Distinct prognostic value of dynactin subunit 4 (DCTN4) and diagnostic value of DCTN1, DCTN2, and DCTN4 in colon adenocarcinoma

Abstract

BackgroundColon adenocarcinoma (COAD) is ranked as the third most commonly diagnosed cancer in both women and men, and it is the most frequently occurring malignant tumor. Dynactin is a protein compound based on multiple subunits, including dynactin 1–6 (DCTN1–6), in most categories of cytoplasmic dynein performance in eukaryotes. Nevertheless, correlations between the DCTN family and the prognosis and diagnosis of COAD remain unidentified.MethodsStatistics for DCTN mRNA expression in patients with COAD were acquired from The Cancer Genome Atlas. Kaplan–Meier analyses and a Cox regression model were applied to determine overall survival, with computation of HRs and 95% CIs. Several online data portals were used to assess the biological process, and pathway examination was performed using the Kyoto Encyclopedia of Genes and Genomes to predict the biological functionality of DCTN genes.ResultsWe found that high expression of DCTN4 was linked with satisfactory results for overall survival (P=0.042, HR=0.650, 95% CI 0.429–0.985). The expression of DCTN1, DATN2, and DCTN4 was closely correlated with the frequency of colon tumors (P<0.001, area under the curve [AUC]=0.8811, 95% CI 0.8311–0.9312; P<0.001, AUC=0.870, 96% CI 0.833–0.9071; and P=0.0051, AUC=0.6317, 95% CI 0.5725–0.6908, respectively). In the enrichment examination, the level of gene expression was related to the cell cycle, cell apoptosis, and the cell metastasis pathway.ConclusionThe expression levels of DCTN1, DCTN2, and DCTN4 could allow differentiation between cancer-bearing tissues and paracancerous tissue. These genes can be applied as biomarkers to predict the prognosis and diagnosis of COAD.

Identification of a novel glycolysis-related gene signature for predicting metastasis and survival in patients with lung adenocarcinoma

“…With the development of gene signatures, the prognosis of some types of cancer has been predicted by statistical models. DCTN1, DCTN2, and DCTN4 could serve as biomarkers to predict the prognosis and diagnosis of colon adenocarcinoma (COAD) [20]. A novel prognostic biomarker of ovarian cancer was searched and involved a five-DNA methylation marker signature through Cox regression and ROC analyses [21].…”

Study on the Prognostic Values of Dynactin Genes in Low-Grade Glioma

“… 18 Another study showed that high expression of DCTN4 was significantly related to a favorable prognosis in colon adenocarcinoma (COAD) patients. 19 Moreover, it was reported that the mRNA expressions of down-regulated DCTN1 , DCTN2 , DCTN5 , and the up-regulated DCTN6 were associated with a satisfactory prognosis for cutaneous melanoma. 20 However, there are limited reports on the relationship between the prognosis of LGG, as a neurological tumor, and DCTN genes.…”

Down-regulated Solute Carrier Family 4 Member 4 Predicts Poor Progression in Colorectal Cancer

“…As shown in Fig. 6, PPI analysis showed known and predicted interactions interaction between SLC4A4 with SLC9A3, SLC26A6, ENSG00000214921, SLC26A4, DCTN1, AHCYL1, CA4, SLC9A3R1, SLC9A1, and CA2, most of which had been reported be oncogenes or tumor suppressors in cancers (including CRC) [23][24][25][26] .…”

“…To further predict the potential function of SLC4A4 in CRC, using the PPI network, we analyzed the interaction among 82 abnormal expressed genes and found that SLC4A4 might interact with SLC9A3, SLC26A6, ENSG00000214921, SLC26A4, DCTN1, AHCYL1, CA4, SLC9A3R1, SLC9A1, and CA2. Some of these known or predicted interacted genes had been reported to be oncogenes or tumor suppressor genes [23][24][25][26] . For example, SLC9A3 an exchanger of Na + /H + , regulates the transepithelial absorption of Na+ and water and is principally expressed in the apical membranes of the intestinal epithelium, renal proximal tubule, epididymis, and vas deferens [40] .…”