Distinct Mobilization of Circulating CD271+ Mesenchymal Progenitors from Hematopoietic Progenitors During Aging and After Myocardial Infarction

Abstract

The specific cell surface markers on mesenchymal stem/progenitor cells (MSCs) have been poorly defined in vivo, but in one recent study, an MSC subpopulation was directly isolated from a CD271-positive fraction of human bone marrow cells. The aim of this study was to identify circulating CD271(+) MSCs in human peripheral blood and investigate whether the cells are mobilized after acute myocardial infarction (MI). A flow cytometric analysis identified CD45(low/-)CD34(+)CD271(+) cells in adult human peripheral blood. The numbers of circulating CD45(low/-)CD34(+)CD133(+) cells (hematopoietic linage progenitors) were significantly lower in elderly subjects without coronary artery disease than in healthy young subjects, whereas the numbers of CD45(low/-)CD34(+)CD271(+) cells were comparable between elderly subjects and younger subjects. The CD45(low/-)CD34(+)CD271(+) and CD133(+) cell counts were both higher in patients with acute MI than in patients with stable coronary artery disease. In our investigation of the time course changes after acute MI, the CD45(low/-)CD34(+)CD133(+) cell counts gradually increased up to day 7. Over the same period, the CD45(low/-)CD34(+)CD271(+) cell counts peaked at day 3 and then declined up to day 7. Importantly, the CD271(+) cell counts at day 3 were positively correlated with the peak concentrations of creatine kinase after acute MI. Results of the present study suggest that the CD271(+) MSCs are mobilized differently from the CD133(+) hematopoietic progenitors and may play a specific role in the tissue repair process during age-related changes and after acute myocardial infarction.

Aging Effects on Cardiac Progenitor Cell Physiology

“…In a mouse model of accelerated aging due to telomerase deficiency, epidermal stem cells are not able to egress from the niches and accumulate in the hair follicle (106). In the older adults, a suppressed mobilization of HSCs into the peripheral circulation has been reported (102,148). In the MSC compartment of the old bone marrow there is a diminished expression of the genes coding for chemokine and cytokine receptors, which potentially interferes with cell activation and migration (55).…”

Kisspeptin-10 induces endothelial cellular senescence and impaired endothelial cell growth

“…Immunostaining Immunohistochemistry was performed as described previously [18]. Tissue sections were incubated with a specific primary antibody against CD31 at a 1:100 dilution (Dako) overnight at 4 • C, washed three times in PBS and incubated with HRP (horseradish peroxidase)-labelled anti-rabbit or anti-mouse antibody (Histofine Simplestain Max PO; Nichirei).…”

Pharmacological tools to mobilise mesenchymal stromal cells into the blood promote bone formation after surgery

“…Thus, an increase in circulating MSCs has been reported in animal models of injury, and high-mobility group protein B1 (HMGB1) and substance P have been identified as the factors that mediate this response. [3][4][5][6] Furthermore, an increase in circulating stromal cells has been reported in blood from patients following myocardial infarction 7,8 or burns. 9 In theory, increasing circulating numbers of MSCs would increase numbers that could be recruited to sites of tissue injury and thus accelerate or enhance tissue regeneration, as has been shown in the context of skin wound healing 3,10 and bone fractures.…”