Distinct Dominant Lineage from In Vitro Expanded Adipose-Derived Stem Cells (ASCs) Exhibits Enhanced Wound Healing Properties

Abstract

It has been suggested that immunophenotypically defined lineages within the in vitro expanded adipose-derived stem cell (ASC) may play a beneficial role from the perspective of a personalized intervention. Therefore, to better understand the implications of different surface marker profiles for the functionality, we set out to examine the evolution of ASC-variants based on the co-expression of five bright or eight dim epitopes. At passages P1, P4, and P8, the co-localization of five bright markers (CD73, CD90, CD105, CD166, and CD201), or eight dim markers (CD34, CD36, CD200, CD248, CD271, CD274, CD146, and the Stro-1), was investigated by flow cytometry. Selected subpopulations were isolated using the fluorescence-activated cells sorting from the cryopreserved P4 and analyzed in terms of proliferative and clonogenic properties, trilineage differentiation, and wound healing potential. Only two of the dim epitopes were found in representative subpopulations (SP), and from the P4 onwards, two major combinations featuring the CD274+ (SP1) or the CD274+ CD146+ (SP2) emerged. Upon sorting and growth, both subpopulations assumed new but highly similar clonal profiles, consisting of the CD274+ CD146+ and the CD274+ CD146+ CD248+ phenotypes. The functional analysis revealed that the SP2 surpassed SP1 and the unfractionated cells regarding the growth rate, clonogenic activity, and the wound closure and endothelial tube formation potential. The surface epitopes may be considered a tool to enrich specific functionality and thus improve therapeutic outcomes in dedicated circumstances.

Potency assays for human adipose-derived stem cells as a medicinal product toward wound healing

“…Relevant for the granulation tissue formation by fibroblasts the ASC subpopulation characterized as CD274 + CD146 + was found superior in stimulating fibroblast migration [ 87 ] .…”

Correction: Peng et al. Distinct Dominant Lineage from In Vitro Expanded Adipose-Derived Stem Cells (ASCs) Exhibits Enhanced Wound Healing Properties. Cells 2022, 11, 1236

“…In the original publication [ 1 ]. In Figure 4 A, entitled “proliferation”, the legend colors for SP1 and SP2 have been swapped; blue represents SP1, and red represents SP2.…”

Translation of Adipose-Derived Stem Cells Into Clinical Use to Treat Chronic Wounds

“…To better understand the connotations of different surface marker profiles in the wound healing perspective, we examined in Study 3 the evolution of ASC-variants based on the co-expression of CD markers [189].…”

“…Study 3 indicates that selected clones with enhanced wound-healing potential may exist within the expanded ASC subsets. However, it is still unknown how universally this phenomenon applies to the donor population; therefore, continuation studies are required in this area to promote more efficient and personalized ASC-based therapies [189].…”