“…We performed this protocol and analysis on six fresh-frozen tissue sections of resected human brain metastases ( Table 1 ). These samples were drawn from four primary tumor types with varying levels of T cell infiltration ( Sudmeier et al., 2022 ).…”
Section: Expected Outcomesmentioning
confidence: 99%
“… Figure 3 TCRβ sequences obtained from Visium are recapitulated by single-cell RNA-sequencing (A) Correlation of TCR sequences obtained from the assay with expression of CD3E (a pan-T cell marker) by Visium gene expression analysis. (B) For four patients, single cell RNA-sequencing (scRNA-seq) on T cells was performed to validate TCR sequences obtained by the spatial assay ( Sudmeier et al., 2022 ). Pie charts show frequency of clones obtained from the spatial assay and are colored based on whether the clones were independently found through scRNA-seq in CD4 + or CD8 + T cells.…”
Section: Expected Outcomesmentioning
confidence: 99%
“… (B) For four patients, single cell RNA-sequencing (scRNA-seq) on T cells was performed to validate TCR sequences obtained by the spatial assay ( Sudmeier et al., 2022 ). Pie charts show frequency of clones obtained from the spatial assay and are colored based on whether the clones were independently found through scRNA-seq in CD4 + or CD8 + T cells.…”
Section: Expected Outcomesmentioning
confidence: 99%
“…This technique enables detailed studies of the spatial organization of the human T cell repertoire, such as the localization of infiltrating T cell clones within the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Sudmeier et al. (2022) .…”
mentioning
confidence: 99%
“…For complete details on the use and execution of this protocol, please refer to Sudmeier et al. (2022) .…”
“…We performed this protocol and analysis on six fresh-frozen tissue sections of resected human brain metastases ( Table 1 ). These samples were drawn from four primary tumor types with varying levels of T cell infiltration ( Sudmeier et al., 2022 ).…”
Section: Expected Outcomesmentioning
confidence: 99%
“… Figure 3 TCRβ sequences obtained from Visium are recapitulated by single-cell RNA-sequencing (A) Correlation of TCR sequences obtained from the assay with expression of CD3E (a pan-T cell marker) by Visium gene expression analysis. (B) For four patients, single cell RNA-sequencing (scRNA-seq) on T cells was performed to validate TCR sequences obtained by the spatial assay ( Sudmeier et al., 2022 ). Pie charts show frequency of clones obtained from the spatial assay and are colored based on whether the clones were independently found through scRNA-seq in CD4 + or CD8 + T cells.…”
Section: Expected Outcomesmentioning
confidence: 99%
“… (B) For four patients, single cell RNA-sequencing (scRNA-seq) on T cells was performed to validate TCR sequences obtained by the spatial assay ( Sudmeier et al., 2022 ). Pie charts show frequency of clones obtained from the spatial assay and are colored based on whether the clones were independently found through scRNA-seq in CD4 + or CD8 + T cells.…”
Section: Expected Outcomesmentioning
confidence: 99%
“…This technique enables detailed studies of the spatial organization of the human T cell repertoire, such as the localization of infiltrating T cell clones within the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Sudmeier et al. (2022) .…”
mentioning
confidence: 99%
“…For complete details on the use and execution of this protocol, please refer to Sudmeier et al. (2022) .…”
Single cell RNA sequencing (scRNA‐seq) provides a great convenience for studying tumor occurrence and development for its ability to study gene expression at the individual cell level. However, patient‐derived tumor tissues are composed of multiple types of cells including tumor cells and adjacent non‐malignant cells such as stromal cells and immune cells. The spatial locations of various cells in situ tissues plays a pivotal role in the occurrence and development of tumors, which cannot be elucidated by scRNA‐seq alone. Spatially resolved transcriptomics (SRT) technology emerges timely to explore the unrecognized relationship between the spatial background of a particular cell and its functions, and is increasingly used in cancer research. This review provides a systematic overview of the SRT technologies that are developed, in particular the more widely used cutting‐edge SRT technologies based on next‐generation sequencing (NGS). In addition, the main achievements by SRT technologies in precisely unveiling the underappreciated spatial locations on gene expression and cell function with unprecedented high‐resolution in cancer research are emphasized, with the aim of developing more effective clinical therapeutics oriented to a deeper understanding of the interaction between tumor cells and surrounding non‐malignant cells.
For tumor treatment, the ultimate goal in tumor therapy is to eliminate the primary tumor, manage potential metastases, and trigger an antitumor immune response, resulting in the complete clearance of all malignant cells. Tumor microenvironment (TME) refers to the local biological environment of solid tumors and has increasingly become an attractive target for cancer therapy. Neutrophils within TME of gastric cancer (GC) spontaneously undergo ferroptosis, and this process releases oxidized lipids that limit T cell activity. Enhanced photodynamic therapy (PDT) mediated by di‐iodinated IR780 (Icy7) significantly increases the production of reactive oxygen species (ROS). Meanwhile, neutrophil ferroptosis can be triggered by increased ROS generation in the TME. In this study, a liposome encapsulating both ferroptosis inhibitor Liproxstatin‐1 and modified photosensitizer Icy7, denoted LLI, significantly inhibits tumor growth of GC. LLI internalizes into MFC cells to generate ROS causing immunogenic cell death (ICD). Simultaneously, liposome‐deliver Liproxstatin‐1 effectively inhibits the ferroptosis of tumor neutrophils. LLI‐based immunogenic PDT and neutrophil‐targeting immunotherapy synergistically boost the anti‐PD‐1 treatment to elicit potent TME and systemic antitumor immune response with abscopal effects. In conclusion, LLI holds great potential for GC immunotherapy.
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