Distinct Expression Profiles and Different Functions of Odorant Binding Proteins in Nilaparvata lugens Stål

He, Pinjing; Zhang, Jin; Liu, Nai-Yong; Zhang, Yanan; Yang, Ke; Dong, Shuang-Lin

doi:10.1371/journal.pone.0028921

Cited by 122 publications

(142 citation statements)

References 52 publications

(61 reference statements)

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“…In the brown planthopper Nilaparvata lugens , for example, dietary introduction of dsRNAs can not only effectively silence target genes that are mainly expressed in gut [8]–[10], or widely expressed in various tissues [11], [12], but also knock down target genes which are expressed in other tissues far from the midgut [13]. Similar results have been documented in S. furcifera and L. striatellus [14]–[16].…”

Section: Introductionsupporting

confidence: 69%

RNA Interference Depletion of the Halloween Gene Disembodied Implies its Potential Application for Management of Planthopper Sogatella furcifera and Laodelphax striatellus

Wan

Song

et al. 2014

PLoS ONE

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Sogatella furcifera and Laodelphax striatellus are economically important rice pests in China by acting as vectors of several rice viruses, sucking the phloem sap and blocking the phloem vessels. Ecdysteroid hormone 20-hydroxyecdysone regulates insect development and reproduction. A cytochrome P450 monooxygenase CYP302A1 (22-hydroxylase), encoded by the Halloween gene disembodied (dib), plays a critical role in ecdysteroidogenesis. The objective of this study is to test whether dib genes are potential targets for RNA interference-based management of S. furcifera and L. striatellus. We cloned and characterized Sfdib and Lsdib. The open reading frame regions of dib genes were generated and used for designing and constructing dsRNA fragments. Experiments were conducted using oral delivery of dsdib to investigate the effectiveness of RNAi in S. furcifera and L. striatellus nymphs. Real-time quantitative reverse transcriptase-PCR analysis demonstrated that continuous ingestion of dsdib at the concentration of 0.01, 0.05 and 0.50 mg/ml diminished Sfdib expression levels by 35.9%, 45.1% and 66.2%, and ecdysone receptor (SfEcR) gene mRNA levels by 34.0%, 36.2% and 58.5% respectively in S. furcifera, and decreased Lsdib expression level by 18.8%, 35.8% and 56.7%, and LsEcR mRNA levels by 25.2%, 46.8% and 68.8% respectively in L. striatellus. The reduction in dib and EcR transcript abundance resulted in observable phenotypes. The development of nymphs was impaired and the survival was negatively affected. Our data will enable the development of new insect control strategies and functional analysis of vital genes in S. furcifera and L. striatellus nymphs.

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Section: Introductionsupporting

confidence: 69%

RNA Interference Depletion of the Halloween Gene Disembodied Implies its Potential Application for Management of Planthopper Sogatella furcifera and Laodelphax striatellus

Wan

Song

et al. 2014

PLoS ONE

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“…The function of OBPs in coleopteran insect communication is starting to be unraveled, with several OBPs having been isolated in moths, dipterans and hemipterans and displaying affinities to host plant volatile compounds or pheromones [5]–[7]. As their name implies, OBPs uptake volatile odors that enter the sensillum pore, bind physiologically relevant molecules and transport their cargo to the surface of ORNs [8].…”

Section: Introductionmentioning

confidence: 99%

Identification of Odor-Processing Genes in the Emerald Ash Borer, Agrilus planipennis

