Distinct growth stages controlled by the interplay of deterministic and stochastic processes in functional anammox biofilms

Niederdorfer, Robert; Fragner, Lisa; Yuan, Ling; Hausherr, Damian; Wei, Jing; Magyar, Paul; Joss, Adriano; Lehmann, Moritz F.; Ju, Feng; Bürgmann, Helmut

doi:10.1016/j.watres.2021.117225

Cited by 46 publications

(18 citation statements)

References 78 publications

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“…In addition, biofilms on the carrier and the glass were influenced by different small-scale hydraulics, which, in turn, impacted the microbial structure and biodiversity (Niederdorfer et al, 2016). Obviously, the supply of ammonium and DO is higher in biofilms on the walls of the reactor than on the carrier; on the latter, substrate gradient and anaerobic zones exist [the stages of biofilm formation and stratification on the carrier are well described in Niederdorfer et al (2021)]. At the same time, a better DO supply in biofilms on the walls of the reactor creates more favorable conditions for the development of stage I nitrifiers, and, accordingly, anammox bacteria on the glass are provided with nitrite better than in the biofilms on the carrier.…”

Section: Discussionmentioning

confidence: 99%

“…Most studies revealed a reversible inhibition of anammox bacteria by oxygen, and the inhibitory DO concentration varied over a wide range [reviewed by Seuntjens et al (2018) and Cho et al (2020)]. A complete inhibition of anammox activity both at DO < 0.12 mg/l (Strous et al, 1997;Egli et al, 2001;Oshiki et al, 2016;Seuntjens et al, 2018) and at much higher concentrations (1.0 mg/l) (Niederdorfer et al, 2021), as well as retention of activity at DO of 3-3.8 mg/l, was shown for communities enriched with Ca. "Brocadia" (Oshiki et al, 2011;Carvajal-Arroyo et al, 2013;Zekker et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

et al. 2022

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The sensitivity of anaerobic ammonium-oxidizing (anammox) bacteria to environmental fluctuations is a frequent cause of reactor malfunctions. It was hypothesized that the addition of formate and folate would have a stimulating effect on anammox bacteria, which in turn would lead to the stability of the anammox process under conditions of a sharp increase in ammonium load, i.e., it helps overcome a stress factor. The effect of formate and folate was investigated using a setup consisting of three parallel sequencing batch reactors equipped with a carrier. Two runs of the reactors were performed. The composition of the microbial community was studied by the 16S rRNA gene profiling and metagenomic analysis. Among anammox bacteria, Ca. “Brocadia” spp. dominated during the first run. A stimulatory effect of folate on the daily nitrogen removal rate (dN) was identified. The addition of formate led to progress in dissimilatory nitrate reduction and stimulated the growth of Ca. “Jettenia” spp. The spatial separation of two anammox species was observed in the formate reactor: Ca. “Brocadia” occupied the carrier and Ca. “Jettenia”—the walls of the reactors. Biomass storage at low temperature without feeding led to an interspecies shift in anammox bacteria in favor of Ca. “Jettenia.” During the second run, a domination of Ca. “Jettenia” spp. was recorded along with a stimulating effect of formate, and there was no effect of folate on dN. A comparative genome analysis revealed the patterns suggesting different strategies used by Ca. “Brocadia” and Ca. “Jettenia” spp. to cope with environmental changes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

et al. 2022

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“…For example, previous studies found that the extraordinary succession period of anammox biofilms depended on the habitat created by initial colonization of heterotrophic bacteria. 66 In addition, the carrier material was an important abiotic selection force, which could select distinctive pioneers and further exert a distinct priority effect on subsequent successors, as indicated by the distinct microbial community on different pipe walls. 73 Further research on this discrepancy could provide new insights into the modification and application of various carrier materials.…”

Section: ■ Discussionmentioning

confidence: 99%

“…In this study, the fundamental ecological processes underpinning the development-to-function linkages were proposed to better understand the biofilm-related engineered ecosystems. Even in the well-controlled bioreactor, distinct phase characteristics were driven by internal ecological processes, , resulting in a distinctive core community at distinct biofilm development phases (Figure ). In the engineered system, biofilm models were regarded as essential tools for fundamental biofilm research and the reactor design .…”

