Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus

Zhang, Shengting; Zhao, Yang; Hu, Xiaofeng; Liu, Zongyun; Du, Ji-Zeng

doi:10.1016/j.mce.2013.05.025

Cited by 5 publications

(4 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we compared the difference in the expression levels of IGF-1 and IGFBP-1 in liver and kidney tissues between yaks and lowland cattle. Results showed that both IGF-1 and IGFBP-1 were highly expressed in the liver than in the kidney in the same species ( Figure 3 ), which is in agreement with the findings in previous reports ( Ohlsson et al, 2009 ; Zhang et al, 2013 ). Comparison of the two breeds showed that the expression level of IGF-1 was higher (p<0.05) in the tissues of yaks than that in cattle, indicating that IGF-1 may play an important role in the adaptation to the hypoxic environment.…”

Section: Resultssupporting

confidence: 92%

“…In recent years, the adaptation of plateau mammals to high-altitude hypoxia has attracted increasing attention. Numerous species on the Tibet plateau have been examined to explore the hypoxia-related molecular mechanisms ( Wei et al, 2006 ; Cao et al, 2008 ; Cao et al, 2009 ; Zhang et al, 2013 ), but little information about yaks is available. Yak ( Bos grunniens ) is the most important animal species in the Qinghai-Tibet plateau not only because of its economic value in meat, milk, and usage in agriculture, but also as an ideal model animal for studying the molecular mechanisms of adapting to the cold and low-oxygen environment ( Hu et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization and Expression Analysis of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-1 Genes in Qinghai-Tibet Plateau Bos grunniens and Lowland Bos taurus

Chen

Lan

et al. 2014

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

Insulin-like growth factor-1 (IGF-1) and insulin-like growth factor binding protein-1 (IGFBP-1) play a pivotal role in regulating cellular hypoxic response. In this study, we cloned and characterized the genes encoding IGF-1 and IGFBP-1 to improve the current knowledge on their roles in highland Bos grunniens (Yak). We also compared their expression levels in the liver and kidney tissues between yaks and lowland cattle. We obtained full-length 465 bp IGF-1 and 792 bp IGFBP-1, encoding 154 amino acids (AA) IGF-1, and 263 AA IGFBP-1 protein, respectively using reverse transcriptase-polyerase chain reaction (RT-PCR) technology. Analysis of their corresponding amino acid sequences showed a high identity between B. grunniens and lowland mammals. Moreover, the two genes were proved to be widely distributed in the examined tissues through expression pattern analysis. Real-time PCR results revealed that IGF-1 expression was higher in the liver and kidney tissues in B. grunniens than in Bos taurus (p<0.05). The IGFBP-1 gene was expressed at a higher level in the liver (p<0.05) of B. taurus than B. grunniens, but it has a similar expression level in the kidneys of the two species. These results indicated that upregulated IGF-1 and downregulated IGFBP-1 are associated with hypoxia adaptive response in B. grunniens.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Molecular Characterization and Expression Analysis of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-1 Genes in Qinghai-Tibet Plateau Bos grunniens and Lowland Bos taurus

Chen

Lan

et al. 2014

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

show abstract

“…For instance, IGFBP1 potently promoted β-cell regeneration by potentiating α-to β-cell transdifferentiation in zebrafish [53]. Moreover, hypoxia significantly up-regulated hepatic Igfbp1 expression in the liver [54]. In the present study, we observed a higher expression of IGFBP1 in the fast-growth fish than in the slow-growth fish.…”

Section: Plos Onesupporting

confidence: 60%

Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion

2020

View full text Add to dashboard Cite

During recent years, China has become a hotspot for the domestication of mandarin fish, and this is of great commercial value. Although the food preference of domesticated mandarin fish has been studied, little is known about genes regulating their growth. We raised hybrid mandarin fish on artificial feed for 3 months, the results showed that the survival rate of hybrid mandarin fish was 60.00%. Their total length and body weight were 18.34 ±0.43 cm and 100.44 ±4.87 g. The absolute length and weight gain rates were 0.14 cm/d and 1.08 g/d, respectively. Finally, RNA sequencing (RNA-Seq) was performed to identify potential genes and pathways activated in response to growth performance. The transcriptome analysis generated 68, 197 transcripts and 45,871 unigenes. Among them, 1025 genes were up-regulated and 593 genes were down-regulated between the fast-and slow-growth fish. Finally, we obtained 32 differentially expressed genes, which were mainly related to fatty acid biosynthesis (e.g. FASN and ACACB), collecting duct acid secretion (e.g. ATP6E and KCC4), cell cycle (e.g. CDC20 and CCNB), and the insulin-like growth factor (IGF) system (IGFBP1). These pathways might be related to the growth of hybrid mandarin fish. In addition, more potential single nucleotide polymorphisms (SNPs) were detected in the fastgrowth fish than in the slow-growth fish. The results suggest that the interaction of metabolism and abundant alleles might determine the growth of hybrid mandarin fish after food conversion.

show abstract

“…It is possible that the morphology, blood properties, physiology, biochemistry and gene product structure and function of small mammals living on plateaus or in underground caves may have changed as they adapted to life in a low oxygen environment. [46][47][48][49][50][51][52][53][54] For example, some subterranean mammals that live for long periods of time in anoxic and dark caves have specialized sensory systems, such as specialized circadian rhythms, [55][56][57] heightened hearing, [58][59][60] degraded vision, 55,59,[61][62][63] and oxyosphresia 64 (Table 1).…”

Section: Physiochemical Properties Related To Adaptation To Hypoxic Environmentsmentioning

confidence: 99%

The hypoxia adaptation of small mammals to plateau and underground burrow conditions

Pan

Sun

et al. 2021

Anim Models and Exp Med

View full text Add to dashboard Cite

Oxygen is a key factor in the growth, reproduction and metabolism of aerobic organisms 1 and oxygen content varies due to environmental factors, such as temperature, humidity, atmospheric pressure and altitude. 2 O 2 deficiencies may occur in environments of land-living animals such as high altitudes 3 and underground caves. 4 Although mammals are largely intolerant of hypoxia, there are a few rodent species that live in hypoxic niches. These animals have evolved complex physiological and molecular adaptive systems that enable them to survive in hypoxic environments. 5 Low O 2 can lead to an increase in the production of reactive oxygen species (ROS) in the organism, 6 which in turn triggers oxidative stress. 7,8 However, antioxidant defense systems in organisms can counteract the adverse effects of ROS. 9 Maintaining a balance between energy production and consumption is also key to tolerating hypoxia. 10,11 In general, in order to adapt to hypoxia, animals can produce energy through anaerobic metabolism to maintain their metabolism, 12 and when the O 2 supply is limited, the metabolic rate of most hypoxiatolerant animals shows a strong decline. 5,13 In hypoxia-tolerant newborn mammals, oxygen consumption (VO 2 ) was shown not to exceed the baseline level during reoxygenation after hypoxia (15% O 2 ), and rapidly returned to the pre-hypoxia level, and lactate accumulation was observed only in more severe hypoxia (10% O 2 ). 14

show abstract

Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus

Cited by 5 publications

References 59 publications

Molecular Characterization and Expression Analysis of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-1 Genes in Qinghai-Tibet Plateau Bos grunniens and Lowland Bos taurus

Molecular Characterization and Expression Analysis of Insulin-like Growth Factor-1 and Insulin-like Growth Factor Binding Protein-1 Genes in Qinghai-Tibet Plateau Bos grunniens and Lowland Bos taurus

Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion

The hypoxia adaptation of small mammals to plateau and underground burrow conditions

Contact Info

Product

Resources

About