Mamidala

Wijeratne

et al. 2013

PLoS ONE

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BackgroundInsects rely on olfaction to locate food, mates, and suitable oviposition sites for successful completion of their life cycle. Agrilus planipennis Fairmaire (emerald ash borer) is a serious invasive insect pest that has killed tens of millions of North American ash (Fraxinus spp) trees and threatens the very existence of the genus Fraxinus. Adult A. planipennis are attracted to host volatiles and conspecifics; however, to date no molecular knowledge exists on olfaction in A. planipennis. Hence, we undertook an antennae-specific transcriptomic study to identify the repertoire of odor processing genes involved in A. planipennis olfaction.Methodology and Principal FindingsWe acquired 139,085 Roche/454 GS FLX transcriptomic reads that were assembled into 30,615 high quality expressed sequence tags (ESTs), including 3,249 isotigs and 27,366 non-isotigs (contigs and singletons). Intriguingly, the majority of the A. planipennis antennal transcripts (59.72%) did not show similarity with sequences deposited in the non-redundant database of GenBank, potentially representing novel genes. Functional annotation and KEGG analysis revealed pathways associated with signaling and detoxification. Several odor processing genes (9 odorant binding proteins, 2 odorant receptors, 1 sensory neuron membrane protein and 134 odorant/xenobiotic degradation enzymes, including cytochrome P450s, glutathione-S-transferases; esterases, etc.) putatively involved in olfaction processes were identified. Quantitative PCR of candidate genes in male and female A. planipennis in different developmental stages revealed developmental- and sex-biased expression patterns.Conclusions and SignificanceThe antennal ESTs derived from A. planipennis constitute a rich molecular resource for the identification of genes potentially involved in the olfaction process of A. planipennis. These findings should help in understanding the processing of antennally-active compounds (e.g. 7-epi-sesquithujene) previously identified in this serious invasive pest.

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“…Oral delivery of dsRNA has enormous potential applications in the management of agricultural insect pests. Diet-mediated dsRNA-induced gene silencing has been previously evidenced in a wide variety of agricultural insect pests of the order Lepidoptera [46][47][48][49], Diptera [13,[50][51][52][53], Orthoptera [54], Hymenoptera [55], Hemiptera [15,[56][57][58][59][60][61][62][63][64][65], Coleoptera [63,[66][67][68][69], and Isoptera [16]. So far, dsRNA delivery to various insect has been accomplished through oral feeding, microinjection, soaking, and transgenics [12,70,71,46].…”

Section: Discussionmentioning

confidence: 99%

Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

Asokan

Rebijith

Roopa

et al. 2014

Appl Biochem Biotechnol

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The sweet potato whitefly, Bemisia tabaci (G.) biotype B (Hemiptera: Aleyrodidae), is one of the most economically important pest, by being a dreaded vector of Geminiviruses, and also causes direct damage to the crops by sucking phloem sap. Glutathione S-transferase (GST) is a large family of multifunctional enzymes that play pivotal roles in the detoxification of secondary allelochemical produced by the host plants and in insecticide resistance, thus regulates insect growth and development. The objective of this study is to show the potential of RNA interference (RNAi) in the management of B. tabaci. RNAi is a sequence-specific gene silencing mechanism induced by double-stranded RNA (dsRNA) which holds tremendous potential in pest management. In this regard, we sequenced the GST from B. tabaci and synthesized approximately 500-bp dsRNA from the above and delivered through diet to B. tabaci. Real-time quantitative PCR (RT-qPCR) showed that continuous application of dsGST at 1.0, 0.5, and 0.25 μg/μl reduced mRNA expression levels for BtGST by 77.43, 64.86, and 52.95 % which resulted in mortality by 77, 59, and 40 %, respectively, after 72 h of application. Disruption of BtGST expression will enable the development of novel strategies in pest management and functional analysis of vital genes in B. tabaci.

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Distinct Expression Profiles and Different Functions of Odorant Binding Proteins in Nilaparvata lugens Stål

Cited by 122 publications

References 52 publications

RNA Interference Depletion of the Halloween Gene Disembodied Implies its Potential Application for Management of Planthopper Sogatella furcifera and Laodelphax striatellus

RNA Interference Depletion of the Halloween Gene Disembodied Implies its Potential Application for Management of Planthopper Sogatella furcifera and Laodelphax striatellus

Identification of Odor-Processing Genes in the Emerald Ash Borer, Agrilus planipennis

Non-Invasive Delivery of dsGST Is Lethal to the Sweet Potato Whitefly, Bemisia tabaci (G.) (Hemiptera: Aleyrodidae)

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