Distinct ipRGC subpopulations mediate light’s acute and circadian effects on body temperature and sleep

Rupp, Alan C.; Ren, Michelle; Altimus, Cara M.; Fernandez, Diego C.; Richardson, Melissa; Turek, Fred W.; Hattar, Samer; Schmidt, Tiffany M.

doi:10.7554/elife.44358

Cited by 87 publications

(71 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in line with data in mice which demonstrated that sustained neuronal activation induced by active exploration in awake animals leads to an increase in SWA during subsequent sleep [29]. [34]. This corroborates how important the role of the daily cycle of light intensity is in shaping temporal sleep-activity patterns independent of circadian photoentrainment but directly via current ecological and physiological settings [35].…”

Section: Resultssupporting

confidence: 88%

Evidence that Homeostatic Sleep Regulation Depends on Ambient Illuminance Levels

Cajochen¹,

Reichert²,

Maire³

et al. 2019

Preprint

View full text Add to dashboard Cite

We examined whether the ambient illuminance during extended wakefulness modulates the homeostatic increase in human deep sleep [i.e. slow wave sleep (SWS) and electroencephalographic (EEG) slow-wave activity (SWA)] in healthy young and older volunteers. Thirty-eight young and older participants underwent 40 hours of extended wakefulness [i.e. sleep deprivation (SD)] once under dim light (DL: 8 lux, 2800K), and once under either white light (WL: 250 lux, 2800K) or blue-enriched white light (BL: 250 lux, 9000K) exposure. Subjective sleepiness was assessed hourly and polysomnography was quantified during the baseline night prior to the 40-h SD and during the subsequent recovery night. Both the young and older participants responded with a higher homeostatic sleep response to 40-h SD after WL and BL than after DL. This was indexed by a significantly faster intra-night accumulation of SWS and a significantly higher response in relative EEG SWA during the recovery night after WL and BL than after DL for both age groups. No significant differences were observed between the WL and BL condition for these two particular SWS and SWA measures. Subjective sleepiness ratings during the 40-h SD were significantly reduced under both WL and BL compared to DL, but were not significantly associated with markers of sleep homeostasis in both age groups. Our data indicate that not only the duration of prior wakefulness, but also the experienced illuminance during wakefulness affects homeostatic sleep regulation in humans. Thus, working extended hours under low illuminance may negatively impact subsequent sleep intensity in humans.

show abstract

Section: Resultssupporting

confidence: 88%

Evidence that Homeostatic Sleep Regulation Depends on Ambient Illuminance Levels

Cajochen¹,

Reichert²,

Maire³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…However, how different wavelengths of light activate specific pathways for arousal promotion in humans remains elusive. Interestingly, it has been recently discovered that distinct ipRGC subpopulations mediate light's acute effect on sleep through a circuitry distinct from that of circadian photoentrainment [34]. This corroborates how important the role of the daily cycle of light intensity is in shaping temporal sleep-activity patterns independent of circadian photoentrainment but directly via current ecological and physiological settings [35].…”

Section: Figurementioning

confidence: 73%

Evidence That Homeostatic Sleep Regulation Depends on Ambient Lighting Conditions during Wakefulness

et al. 2019

View full text Add to dashboard Cite

We examined whether ambient lighting conditions during extended wakefulness modulate the homeostatic response to sleep loss as indexed by. slow wave sleep (SWS) and electroencephalographic (EEG) slow-wave activity (SWA) in healthy young and older volunteers. Thirty-eight young and older participants underwent 40 hours of extended wakefulness [i.e., sleep deprivation (SD)] once under dim light (DL: 8 lux, 2800 K), and once under either white light (WL: 250 lux, 2800 K) or blue-enriched white light (BL: 250 lux, 9000 K) exposure. Subjective sleepiness was assessed hourly and polysomnography was quantified during the baseline night prior to the 40-h SD and during the subsequent recovery night. Both the young and older participants responded with a higher homeostatic sleep response to 40-h SD after WL and BL than after DL. This was indexed by a significantly faster intra-night accumulation of SWS and a significantly higher response in relative EEG SWA during the recovery night after WL and BL than after DL for both age groups. No significant differences were observed between the WL and BL condition for these two particular SWS and SWA measures. Subjective sleepiness ratings during the 40-h SD were significantly reduced under both WL and BL compared to DL, but were not significantly associated with markers of sleep homeostasis in both age groups. Our data indicate that not only the duration of prior wakefulness, but also the experienced illuminance during wakefulness affects homeostatic sleep regulation in humans. Thus, working extended hours under low illuminance may negatively impact subsequent sleep intensity in humans.

show abstract

“…IpRGCs represent a small population of the total cells in the ganglion cell layer. These cells are the primary regulators of non-image forming processes such as light-induced pupil constriction, circadian photoentrainment, and sleep [4,2]. Based on sequence homology, melanopsin is more closely related to visual pigments found in rhabdomeric photoreceptors found in organisms such as Drosophila [5,1].…”

Section: Introductionmentioning

confidence: 99%

Melanopsin Carboxy-terminus phosphorylation plasticity and bulk negative charge, not strict site specificity, achieves phototransduction deactivation

et al. 2020

View full text Add to dashboard Cite

Melanopsin is a visual pigment expressed in a small subset of ganglion cells in the mammalian retina known as intrinsically photosensitive retinal ganglion cells (ipRGCs) and is implicated in regulating non-image forming functions such as circadian photoentrainment and pupil constriction and contrast sensitivity in image formation. Mouse melanopsin's Carboxyterminus (C-terminus) possesses 38 serine and threonine residues, which can potentially serve as phosphorylation sites for a G-protein Receptor Kinase (GRK) and be involved in the deactivation of signal transduction. Previous studies suggest that S388, T389, S391, S392, S394, S395 on the proximal region of the C-terminus of mouse melanopsin are necessary for melanopsin deactivation. We expressed a series of mouse melanopsin C-terminal mutants in HEK293 cells and using calcium imaging, and we found that the necessary cluster of six serine and threonine residues, while being critical, are insufficient for proper melanopsin deactivation. Interestingly, the additional six serine and threonine residues adjacent to the required six sites, in either proximal or distal direction, are capable of restoring wild-type deactivation of melanopsin. These findings suggest an element of plasticity in the molecular basis of melanopsin phosphorylation and deactivation. In addition, C-terminal chimeric mutants and molecular modeling studies support the idea that the initial steps of deactivation and β-arrestin binding are centered around these critical phosphorylation sites (S388-S395). The degree of functional versatility described in this study, along with ipRGC biophysical heterogeneity and the possible use of multiple signal transduction cascades, might contribute to the diverse ipRGC light responses for use in non-image and image forming behaviors, even though all six sub types of ipRGCs express the same melanopsin gene OPN4.

show abstract

Distinct ipRGC subpopulations mediate light’s acute and circadian effects on body temperature and sleep

Cited by 87 publications

References 50 publications

Evidence that Homeostatic Sleep Regulation Depends on Ambient Illuminance Levels

Evidence that Homeostatic Sleep Regulation Depends on Ambient Illuminance Levels

Evidence That Homeostatic Sleep Regulation Depends on Ambient Lighting Conditions during Wakefulness

Melanopsin Carboxy-terminus phosphorylation plasticity and bulk negative charge, not strict site specificity, achieves phototransduction deactivation

Contact Info

Product

Resources

About