Distinct RhoGEFs Activate Apical and Junctional Contractility under Control of G Proteins during Epithelial Morphogenesis

Bayonas, Alain Garcia De Las; Philippe, Jean‐Marc; Lellouch, Annemarie C.; Lecuit, Thomas

doi:10.1016/j.cub.2019.08.017

Cited by 73 publications

(94 citation statements)

References 82 publications

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“…Our results support this hypothesis, as we observed not only the disappearance of apicomedial actin, but also the beginning of junctional cortical blebbing, which suggests an increase in contractility in the junctional cortical network. These observations highlight the importance of specifically coordinating the junctional cortical and the apicomedial actomyosin networks (Garcia De Las Bayonas et al, 2019).…”

Section: Rho1 Over-expression Induces Rhythmical Cytoskeletal Behaviomentioning

confidence: 88%

Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

Companys

Norris

Bischoff

2020

Journal of Cell Science

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During morphogenesis, cells exhibit various behaviours, such as migration and constriction, which need to be coordinated. How this is achieved remains elusive. During morphogenesis of the Drosophila adult abdominal epidermis, larval epithelial cells (LECs) migrate directedly before constricting apically and undergoing apoptosis. Here, we study the mechanisms underlying the transition from migration to constriction. We show that LECs possess a pulsatile apical actomyosin network, and that a change in network polarity correlates with behavioural change. Exploring the properties of the contractile network, we find that cell contractility, as determined by myosin activity, has an impact on the behaviour of the network, as well as on cytoskeletal architecture and cell behaviour. Pulsed contractions occur only in cells with intermediate levels of contractility. Furthermore, increasing levels of the small Rho GTPase Rho1 disrupts pulsing, leading to cells that cycle between two states, characterised by a junctional cortical and an apicomedial actin network. Our results highlight that behavioural change relies on tightly controlled cellular contractility. Moreover, we show that constriction can occur without pulsing, raising questions why constricting cells pulse in some contexts but not in others.

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Section: Rho1 Over-expression Induces Rhythmical Cytoskeletal Behaviomentioning

confidence: 88%

Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

Companys

Norris

Bischoff

2020

Journal of Cell Science

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“…RhoGEF2 is thought to be particularly important for activating medioapical contractility ( Kerridge et al. , 2016 ; De Las Bayonas et al. , 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Activation of mitotic entry may affect RhoGEF2 localization because medioapical RhoGEF2 is influenced by microtubules and microtubule dynamics change in mitosis ( Rogers et al. , 2004 ; De Las Bayonas et al. , 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Apical Constriction Reversal upon Mitotic Entry Underlies Different Morphogenetic Outcomes of Cell Division

Kalakuntla

Martin

2020

MBoC

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“…Drosophila embryo, RhoGEF2 is the primary RhoA GEF (Barrett et al, 1997;Dawes-334 Hoang et al, 2005;De Las Bayonas et al, 2019;Fox and Peifer, 2007;Häcker and 335 Perrimon, 1998;Kölsch et al, 2007). RhoGEF2 is thought to be particularly important 336 for activating medioapical contractility (De Las Bayonas et al, 2019;Kerridge et al, 337 2016). To promote mitotic rounding, Ect2/Pebble is the primary RhoA activator 338 (Matthews et al, 2012;Rosa et al, 2015).…”

Section: a And B; Video 3) 231mentioning

confidence: 99%

“…RhoGEF2 is influenced by microtubules and microtubule dynamics change in mitosis 343 (De Las Bayonas et al, 2019;Rogers et al, 2004). However, disruption of microtubules 344 does not prevent medioapical myosin activation (Ko et al, 2019).…”

Section: Activation Of Mitotic Entry May Affect Rhogef2 Localization mentioning

confidence: 99%

Apical Constriction Reversal upon Mitotic Entry Underlies Different Morphogenetic Outcomes of Cell Division

Kalakuntla

Martin

2019

Preprint

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1During development, coordinated cell shape changes and cell divisions sculpt 2 tissues. While these individual cell behaviors have been extensively studied, how cell 3 shape changes and cell divisions that occur concurrently in epithelia influence tissue 4 shape is less understood. We addressed this question in two contexts of the early 5Drosophila embryo: premature cell division during mesoderm invagination, and native 6 ectodermal cell divisions with ectopic activation of apical contractility. Using quantitative 7 live-cell imaging, we demonstrated that mitotic entry reverses apical contractility by 8 interfering with medioapical RhoA signaling. While premature mitotic entry inhibits 9 mesoderm invagination, which relies on apical constriction, mitotic entry in an artificially 10 contractile ectoderm induced ectopic tissue invaginations. Ectopic invaginations 11 resulted from medioapical myosin loss in neighboring mitotic cells. This myosin loss 12 enabled non-mitotic cells to apically constrict through mitotic cell stretching. Thus, the 13 spatial pattern of mitotic entry can differentially regulate tissue shape through signal 14 interference between apical contractility and mitosis. 15To determine whether loss of medioapical myosin was a general feature of 131 dividing, contractile epithelial cells, we took advantage of the stereotyped cell divisions 132 in the early mitotic domains that occur on the dorsal side of the head (Foe, 1989), 133 particularly focusing on mitotic domains 1 and 5 ( Figure 2C). We artificially increased 134 ectoderm apical contractility by ectopically expressing folded gastrulation (fog), a ligand 135 for a G-protein-coupled receptor (GPCR) that is expressed in the mesoderm and 136 functions upstream of apical myosin activation (Costa et al., 1994; Dawes-Hoang et al., 137 ,-.

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Distinct RhoGEFs Activate Apical and Junctional Contractility under Control of G Proteins during Epithelial Morphogenesis

Cited by 73 publications

References 82 publications

Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

Coordination of cytoskeletal dynamics and cell behaviour during Drosophila abdominal morphogenesis

Apical Constriction Reversal upon Mitotic Entry Underlies Different Morphogenetic Outcomes of Cell Division

Apical Constriction Reversal upon Mitotic Entry Underlies Different Morphogenetic Outcomes of Cell Division

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