Distinct Patterns of Rhizosphere Microbiota Associated With Rice Genotypes Differing in Aluminum Tolerance in an Acid Sulfate Soil

Abstract

Rhizosphere microbes are important for plant tolerance to various soil stresses. Rice is the most aluminum (Al)-tolerant small grain cereal crop species, but the link between rice Al tolerance and rhizosphere microbiota remains unclear. This study aimed to investigate the microbial community structure of aluminum-sensitive and Al-tolerant rice varieties in acid sulfate soil under liming and non-liming conditions. We analyzed the rice biomass and mineral element contents of rice plants as well as the chemical properties and microbial (archaea, bacteria, and fungi) communities of rhizosphere and bulk soil samples. The results showed that the Al-tolerant rice genotype grew better and was able to take up more phosphorus from the acid sulfate soil than the Al-sensitive genotype. Liming was the main factor altering the microbial diversity and community structure, followed by rhizosphere effects. In the absence of liming effects, the rice genotypes shifted the community structure of bacteria and fungi, which accounted for the observed variation in the rice biomass. The Al-tolerant rice genotype recruited specific bacterial and fungal taxa (Bacillus, Pseudomonas, Aspergillus, and Rhizopus) associated with phosphorus solubilization and plant growth promotion. The soil microbial co-occurrence network of the Al-tolerant rice genotype was more complex than that of the Al-sensitive rice genotype. In conclusion, the bacterial and fungal community in the rhizosphere has genotype-dependent effects on rice Al tolerance. Aluminum-tolerant rice genotypes recruit specific microbial taxa, especially phosphorus-solubilizing microorganisms, and are associated with complex microbial co-occurrence networks, which may enhance rice growth in acid sulfate soil.

Different Functional and Taxonomic Composition of the Microbiome in the Rhizosphere of Two Purslane Genotypes

“…These differences were easily visualized in the NMDS ordination plot (Figures 1 and 2) where it has been shown the important role of a plant genotype as key determinant of the microbial assemblage. Similar results were shown in different specie varieties of maize [24], barley [25], wheat [26], soybean [27], cotton [28], or rice [29]. Nonetheless, there was no significant effect of the purslane genotype on alpha diversity (Shannon index and ASV richness), either for bacteria or fungi, except for fungal richness that was significantly higher in the Spanish (wild) purslane rhizosphere (F = 18.4, p < 0.001).…”

Continuous-cropping-tolerant soybean cultivars alleviate continuous cropping obstacles by improving structure and function of rhizosphere microorganisms

“…Frontiers in Microbiology frontiersin.org soil microbial diversity and community structure (Huang et al, 2022;Xiao et al, 2022). In this study, it was found that root microorganisms were significantly different among different soybean cultivars.…”

“…The different genotypes had significantly different effects on soil microorganisms, and the different genotypes would also affect soil microbial diversity and community structure ( Huang et al, 2022 ; Xiao et al, 2022 ). In this study, it was found that root microorganisms were significantly different among different soybean cultivars.…”

Stochastic processes drive the soil fungal communities in a developing mid-channel bar

“…Though many studies have reported the species-specific selection of rhizosphere microbial communities by plants through root traits and exudates ( Costa et al, 2006 ; Sweeney et al, 2021 ; Xiao et al, 2022 ), soil physicochemical properties were considered to be the primary factors affecting rhizosphere fungal community than plant species type (identity) in some studies ( Singh et al, 2007 ; Chen et al, 2019 ). Soil can regulate the rhizosphere microbial community; particularly, soil pH and nutrients are regarded as the key factors influencing the structure of the rhizosphere microbial community ( Philippot et al, 2013 ).…”