Distinct Roles for Dectin-1 and Dectin-2 in Skin Wound Healing and Neutrophilic Inflammatory Responses

Yamagata, Kenji; Emi, Kazuyuki; Tanno, Hiromasa; Sasaki, Ayako; Kitai, Yuki; Miura, Takayuki; Takagi, Naoyuki; Miyake, Shoji; Kasamatsu, Jun; Sato, Ko; Sato, Yuka; Niiyama, Momoko; Goto, Yuhei; Imai, Yoshimichi; Saijo, Shinobu; Iwakura, Yoichiro; Tachi, Masahiro; Kawakami, Kazuyoshi

doi:10.1016/j.jid.2020.04.030

Cited by 16 publications

(12 citation statements)

References 51 publications

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“…The wounded tissues were fixed with 4% paraformaldehyde-phosphate buffer solution and embedded in paraffin as previously described [ 17 , 18 , 19 ]. Sections were harvested from the central portion of the wound and stained with hematoxylin-eosin (HE) according to the standard method.…”

Section: Methodsmentioning

confidence: 99%

Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

et al. 2021

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Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera Lactobacillus and Lactococcus, little has been uncovered on the effects of the genus Enterococcus on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed Enterococcus faecalis KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.

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Section: Methodsmentioning

confidence: 99%

Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

et al. 2021

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“…Dendritic cell-associated C-type lectin (Dectin)-1 and Dectin-2 are typical representatives of the CLR family. 163 , 164 Dectin-1 is a type II transmembrane protein expressed in DCs, macrophages, neutrophils, and monocytes. 165 The extracellular region is a CTLD.…”

Section: Prrs and Ligand-recognition Mechanismsmentioning

confidence: 99%

Pattern recognition receptors in health and diseases

2021

Sig Transduct Target Ther

746

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Pattern recognition receptors (PRRs) are a class of receptors that can directly recognize the specific molecular structures on the surface of pathogens, apoptotic host cells, and damaged senescent cells. PRRs bridge nonspecific immunity and specific immunity. Through the recognition and binding of ligands, PRRs can produce nonspecific anti-infection, antitumor, and other immunoprotective effects. Most PRRs in the innate immune system of vertebrates can be classified into the following five types based on protein domain homology: Toll-like receptors (TLRs), nucleotide oligomerization domain (NOD)-like receptors (NLRs), retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), C-type lectin receptors (CLRs), and absent in melanoma-2 (AIM2)-like receptors (ALRs). PRRs are basically composed of ligand recognition domains, intermediate domains, and effector domains. PRRs recognize and bind their respective ligands and recruit adaptor molecules with the same structure through their effector domains, initiating downstream signaling pathways to exert effects. In recent years, the increased researches on the recognition and binding of PRRs and their ligands have greatly promoted the understanding of different PRRs signaling pathways and provided ideas for the treatment of immune-related diseases and even tumors. This review describes in detail the history, the structural characteristics, ligand recognition mechanism, the signaling pathway, the related disease, new drugs in clinical trials and clinical therapy of different types of PRRs, and discusses the significance of the research on pattern recognition mechanism for the treatment of PRR-related diseases.

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“…Dendritic cell-associated C-type lectin-2 (dectin-2), which is expressed on monocytes, recognizes fungi, activates NF-κB, and induces inflammatory cytokine release [ 92 ]. It has been verified that dectin-2 affects excisional wound healing by regulating the neutrophil inflammatory response and NET formation [ 93 ]. Dectin-2-deficient mice have more collagen deposition, lower levels of MMP-2 and MMP-8, and a shorter healing time than WT mice.…”

Section: Roles Of Nets In Diabetic and Normoglycemic Woundsmentioning

confidence: 99%

The emerging roles of neutrophil extracellular traps in wound healing

Zhu

Ren

et al. 2021

Cell Death Dis

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Delayed wound healing causes problems for many patients both physically and psychologically, contributing to pain, economic burden, loss of function, and even amputation. Although many factors affect the wound healing process, abnormally prolonged or augmented inflammation in the wound site is a common cause of poor wound healing. Excessive neutrophil extracellular trap (NET) formation during this phase may amplify inflammation and hinder wound healing. However, the roles of NETs in wound healing are still unclear. Herein, we briefly introduce NET formation and discuss the possible NET-related mechanisms in wound healing. We conclude with a discussion of current studies, focusing on the roles of NETs in diabetic and normoglycemic wounds and the effectiveness of NET-targeting treatments in wound healing.

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Distinct Roles for Dectin-1 and Dectin-2 in Skin Wound Healing and Neutrophilic Inflammatory Responses

Cited by 16 publications

References 51 publications

Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

Pattern recognition receptors in health and diseases

The emerging roles of neutrophil extracellular traps in wound healing

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