Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Galvez, Eric; Iljazović, Aida; Amend, Lena; Lesker, Till Robin; Renault, Thibaud T.; Thiemann, Sophie; Hao, Lianxu; Roy, Urmi; Gronow, Achim; Charpentier, Emmanuelle; Strowig, Till

doi:10.1016/j.chom.2020.09.012

Cited by 106 publications

(64 citation statements)

References 83 publications

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“…Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates [37]. As revealed by several studies, Prevotella spp.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides of Sporoderm-Broken Spore of Ganoderma lucidum Modulate Adaptive Immune Function via Gut Microbiota Regulation

Zhang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Ganoderma lucidum (Leyss.Fr.) Karst is one of the well-known medicinal macrofungi all over the world, and mounting researches have focused on the polysaccharides derived from the spores of G. lucidum. In the present study, BALB/c mice (n = 8–10) were administered with crude polysaccharides of G. lucidum spores (CPGS) and the refined polysaccharides of G. lucidum spores (RPGS) for 30 days to investigate their effect on the adaptive immune system. Results showed that CPGS and RPGS displayed diverse effects on the lymphocyte activity in the spleen. The splenocyte proliferation activity upon mitogen was suppressed by CPGS and RPGS, while the NK cell’s tumor-killing ability was promoted by CPGS. Both CPGS and RPGS could increase the proportion of naïve T cells in thymus, but only RPGS significantly uplifted the percentage of T cells, as well as the T cell subsets, in peripheral blood, and promoted the activation by upregulating the expression of costimulatory factor CD28. Moreover, 16S sequencing results showed that the effects of CPGS and RPGS were closely related to the regulation of gut microbiota. β-diversity of the microbiome was evidently changed by CPGS and RPGS. The phytoestrogen/polysaccharide-metabolizing bacteria (Adlercreutzia, Parabacteroides, and Prevotella), and an unclassified Desulfovibrionaceae, were remarkably enriched by CPGS or RPGS, and functions involving carbohydrate metabolism, membrane transport, and lipid metabolism were regulated. Moreover, the enrichments of Adlercreutzia, Prevotella, and Desulfovibrionaceae were positively related to the immune regulation by CPGS and RPGS, while that of Parabacteroides displayed a negative correlation. These findings suggested a promising effect of the polysaccharide from sporoderm-broken spore of G. lucidum in immune regulation to promote health control.

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“…Prevotella spp. exhibit variability in the utilization of diverse complex carbohydrates [37]. As revealed by several studies, Prevotella spp.…”

Section: Discussionmentioning

confidence: 99%

Polysaccharides of Sporoderm-Broken Spore of Ganoderma lucidum Modulate Adaptive Immune Function via Gut Microbiota Regulation

Zhang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…However, H. pylori is unable to activate NKG2D-NKG2DL system and IL−15 expression (Montalban-Arques et al, 2016). Prevotella is the predominant bacterial genus in the human gut microbiome (Gálvez et al, 2020). P. copri has a proinflammatory function in several diseases that involves enhancing resistance to hostderived ROS and producing the redox protein thioredoxin (Hofer, 2014).…”

Section: Non-h Pylori Bacteria That Promote Gastric Carcinogenesismentioning

confidence: 99%

Role of the Gastric Microbiome in Gastric Cancer: From Carcinogenesis to Treatment

et al. 2021

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The development of sequencing technology has expanded our knowledge of the human gastric microbiome, which is now known to play a critical role in the maintenance of homeostasis, while alterations in microbial community composition can promote the development of gastric diseases. Recently, carcinogenic effects of gastric microbiome have received increased attention. Gastric cancer (GC) is one of the most common malignancies worldwide with a high mortality rate. Helicobacter pylori is a well-recognized risk factor for GC. More than half of the global population is infected with H. pylori, which can modulate the acidity of the stomach to alter the gastric microbiome profile, leading to H. pylori-associated diseases. Moreover, there is increasing evidence that bacteria other than H. pylori and their metabolites also contribute to gastric carcinogenesis. Therefore, clarifying the contribution of the gastric microbiome to the development and progression of GC can lead to improvements in prevention, diagnosis, and treatment. In this review, we discuss the current state of knowledge regarding changes in the microbial composition of the stomach caused by H. pylori infection, the carcinogenic effects of H. pylori and non-H. pylori bacteria in GC, as well as the potential therapeutic role of gastric microbiome in H. pylori infection and GC.

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“…The prevalence and relative abundance of P. copri has been linked to plant-rich diets in humans and mouse models (De Filippo et al, 2010; Fragiadakis et al, 2019; Gálvez et al, 2020; Kovatcheva-Datchary et al, 2019; Ruengsomwong et al, 2016; Wu et al, 2011), yet the underlying molecular mechanism is still poorly understood. To demonstrate the utility of our gene insertion system in identifying gene functions, we decided to investigate the genetic basis for utilization of distinct carbohydrates in P. copri .…”

Section: Resultsmentioning

confidence: 99%

“…The prediction of PULs in P. copri genomes and MAGs was described previously (Gálvez et al, 2020). Briefly, PULs and susC/D -like gene annotations were carried out using PULpy (Stewart et al, 2018) (commit 8955cdb, https://github.com/WatsonLab/PULpy).…”

Section: Star Methodsmentioning

confidence: 99%

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A versatile genetic toolbox forPrevotella coprienables studying polysaccharide utilization systems

Galvez

Amend

et al. 2021

Preprint

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Prevotella copri is a prevalent inhabitant of the human gut and has been associated with plant-rich diet consumption and diverse health states. The underlying genetic basis of these associations remains enigmatic due to the lack of genetic tools. Here, we developed a novel versatile genetic toolbox for rapid and efficient genetic insertion and allelic exchange applicable to P. copri strains from multiple clades. Enabled by the genetic platform, we systematically investigated the specificity of polysaccharide utilization loci (PULs), and identified four highly conserved PULs for utilizing arabinan, pectic galactan, arabinoxylan and inulin, respectively. Further genetic and functional analysis of arabinan utilization systems illustrate that P. copri has evolved two distinct types of arabinan-processing PULs (PULAra) and that the type-II PULAra is significantly enriched in individuals consuming a vegan diet compared to other diets. In summary, this genetic toolbox will enable functional genetic studies for P. copri in the future.

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Distinct Polysaccharide Utilization Determines Interspecies Competition between Intestinal Prevotella spp.

Cited by 106 publications

References 83 publications

Polysaccharides of Sporoderm-Broken Spore of Ganoderma lucidum Modulate Adaptive Immune Function via Gut Microbiota Regulation

Polysaccharides of Sporoderm-Broken Spore of Ganoderma lucidum Modulate Adaptive Immune Function via Gut Microbiota Regulation

Role of the Gastric Microbiome in Gastric Cancer: From Carcinogenesis to Treatment

A versatile genetic toolbox forPrevotella coprienables studying polysaccharide utilization systems

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