Distinct pathways of adaptive evolution in Cryptococcus neoformans reveal a mutation in adenylyl cyclase with trade-offs for pathogenicity

Hilbert, Zoë A.; Bednarek, Joseph M.; Schwiesow, Mara J.W.; Chung, Krystal Y.; Moreau, Christian T.; Brown, Jessica C.S.; Elde, Nels C.

doi:10.1016/j.cub.2023.08.054

Cited by 1 publication

(3 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Colonies were genotyped by colony PCR following previously published protocols 34 . Genomic sequences were amplified from gDNA extracted from candidate colonies and integration of the drug cassette at the SH2 locus was confirmed by Sanger sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…PCRs were also performed to ensure that no strains had integrated the gRNA or CAS9 constructs. All primer sequences for generating CRISPR reagents and genotyping mutants have been previously published 34 .…”

Section: Methodsmentioning

confidence: 99%

“…A chromosome scale assembly of the parental Ftc239-1 strain was drafted largely as previously published 34 . Briefly, fast5 files from Nanopore sequencing were re-basecalled using guppy-gpu (v6.4.2) and the resulting fastq files were assembled using Canu (v 2.1.1) with an estimated genome size of 20 megabases and default settings 38 .…”

Section: Sequencing Analysismentioning

confidence: 99%

See 2 more Smart Citations

Distinct routes to thermotolerance in the fungal pathogenCryptococcus neoformans

Schwiesow,

Elde,

Hilbert

2024

Preprint

Self Cite

View full text Add to dashboard Cite

Increasing temperatures associated with climate change have the potential for far-reaching impact on human health and disease vectors, including fungal pathogens. Pathogenic fungi occupy a wide range of environments across the world, and their ranges have been slowly expanding in recent decades due, in part, to climate change. Despite these links between increasing temperature and higher prevalence of fungal disease, the direct effects of rising environmental temperatures on the evolution of pathogenic fungi remains unclear. In this study, we investigated how increasing temperatures drive adaptive evolution in the human fungal pathogen Cryptococcus neoformans. First, we performed serial passages of a C. neoformans environmental isolate with gradual changes in temperature over the course of ~40 days. Through this approach we identified several distinct thermally adapted isolates with competitive growth advantages over the parental strain at high temperatures. We then characterized the phenotypic and genetic changes acquired in several evolved isolates, which include alteration of cell size, colony morphology, and, surprisingly, antifungal resistance. These results highlight the remarkable flexibility of fungi to adapt rapidly to new environments and raise pressing questions about the impacts of rising environmental temperatures on the future of infectious diseases and human health.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Sequencing Analysismentioning

confidence: 99%

See 1 more Smart Citation

Distinct routes to thermotolerance in the fungal pathogenCryptococcus neoformans

Schwiesow,

Elde,

Hilbert

2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Distinct pathways of adaptive evolution in Cryptococcus neoformans reveal a mutation in adenylyl cyclase with trade-offs for pathogenicity

Cited by 1 publication

References 87 publications

Distinct routes to thermotolerance in the fungal pathogenCryptococcus neoformans

Distinct routes to thermotolerance in the fungal pathogenCryptococcus neoformans

Contact Info

Product

Resources

About