Distinct modes of molecular regulation of CCL3 induced calcium flux in monocytic cells

Abstract

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Constitutive lysosome exocytosis releases ATP and engages P2Y receptors in human monocytes

“…1F). These findings are consistent with the identification of functional ryanodine receptors in monocytes by others (Bracci et al, 2007;Cardaba and Mueller, 2009;Guidarelli et al, 2009 Scavenging extracellular ATP/ADP using apyrase severely attenuates Ca 2+ responses evoked by GPN (Fig. 3).…”

Rac1 plays a role in CXCL12 but not CCL3-induced chemotaxis and Rac1 GEF inhibitor NSC23766 has off target effects on CXCR4

“…MCF-7 cells were harvested and treated as described previously [29]. Cells were loaded with Fura-2 as described previously [30,31]. Inhibitors were present during the 30 mins incubation period.…”

Simvastatin-induced up-regulation of gap junctions composed of connexin 43 sensitize Leydig tumor cells to etoposide: An involvement of PKC pathway

“…It is well known that intracellular calcium plays a key role in PKC activation. Studies showed that release of intracellular calcium was prevented by simvastatin treatment in some cells (Cardaba and Mueller, 2009), which raised the possibility to target calcium as a regulatory signal. Alternatively, inhibition of HMG-CoA reductase may reduce mevalonate and its downstream metabolites which have shown significant influence in many intracellular processes.…”