown. To determine the function of the chemosensory protein 9 (Si-CSP9) gene in Solenopsis invicta, we used RNA interference to silence Si-CSP9 in 3rd-instar larvae. The 3rd-instar larvae failed to shed their cuticle after being fed Si-CSP9-directed siRNA, and expression profiling of RNAi-treated and untreated control larvae showed that 375 genes were differentially expressed. Pathway enrichment analysis revealed that 4 pathways associated with larval development were significantly enriched. Blast analysis revealed that one fatty acid amide hydrolase (FAAH) gene was up-regulated and 4 fatty acid synthase (FAT) genes and one protein kinase DC2 gene (PKA) were down-regulated in the enriched pathways. Significantly higher expression of these genes was found in 4th-instar larvae, and Pearson correlation analysis of the expression patterns revealed significant relationships among Si-CSP9, PKA, FAAH, and FAT1-4. Moreover, we confirmed that expression levels of Si-CSP9, FAAH, and FAT1-4 were significantly reduced and that the development of 3rd-instar larvae was halted with PKA silencing. These results suggest that Si-CSP9 and PKA may be involved in the network that contributes to development of 3rd-instar larvae.

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Section: Discussionmentioning

confidence: 99%

Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta

Cheng

Ling

et al. 2015

Sci Rep

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“…Both of these isoforms regulate remodeling of the actin cytoskeleton to influence chemotaxis [ 6 – 9 ] and trafficking of specific G-protein coupled receptors [ 10 – 12 ]. The actin cytoskeleton also controls the trafficking and function of ionotropic glutamatergic receptors.…”

Section: Introductionmentioning

confidence: 99%

Beta-arrestin 1 regulation of reward-motivated behaviors and glutamatergic function

et al. 2017

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The two highly homologous non-visual arrestins, beta-arrestin 1 and 2, are ubiquitously expressed in the central nervous system, yet knowledge of their disparate roles is limited. While beta-arrestin 2 (βarr2) has been implicated in several aspects of reward-related learning and behavior, very little is known about the behavioral function of beta-arrestin 1 (βarr1). Using mice lacking βarr1, we focused on the role of this scaffolding and signal transduction protein in reward-motivated behaviors and in striatal glutamatergic function. We found that βarr1 KO mice were both slower in acquiring cocaine self-administration and in extinguishing this behavior. They also showed deficits in learning tasks supported by a natural food reward, suggesting a general alteration in reward processing. We then examined glutamatergic synaptic strength in WT and KO medium spiny neurons (MSNs) of the Nucleus Accumbens (NAc) shell in naïve animals, and from those that underwent cocaine self-administration. An increase in the AMPA/NMDA (A/N) ratio and a relative lack of GluN2B-enriched NMDARs was found in naïve KO vs WT MSNs. Applying Lim Domain Kinase (LIMK1), the kinase that phosphorylates and inactivates cofilin, to these cells, showed that both βarr1 and LIMK regulate the A/N ratio and GluN2B-NMDARs. Cocaine self-administration increased the A/N ratio and GluN2B-NMDARs in WT MSNs and, although the A/N ratio also increased in KO MSNs, this was accompanied by fewer GluN2B-NMDARs and an appearance of calcium-permeable AMPARs. Finally, to examine the consequences of reduced basal GluN2B-NMDARs in reward-processing seen in KO mice, we chronically infused ifenprodil, a GluN2B antagonist, into the NAc shell of WT mice. This intervention substantially reduced food-motivated behavior. Together these findings identify a previously unknown role of βarr1 in regulating specific reward-motivated behaviors and glutamatergic function.

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“…It has been shown that a PDA can be elicited in rdgC null mutant flies with lower intensities of blue light as compared to wild type flies [ 1 , 7 ]. Another possibility to analyze the susceptibility to enter the PDA state is to use recurrent low intensity blue light pulses [ 22 ]. We subjected dark-reared wild type (N = 8), rdgC ΔS (N = 10), rdgC ΔL (N = 8), rdgC ΔSM (N = 8), rdgC 306 (N = 8), and arr2 3 (N = 8) flies to two orange light pulses (~20 μW/cm 2 ) followed by 20 low intensity blue light pulses (~2 μW/cm 2 ) and recorded electroretinograms (ERGs) ( Fig 2A ).…”

Section: Resultsmentioning

confidence: 99%

“…We subjected dark-reared wild type (N = 8), rdgC ΔS (N = 10), rdgC ΔL (N = 8), rdgC ΔSM (N = 8), rdgC 306 (N = 8), and arr2 3 (N = 8) flies to two orange light pulses (~20 μW/cm 2 ) followed by 20 low intensity blue light pulses (~2 μW/cm 2 ) and recorded electroretinograms (ERGs) ( Fig 2A ). arr2 3 flies served as a control because arr2 mutant flies have been already shown to enter the PDA state prematurely under comparable experimental conditions [ 22 ]. To quantify the decrease of recovery with an increasing number of blue light pulses, we measured the amplitudes of the ERG traces in response to each blue light stimulus ( Fig 2B ).…”

Section: Resultsmentioning

confidence: 99%

Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms

et al. 2018

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Drosophila retinal degeneration C (RDGC) is the founding member of the PPEF family of protein phosphatases. RDGC mediates dephosphorylation of the visual pigment rhodopsin and the TRP ion channel. From the rdgC locus, three protein isoforms, termed RDGC-S, -M, and -L, with different N-termini are generated. Due to fatty acylation, RDGC-M and -L are attached to the plasma membrane while RDGC-S is soluble. To assign physiological roles to these RDGC isoforms, we constructed flies that express various combinations of RDGC protein isoforms. Expression of the RDGC-L isoform alone did not fully prevent rhodopsin hyperphosphorylation and resulted in impaired photoreceptor physiology and in decelerated TRP dephosphorylation at Ser936. However, expression of RDGC-L alone as well as RDGC-S/M was sufficient to prevent degeneration of photoreceptor cells which is a hallmark of the rdgC null mutant. Membrane-attached RDGC-M displayed higher activity of TRP dephosphorylation than the soluble isoform RDGC-S. Taken together, in vivo activities of RDGC splice variants are controlled by their N-termini.

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Distinct Roles of Arrestin 1 Protein in Photoreceptors during Drosophila Development

Cited by 19 publications

References 28 publications

Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta

Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta

Beta-arrestin 1 regulation of reward-motivated behaviors and glutamatergic function

Functional characterization of the three Drosophila retinal degeneration C (RDGC) protein phosphatase isoforms

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