Distinct patterns of autophagy evoked by two benzoxazine derivatives in vascular endothelial cells

Wang, Li; Dong, Zhiwu; Huang, Bin; Zhao, Bao‐Xiang; Wang, Hua; Zhao, Jing; Kung, Hsiang‐Fu; Zhang, ShangLi; Jiang, Miao

doi:10.4161/auto.6.8.13508

Cited by 51 publications

(41 citation statements)

References 35 publications

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“…mTOR activation enhances protein translation by phosphorylating its targets p70S6K and 4EBP1 and inhibiting the induction of autophagy [23] . Our results showed that 3,4,4'-THS suppressed the phosphorylation of p70S6K and 4EBP1, indicating that mTOR signaling may be involved in 3,4,4'-THS-induced autophagy.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol analogue 3,4,4′-trihydroxy-trans-stilbene induces apoptosis and autophagy in human non-small-cell lung cancer cells in vitro

et al. 2015

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Aim:To investigate the effects of 3,4,4′-trihydroxy-trans-stilbene (3,4,4′-THS), an analogue of resveratrol, on human non-small-cell lung cancer (NSCLC) cells in vitro. Methods: Cell viability of NSCLC A549 cells was determined by MTT assay. Cell apoptosis was evaluated using flow cytometry and TUNEL assay. Cell necrosis was evaluated with LDH assay. The expression of apoptosis-or autophagy-associated proteins was measured using Western blotting. The formation of acidic compartments was detected using AO staining, neutral red staining and Lysotracker-Red staining. LC3 punctae were analyzed with fluorescence microscopy. Results: Treatment with 3,4,4′-THS (10-80 μmol/L) concentration-dependently inhibited the cell viability. It did not cause cell necrosis, but induced apoptosis accompanied by up-regulation of cleavaged PARP, caspase3/9 and Bax, and by down-regulation of Bcl-2 and surviving. It also increased the formation of acidic compartments, LC3-II accumulation and GFP-LC3 labeled autophagosomes in the cells. It inhibited the mTOR-dependent pathway, but did not impair autophagic flux. 3,4,4′-THS-induced cell death was enhanced by the autophagy inhibitors 3-MA (5 mmol/L) or Wortmannin (2 μmol/L). Moreover, 3,4,4′-THS treatment elevated the ROS levels in the cells, and co-treatment with 3-MA further elevated the ROS levels. 3,4,4′-THS-induced apoptosis and autophagy in the cells was attenuated by NAC (10 mmol/L) Conclusion: 3,4,4′-THS induces both apoptosis and autophagy in NSCLC A549 cells in vitro. Autophagy inhibitors promote 3,4,4′-THSinduced apoptosis of A549 cells, thus combination of 3,4,4′-THS and autophagy inhibitor provides a promising strategy for NSCLC treatment.

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Section: Discussionmentioning

confidence: 99%

Resveratrol analogue 3,4,4′-trihydroxy-trans-stilbene induces apoptosis and autophagy in human non-small-cell lung cancer cells in vitro

et al. 2015

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“…RT-qPCR and western blot analysis demonstrated that the mRNA and protein expression of LC3II and ATG5 were significantly increased, whereas LC3I and SQSTM1 mRNA and protein expression levels were significantly decreased in pWHL-1-transfected HBECs. An increased ratio of LC3II/LC3I is a marker of an enhancement in autophagosomes formation, whereas SQSTM1 expression is negatively correlated with autophagy (26). ATG5 is involved in the early stage of autophagosome formation and has multiple functions in various physiological contexts (27).…”

Section: Discussionmentioning

confidence: 99%

Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells

Deng

Liu

Yang

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Human bocavirus (HBoV) is classified in the Bocavirus genus within the Parvoviridae family, first identified from children with respiratory diseases. Previous studies have investigated the stimulating effect of HBoV on cell apoptosis and autophagy. In the present study, human bronchial epithelial cells (HBECs) were utilized to examine the mechanism of HBoV recombination expressing vector (pWHL-1) on the promotion of cell apoptosis and autophagy. The results from the present study indicated that pWHL-1 inhibited the proliferation of HBECs in a time-dependent manner. Additionally, pWHL-1induced apoptosis, as substantiated by an increased apoptotic rate and presence of autophagosomes. Following pWHL-1 transfection, proliferating cell nuclear antigen, caspase-3 and B cell lymphoma 2 (Bcl-2) protein expression levels were decreased, with the exception of Bcl-2 associated x (Bax) protein, which increased. mRNA and protein expression levels of microtubule-associated protein 1A/1B-light chain 3 (LC3) II and autophagy protein 5 were increased in pWHL-1-transfected HBECs, whereas, the mRNA and protein levels of LC3I and sequestosome 1 were decreased. Notably, pWHL-1 also enhanced the activation of p53 and inhibited AKT activation in HBECs. Results from the present study suggest that pWHL-1 induces apoptosis and autophagy, thus providing a novel insight into the effect of HBoV and its uses in respiratory diseases.

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“…ANXA7 siRNA was designed and custom synthesized by Invitrogen as previously reported (Wang et al, 2010). When cell density reached 50-60% confluence, 80 nM siRNA against TIA1 or ANXA7 and scramble siRNA (negative control) were transfected into cells with RNAiFect Transfection Reagent according to the manufacturer's protocol (QIAGEN, 301605).…”

Section: Rna Interferencementioning

confidence: 99%

“…2C and D). Furthermore, ANXA7 interference strategy was performed as described previously (Wang et al, 2010). As shown in Fig.…”

Section: Suppressing Anxa7 Gtpase Activity By Abo Inhibited Phosphorymentioning

confidence: 99%

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TIA1 interacts with annexin A7 in regulating vascular endothelial cell autophagy

Huang

Liu

et al. 2014

The International Journal of Biochemistry & Cell Biology

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Distinct patterns of autophagy evoked by two benzoxazine derivatives in vascular endothelial cells

Cited by 51 publications

References 35 publications

Resveratrol analogue 3,4,4′-trihydroxy-trans-stilbene induces apoptosis and autophagy in human non-small-cell lung cancer cells in vitro

Resveratrol analogue 3,4,4′-trihydroxy-trans-stilbene induces apoptosis and autophagy in human non-small-cell lung cancer cells in vitro

Human bocavirus induces apoptosis and autophagy in human bronchial epithelial cells

TIA1 interacts with annexin A7 in regulating vascular endothelial cell autophagy

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