Distinct Anaplasma phagocytophilum genotypes associated with Ixodes trianguliceps ticks and rodents in Central Europe

Blaňárová, Lucia; Stanko, Michał; Carpi, Giovanna; Miklisová, Dana; Víchová, Bronislava; Mošanský, Ladislav; Bona, Martin; Derdáková, Markéta

doi:10.1016/j.ttbdis.2014.07.012

Cited by 60 publications

(58 citation statements)

References 57 publications

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“…phagocytophilum (1.1% [7/631]) is found in Apodemus sylvaticus, Myodes glareolus and Apodemus flavicollis species rodents in Germany . Similarly, Blanarová et al (2014) detected An. phagocytophilum in 1.6% (11/669) of ear punch samples and 2.2% (9/407) of spleen samples of Ap.…”

Section: Introductionmentioning

confidence: 92%

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Benevenute

Dumler

Ogrzewalska

et al. 2017

Ticks and Tick-borne Diseases

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New genotypes of Anaplasmataceae agents have been detected in wild carnivores, birds and deer in Brazil. The present work aimed to investigate the presence of Ehrlichia and Anaplasma species in rodents sampled in Brazil. Additionally, a newly designed quantitative 5' nuclease real-time multiplex PCR for Ehrlichia and Anaplasma spp. detection based on groEL gene amplification was designed, showing high specificity and sensitivity (10 groEL fragment copy/μL). Between 2000 and 2011, different rodent species [n=60] were trapped in 5 Brazilian biomes. Among 458 rodent spleen samples, 0.4% (2/458) and 2.4% (11/458) were positive for Ehrlichia and Anaplasma spp., respectively. Of 458 samples, 2.0% (9/458) and 1.1% (5/458) were positive for Anaplasma sp. and Ehrlichia sp., respectively, using conventional 16S rRNA PCR assays. Maximum Likelihood phylogenetic analyse based on a small region of 16S rRNA genes positioned the Anaplasma genotypes in rodents near Anaplasma phagocytophilum or Anaplasma marginale and Anaplasma odocoilei isolates. Ehrlichia genotypes were closely related to E. canis. There was a low occurrence of Anaplasma and Ehrlichia in wild and synanthropic rodents in Brazil, suggesting the circulation of new genotypes of these agents in rodents in the studied areas.

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Section: Introductionmentioning

confidence: 92%

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Benevenute

Dumler

Ogrzewalska

et al. 2017

Ticks and Tick-borne Diseases

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“…Wild boars ( Sus scrofa ) are also suspected to be reservoir hosts for human A. phagocytophilum variants [8, 10]. Finally, several rodent species have been suspected as reservoir hosts for A. phagocytophilum , but - at least at the scale of published studies - they appear to be involved in an epidemiological cycle independent from those involving ruminants, in which rodents are the only mammalian hosts [11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Multiple-locus variable-number tandem repeat analysis potentially reveals the existence of two groups of Anaplasma phagocytophilum circulating in cattle in France with different wild reservoirs

Dugat

Zanella

Véran³

et al. 2016

Parasites Vectors

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Background Anaplasma phagocytophilum is the causative agent of tick-borne fever, a disease with high economic impact for domestic ruminants in Europe. Epidemiological cycles of this species are complex, and involve different ecotypes circulating in various host species. To date, these epidemiological cycles are poorly understood, especially in Europe, as European reservoir hosts (i.e. vertebrate hosts enabling long-term maintenance of the bacterium in the ecosystem), of the bacterium have not yet been clearly identified. In this study, our objective was to explore the presence, the prevalence, and the genetic diversity of A. phagocytophilum in wild animals, in order to better understand their implications as reservoir hosts of this pathogen.MethodsThe spleens of 101 wild animals were collected from central France and tested for the presence of A. phagocytophilum DNA by msp2 qPCR. Positive samples were then typed by multi-locus variable-number tandem repeat (VNTR) analysis (MLVA), and compared to 179 previously typed A. phagocytophilum samples.Results Anaplasma phagocytophilum DNA was detected in 82/101 (81.2%) animals including 48/49 red deer (98%), 20/21 roe deer (95.2%), 13/29 wild boars (44.8%), and 1/1 red fox. MLVA enabled the discrimination of two A. phagocytophilum groups: group A contained the majority of A. phagocytophilum from red deer and two thirds of those from cattle, while group B included a human strain and variants from diverse animal species, i.e. sheep, dogs, a horse, the majority of variants from roe deer, and the remaining variants from cattle and red deer.ConclusionsOur results suggest that red deer and roe deer are promising A. phagocytophilum reservoir host candidates. Moreover, we also showed that A. phagocytophilum potentially circulates in at least two epidemiological cycles in French cattle. The first cycle may involve red deer as reservoir hosts and cattle as accidental hosts for Group A strains, whereas the second cycle could involve roe deer as reservoir hosts and at least domestic ruminants, dogs, horses, and humans as accidental hosts for Group B strains.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1888-4) contains supplementary material, which is available to authorized users.

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“…All developmental stages of I. trianguliceps predominantly parasitize small mammals. Despite the fact that I. trianguliceps ticks do not have any epidemic significance, these ticks can be involved in the circulation of Borrelia afzelii, Borrelia garinii, Babesia microti and Anaplasma phagocytophilum in nature (Korenberg et al, 2002;Nefedova et al, 2005;Bown et al, 2008;Blaň arová et al, 2014;Rar et al, 2014). The question of whether I. trianguliceps is associated with Rickettsia spp.…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of Rickettsia spp. in Ixodes persulcatus/Ixodes trianguliceps sympatric areas from Western Siberia, Russia: Identification of a new Candidatus Rickettsia species

Igolkina

Рар

Yakimenko

et al. 2015

Infection, Genetics and Evolution

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Distinct Anaplasma phagocytophilum genotypes associated with Ixodes trianguliceps ticks and rodents in Central Europe

Cited by 60 publications

References 57 publications

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Assessment of a quantitative 5′ nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil

Multiple-locus variable-number tandem repeat analysis potentially reveals the existence of two groups of Anaplasma phagocytophilum circulating in cattle in France with different wild reservoirs

Genetic variability of Rickettsia spp. in Ixodes persulcatus/Ixodes trianguliceps sympatric areas from Western Siberia, Russia: Identification of a new Candidatus Rickettsia species

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