Distinct cardioprotective effects of 17β-estradiol and dehydroepiandrosterone on pressure overload–induced hypertrophy in ovariectomized female rats

Abstract

Taken together, these results from an ovariectomized rat model of PO-induced cardiac dysfunction show that DHEA but not E2 elicits a cardioprotective action through σ1 receptor activation. DHEA-induced Akt-eNOS activation through σ1 receptors is probably associated with its cardioprotective activity.

Methyl pyruvate rescues mitochondrial damage caused by SIGMAR1 mutation related to amyotrophic lateral sclerosis

“…After immunoprecipitation with an anti-IP 3 R3 antibody, immunoprecipitants were separated on SDS-PAGE and subjected to Western blot analysis using an anti-DsRed antibody. In immunoblotting analyses to determine protein levels, cultured Neuro2a cells were washed with PBS at 4°C and stored at −80°C until analyses were performed [23]. For assays, frozen Neuro2A cells were homogenized using methods previously described [18].…”

“…Immunoprecipitation and Western blotting were performed using previously described methods [18,23]. Immunoprecipitation analysis of σ 1 R and IP 3 R3 was undertaken using lysates of Neuro2A cells with or without transfection with σ 1 R-mCherry or σ 1 R E102Q -mCherry.…”

Crucial Interactions Between Selective Serotonin Uptake Inhibitors and Sigma-1 Receptor in Heart Failure

“…Although these mechanisms remain unclear, our work suggests that the cardiac sigma-1 receptor and its downstream signaling protect the heart against TAC (22) and PO-induced cardiomyopathy (21,25). Previously, we also reported in several studies that the neurosteroid dehydroepiandrosterone (DHEA) serves as an endogenous ligand for the sigma-1 receptor and that treatment with DHEA ameliorated PO-induced cardiac injury by up-regulating the cardiac sigma-1 receptor in heart and activating downstream signaling (21,26,27). In support of our hypothesis, a recent study demonstrated significant reduction in DHEA-sulfate concentrations in brain circumventricular and hypothalamic tissue after aortic banding (23).…”

Diverse regulation of IP3 and ryanodine receptors by pentazocine through σ1-receptor in cardiomyocytes

“…Cardiac gene expression was analyzed by real-time RT-PCR as described previously (29). Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA) from LV after 4 wk of PO in OVX female rats.…”