Distinct dual enzyme-like activities of Fe–N–C single-atom nanozymes enable discriminative detection of cellular glutathione

Abstract

Discriminative detection of glutathione was readily achieved by amplifying the difference of dual enzyme-like activities of Fe–N–C single-atom nanozymes.

Novel Immunosensor Based on Metal Single-Atom Nanozymes with Enhanced Oxidase-Like Activity for Capsaicin Analysis in Spicy Food

“…Scavengers, including propan-2-ol (IPA, • OH), nitrotetrazolium blue chloride (NBT, • O 2 − ), and tryptophan (Try, 1 O 2 ), were adopted to imply the species of reactive oxygen produced by Fe 0.7 /NC. 43 We observed that the OXD-like activity of Fe 0.7 /NC was significantly inhibited by NBT, indicating that • O 2 − was exclusively generated in the OXD-like catalytic system (Figure 3H). Furthermore, electron paramagnetic resonance (EPR) spectroscopy with 2,6-dimethylpyridine N-oxide as a pintrapping agent was used to confirm the • O 2 − generation (Figure 3I).…”

“…This suggested that atomically dispersed Fe moieties acted as primary active sites during the OXD-like activity. Scavengers, including propan-2-ol (IPA, • OH), nitrotetrazolium blue chloride (NBT, • O 2 – ), and tryptophan (Try, 1 O 2 ), were adopted to imply the species of reactive oxygen produced by Fe 0.7 /NC . We observed that the OXD-like activity of Fe 0.7 /NC was significantly inhibited by NBT, indicating that • O 2 – was exclusively generated in the OXD-like catalytic system (Figure H).…”

Molecular Network-Bearing Fe–N–C Single-Atom Nanozymes for Monitoring Intracellular Glutathione Fluctuations

“…Fe–N–C was prepared according to the previous report . Typically, Zn­(NO 3 ) 2 ·6H 2 O (1041 mg, 3.5 mmol) and Fe­(acac) 3 (90 mg, 0.25 mmol) were dispersed in methanol (20 mL) under ultrasonication.…”

“…Its abnormal level is often associated with various diseases such as cancer, liver damage, and neurodegenerative disease. − So it is of great significance to accurately determine the GSH content. Although some nanozyme-based colorimetric sensors have been established for this purpose (Table S1), − it is still difficult for those bare nanozymes to efficiently differentiate GSH from other two common biothiols, cysteine (Cys) and homocysteine (Hcy), due to their similar structure and activity. , Herein, based on the presented surface engineering strategy, a molecular network containing disulfide bonds was woven on the Fe–N–C surface by a sequential modification process (Scheme ). Specifically, Fe–N–C was prepared through a high-temperature calcination approach with an Fe(acac) 3 -incorporated zeolitic imidazolate framework-8 (Fe/ZIF-8) as the precursor.…”

“…Fe−N−C was prepared according to the previous report. 51 Typically, Zn(NO 3 ) 2 •6H 2 O (1041 mg, 3.5 mmol) and Fe(acac) 3 (90 mg, 0.25 mmol) were dispersed in methanol (20 mL) under ultrasonication. 2-Methylimidazole (1150 mg, 14 mmol) was added into a flask containing methanol (10 mL) upon stirring.…”