Section: Discussionmentioning

confidence: 99%

“…However, the exact development process of specific functional biofilms might be distinct. For example, previous studies found that the extraordinary succession period of anammox biofilms depended on the habitat created by initial colonization of heterotrophic bacteria . In addition, the carrier material was an important abiotic selection force, which could select distinctive pioneers and further exert a distinct priority effect on subsequent successors, as indicated by the distinct microbial community on different pipe walls .…”

Section: Discussionmentioning

confidence: 99%

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Ecological Linkages between a Biofilm Ecosystem and Reactor Performance: The Specificity of Biofilm Development Phases

Yuan

Wang

et al. 2021

Environ. Sci. Technol.

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In biofilm-based engineered ecosystems, the reactor performance was closely linked to interspecies interactions within a biofilm ecosystem, whereas the ecological processes underpinning such linkage were still unenlightened. Herein, the principles of community succession and assembly were integrated to capture the ecological laws of biofilm development by molecular ecological networks and assembly model analysis based on the 16S rRNA sequencing analysis and metagenomics in a well-controlled moving bed biofilm reactor. At the initial colonization phase (days 0−2, driven by initial colonizers), interspecific cooperation (74.18%) facilitated initial biofilm formation, whereas some pioneers, and keystone species disappeared at later phases. At the accumulation phase (days 3−30, rapid biofilm development), interspecific cooperation (81.41 ± 5.07%) contributed to rapid biofilm development and keystone species were mainly involved in quorum sensing or positively correlated with extracellular polymeric substance production. At the maturation phase (days 31−106, a well-adapted quasi-equilibrium state), increased interspecific competition (32.74 ± 4.77%) and higher small-world property facilitated the rapid information transportation and pollutant treatment, and keystone species were positively correlated with the removal of COD and NH 4 + -N. Homogenizing dispersal diminished the contemporary community dissimilarities, while turnover but rather nestedness governed the temporal variations in the biofilm succession period. This study highlighted the specificity of ecological processes at distinct biofilm development phases, which would advance our understanding on the development-to-function linkages in biofilm-based treatment processes.

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Time‐lagged interspecies interactions prevail during biofilm development in moving bed biofilm reactor

Yuan

Guo

Huang

et al. 2022

Biotech & Bioengineering

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Clarifying the essential succession dynamics of interspecies interactions during biofilm development is crucial for the regulation and application of biofilm-based processes. In this study, regular and time-series phylogenetic molecular ecological networks were constructed to investigate ordinary and time-lagged interspecies interactions during biofilm development in a moving bed biofilm reactor. Positive interactions dominated both regular (89.78%) and time-series (77.04%) ecological networks, suggesting that extensive cooperative behaviors facilitated biofilm development. The pronounced directional interactions (72.52%) in the time-series network further indicated that time-lagged interspecies interactions prevailed in the biofilm development process. Specifically, the proportion of directional negative interactions was higher than that of positive interactions, implying that interspecific competition preferred to be time-lagged. The time-series network revealed that module hubs exhibited extensive time-lagged positive interactions with their neighbors, and most of them exhibited altruistic behaviors. Keystone species possessing more positive interactions were positively correlated with biofilm biomass, NO 3 − -N concentrations, and the removal efficiencies of NH 4 + -N and chemical oxygen demand. However, keystone species and peripherals that were negatively targeted by their neighbors showed positive correlations with the concentrations of NO 2 − -N, polysaccharides, and proteins in the soluble microbial products. The data highlight that the time-series network can provide directional microbial interactions along with the biofilm development process, which would help to predict the tendency of community shifts and propose efficient strategies for the regulation of biofilm-based processes.

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Distinct growth stages controlled by the interplay of deterministic and stochastic processes in functional anammox biofilms

Cited by 46 publications

References 78 publications

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

New Insight Into the Interspecies Shift of Anammox Bacteria Ca. “Brocadia” and Ca. “Jettenia” in Reactors Fed With Formate and Folate

Ecological Linkages between a Biofilm Ecosystem and Reactor Performance: The Specificity of Biofilm Development Phases

Time‐lagged interspecies interactions prevail during biofilm development in moving bed biofilm reactor

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