Distinct Activities of Myf5 and MyoD Indicate Separate Roles in Skeletal Muscle Lineage Specification and Differentiation

Conerly, Melissa; Yao, Zizhen; Zhong, Jun; Groudine, Mark; Tapscott, Stephen J.

doi:10.1016/j.devcel.2016.01.021

Cited by 98 publications

(101 citation statements)

References 46 publications

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“…The myogenic transcriptional activity of MRFs is also not equivalent, as showed by the role of Myogenin and Myf6 in the later stages of myocyte differentiation [17–19], while Myf5 and MyoD play a major role in myoblasts determination [20]. Interestingly, Myf5 and MyoD also differ on their capability to remodel chromatin and recruit RNA polymerase II [21]. The complexity of the skeletal myogenic program is also highlighted by the complex regulatory regions driving the expression of these transcription factors, with the Myf5–Myf6 and MyoD loci representing the most well characterized [22].…”

Section: Skeletal Myogenesis In the Embryomentioning

confidence: 99%

“…The combinatorial composition of subunits within chromatin remodeling complexes of the SWI/SNF family has an important role in the modulation of the activity of these ATP-dependent enzymes (reviewed by [144]), and inclusion of BAF60C in these complexes during gastrulation may be associated with chromatin remodeling of specific sites important for mesodermal patterning. Although there are no genome wide studies validating the function of BAF60C at MYOD sites, studies from the Tapscott lab demonstrated that both Myf5 and MyoD bind similar loci and induce chromatin remodeling through an increase in acetyl-histone 4 (H4Ac) levels [21, 101], but only MyoD binding is associated with recruitment of RNA pol II and robust transcription. In addition, the Dynlacht group showed that MyoD also binds at skeletal muscle enhancers, and that this is associated with maintenance of monomethyl-lysine 4 histone 3 (H3K4me1) levels [145].…”

Section: Requirement Of a Mesodermal Intermediate And Differences mentioning

confidence: 99%

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Myogenic progenitor specification from pluripotent stem cells

Magli

Perlingeiro

2017

Seminars in Cell & Developmental Biology

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Pluripotent stem cells represent important tools for both basic and translational science as they enable to study mechanisms of development, model diseases in vitro and provide a potential source of tissue-specific progenitors for cell therapy. Concomitantly with the increasing knowledge of the molecular mechanisms behind activation of the skeletal myogenic program during embryonic development, novel findings in the stem cell field provided the opportunity to begin recapitulating in vitro the events occurring during specification of the myogenic lineage. In this review, we will provide a perspective of the molecular mechanisms responsible for skeletal myogenic commitment in the embryo and how this knowledge was instrumental for specifying this lineage from pluripotent stem cells. In addition, we will discuss the current limitations for properly recapitulating skeletal myogenesis in the petri dish, and we will provide insights about future applications of pluripotent stem cell-derived myogenic cells.

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Section: Skeletal Myogenesis In the Embryomentioning

confidence: 99%

Section: Requirement Of a Mesodermal Intermediate And Differences mentioning

confidence: 99%

Myogenic progenitor specification from pluripotent stem cells

Magli

Perlingeiro

2017

Seminars in Cell & Developmental Biology

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“…At the earliest stages of myoblast differentiation, promoters controlling the expression of differentiation-specific genes become hyperacetylated in a MyoD- or Myf5-dependent manner (46, 60, 61). This is accomplished by downregulation of Snail, HDACs and Ezh2 levels and the alleviation of repression of acetyltransferase activity (22, 24, 62, 63).…”

Section: The Chromatin State At Active Myogenic Locimentioning

confidence: 99%

Temporal regulation of chromatin during myoblast differentiation

Harada

Ohkawa

2017

Seminars in Cell & Developmental Biology

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The commitment to and execution of differentiation programs involves a significant change in gene expression in the precursor cell to facilitate development of the mature cell type. In addition to being regulated by lineage-determining and auxiliary transcription factors that drive these changes, the structural status of the chromatin has a considerable impact on the transcriptional competence of differentiation-specific genes, which is clearly demonstrated by the large number of cofactors and the extraordinary complex mechanisms by which these genes become activated. The terminal differentiation of myoblasts to myotubes and mature skeletal muscle is an excellent system to illustrate these points. The MyoD family of closely related, lineage-determining transcription factors directs, largely through targeting to chromatin, a cascade of cooperating transcription factors and enzymes that incorporate or remove variant histones, post-translationally modify histones, and alter nucleosome structure and positioning via energy released by ATP hydrolysis. The coordinated action of these transcription factors and enzymes prevents expression of differentiation-specific genes in myoblasts and facilitates the transition of these genes from transcriptionally repressed to activated during the differentiation process. Regulation is achieved in both a temporal as well as spatial manner, as at least some of these factors and enzymes affect local chromatin structure at myogenic gene regulatory sequences as well as higher-order genome organization. Here we discuss the transition of genes that promote myoblast differentiation from the silenced to the activated state with an emphasis on the changes that occur to individual histones and the chromatin structure present at these loci.

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“…High MyoD expression in muscle satellite cells represses cell renewal and promotes terminal differentiation [10]. MyoD family inhibitor ( MDFI ) functions as a negative regulator of muscle growth by inhibiting muscle hypertrophy [11, 12].…”

Section: Introductionmentioning

confidence: 99%

MiR-27b Promotes Muscle Development by Inhibiting MDFI Expression

Hou

Jiao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Skeletal muscle plays an essential role in the body movement. However, injuries to the skeletal muscle are common. Lifelong maintenance of skeletal muscle function largely depends on preserving the regenerative capacity of muscle. Muscle satellite cells proliferation, differentiation, and myoblast fusion play an important role in muscle regeneration after injury. Therefore, understanding of the mechanisms associated with muscle development during muscle regeneration is essential for devising the alternative treatments for muscle injury in the future. Methods: Edu staining, qRT-PCR and western blot were used to evaluate the miR-27b effects on pig muscle satellite cells (PSCs) proliferation and differentiation in vitro. Then, we used bioinformatics analysis and dual-luciferase reporter assay to predict and confirm the miR-27b target gene. Finally, we elucidate the target gene function on muscle development in vitro and in vivo through Edu staining, qRT-PCR, western blot, H&E staining and morphological observation. Result: miR-27b inhibits PSCs proliferation and promotes PSCs differentiation. And the miR-27b target gene, MDFI, promotes PSCs proliferation and inhibits PSCs differentiation in vitro. Furthermore, interfering MDFI expression promotes mice muscle regeneration after injury. Conclusion: our results conclude that miR-27b promotes PSCs myogenesis by targeting MDFI. These results expand our understanding of muscle development mechanism in which miRNAs and genes work collaboratively in regulating skeletal muscle development. Furthermore, this finding has implications for obtaining the alternative treatments for patients with the muscle injury.

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Distinct Activities of Myf5 and MyoD Indicate Separate Roles in Skeletal Muscle Lineage Specification and Differentiation

Cited by 98 publications

References 46 publications

Myogenic progenitor specification from pluripotent stem cells

Myogenic progenitor specification from pluripotent stem cells

Temporal regulation of chromatin during myoblast differentiation

MiR-27b Promotes Muscle Development by Inhibiting MDFI Expression

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