Distinct kinetics of subunit autolysis in mammalian m‐calpain activation

doi:10.1016/0014-5793(94)00487-0

Cited by 60 publications

(2 citation statements)

References 17 publications

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“…The second control and ficin treated with inhibitors after storage at 37 °C for 40 h were separately injected into the cation exchange column on HPLC (Shimadzu system with SP-Sepharose column), and changes were separately evaluated. The percentage of autolysis was calculated according to the below formula: %autolysis = [(A 1 − A 2 )/A 1 ] × 100, where A 1 and A 2 are the peaks area of the pre- and post-autolysis forms of the enzyme, respectively [24,29]. …”

Section: Methodsmentioning

confidence: 99%

Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors

Zare

Moosavi-Movahedi

Salami

et al. 2016

International Journal of Biological Macromolecules

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The fig’s ficin is a cysteine endoproteolytic enzyme, which plays fundamental roles in many plant physiological processes, and has many applications in different industries such as pharmaceutical and food. In this work, we report the inhibition and activation of autolysis and structural changes associated with reaction of ficin with iodoacetamide and tetrathionate using high-performance liquid chromatography (HPLC), ultra filtration membrane, and dynamic light scattering (DLS) methods. The ficin structural changes were also determined using UV-absorption, circular dichroism (CD), fluorescence spectroscopy, and differential scanning calorimetry (DSC) techniques. These techniques demonstrated that iodoacetamide completely inhibited ficin autolysis, which was irreversible. However, tetrathionate partially and reversibility inhibited its autolysis. The ficin structural changes with two synthetic inhibitors were associated with secondary structural changes related to decreased alpha-helix and increased beta sheet and random coil conformations, contributing to its aggregation.

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Section: Methodsmentioning

confidence: 99%

Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors

Zare

Moosavi-Movahedi

Salami

et al. 2016

International Journal of Biological Macromolecules

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“…Calpains ( CAPN ) encode intracellular calcium-activated cysteine proteases that have been involved in several physiological and pathological processes [2]. Calpains are regulated by a variety of factors, including a 30-kDa small subunit [3], calcium and phospholipids [4], and calpastatin, a widely distributed calpain-specific inhibitor [5] and [6]. In living muscle, calpains are responsible for remodeling proteins that maintain the structure of skeletal muscle (myofibrillar linkage proteins, MLP) such as titin, nebulin and desmin [7].…”

Section: Introductionmentioning

confidence: 99%

Association between single nucleotide polymorphism in ovine Calpain gene and growth performance in three Egyptian sheep breeds

Mahrous

Hassanane

Shafey

et al. 2016

Journal of Genetic Engineering and Biotechnology

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The aim of the present study was to assess the association of single nucleotide polymorphisms (SNPs) of Calpain (CAPN) gene with birth weight (BW), final weight (FW) and average daily gain (ADG) in three Egyptian sheep breeds: Barki, Rahmani and Ossimi. Blood samples were collected from 108 animals representing the three breeds. DNA was isolated using salting out procedure and then the quality and quantity of DNA extracted were measured. A 190 bp of CAPN was amplified by PCR using specific primers. The allele and genotype frequencies for all the identified SNPs were calculated. The PCR products corresponding to each genotype were sequenced to identify SNPs associated with the traits in question. Two SNPs (C→T) were detected in the nucleotides 44 and 154. For each SNP, the two mentioned alleles were named C and T, respectively. The sequenced CAPN segments were subjected to nucleotide blast at NCBI, which revealed 99% identity with that reported for sheep in Genbank. The TT was the least common genotype, whereas frequencies of CT and CC genotypes were fluctuated in the three sheep breeds under study. Animal carrier TT genotype had higher BW, FW and ADG than those with CT genotype, while the lowest values were associated with CC genotype. For the three traits under study, Rahmani had the highest estimates followed by Ossimi and Barki. Males exhibited heavier BW and FW as well as higher ADG compared with females. The results generated provide preliminary indication of the functional diversity present in Barki, Rahmani and Ossimi sheep and the possibility of using this polymorphism in Egyptian sheep genetic improvement.

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Autolysis and biochemical properties of a lobster muscle calpain-like proteinase

Beyette¹,

Mykles²

1997

J. Exp. Zool.

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Distinct kinetics of subunit autolysis in mammalian m‐calpain activation

Cited by 60 publications

References 17 publications

Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors

Autolysis control and structural changes of purified ficin from Iranian fig latex with synthetic inhibitors

Association between single nucleotide polymorphism in ovine Calpain gene and growth performance in three Egyptian sheep breeds

Autolysis and biochemical properties of a lobster muscle calpain-like proteinase

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