Distinct enhancer-promoter modes determine Sox2 regulation in mouse pluripotent cells

Exploring the Role of Enhancer-Mediated Transcriptional Regulation in Precision Biology

“…Additionally, an enhancer may interact with multiple target genes, and determining the true targets of an enhancer presents a challenge. Furthermore, different enhancers may collaborate to regulate a gene [ 26 , 56 ], and the regulatory mechanism underlying this collaboration is not fully understood. The multi-enhancer regulatory model we propose may provide a framework to explain this regulatory mechanism, and we hope that future researchers will conduct more in-depth studies in this direction.…”

“…The question of how Sox2 controls the direction of cell differentiation is of particular interest. Our recent study revealed different transcriptional regulation patterns of the Sox2 gene associated with distinct enhancer-promoter interactions [ 11 , 26 ] ( Figure 4 ). In a previous study, we identified three enhancers that interact with the Sox2 promoter, each playing a unique role [ 11 ].…”

“…We found that these three enhancers affect various physiological processes in stem cell differentiation, including differentiation into cardiomyocytes, early differentiation of mESCs into neuronal cells, early embryonic differentiation, and stem cell morphology maintenance. These findings suggest that different enhancers exhibit diverse transcriptional profiles [ 26 ] ( Figure 4 ), highlighting the complex E-P association. This complex association, which synergistically regulates Sox2 gene function in multiple ways, provides a new paradigm for the precise study of gene function and forms the basis for precision biology research.…”

“…Herein, we summarize the research progress, focusing on the importance of enhancer-mediated transcriptional regulation in achieving precision biology. Specifically, we highlight the multi-enhancer transcriptional regulation model, which offers a new approach to studying the function and molecular mechanism of individual genes [ 26 ]. We hope to provide novel insights into the study of multi-enhancer regulatory models, enhancing our understanding of the mechanisms involved in integrating information through complex devices in transcriptional regulation.…”