Distinct activation of primary human BDCA1+ dendritic cells upon interaction with stressed or infected β cells

Schulte, Barbara; Kers‐Rebel, Esther D.; Bottino, Rita; Piganelli, Jon D.; Galama, J.M.D.; Engelse, Marten A.; Koning, Eelco J.P. de; Adema, Gosse J.

doi:10.1111/cei.12779

Cited by 2 publications

(3 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EV capsid antigens are expressed in cardiomyocytes of subjects with chronic cardiomyopathy 14, 46, 51, 52 , as well as in pancreatic beta cells 53–55 and lymphoid cells of diabetic patients 29, 56 . Notably, Norwegian investigators succeeded in detecting EV infection in pancreata of type 1 diabetics at the clinical onset 9, 57 .…”

Section: Discussionmentioning

confidence: 99%

“…While neuropathology studies of PPS using either EV antibodies or modern genomics have not yet been reported 50 , studies with EV antibodies have been performed in myocardial tissue of patients undergoing heart surgery and in pancreatic tissue of diabetics. EV capsid antigens are expressed in cardiomyocytes of subjects with chronic cardiomyopathy 14 , 46 , 51 , 52 , as well as in pancreatic beta cells 53 – 55 and lymphoid cells of diabetic patients 29 , 56 . Notably, Norwegian investigators succeeded in detecting EV infection in pancreata of type 1 diabetics at the clinical onset 9 , 57 .…”

Section: Discussionmentioning

confidence: 99%

“…Persistent EV infections have been studied in cultured cells of monkey, mouse, and human origin [e.g., the HEp2 line, neural and endothelial cells, astrocytes, neuroblastoma lines 27 ]. Cultured cells undergoing persistent infection display peculiar features 27 , 28 : a) cytopathic effect (CPE) is not evident, b) viral antigens are expressed in a small percentage of cells, c) virus is produced at low titers (≤10 3 plaque-forming units/ml), d) cytokines and chemokines are secreted 29 . In persistently infected cultures, virus transmission may occur through the release of extracellular vesicles, formation of cell protrusions and intercellular bridges 8 , 25 , 26 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Revealing enterovirus infection in chronic human disorders: An integrated diagnostic approach

Genoni

Canducci

Rossi

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Enteroviruses (EVs) causing persisting infection are characterized by minimal replication and genetic changes. Typing of these agents may complement disease assessment and shed light on pathogenesis. Here we report an integrated approach for EV detection in human samples that is based on pre-enrichment of virus in cell culture before search for the viral genome and viral antigens. Cases of post-polio syndrome, type 1 diabetes, and chronic cardiomyopathy were investigated. As tissue-based approaches require invasive procedures, information was mainly gleaned from virus in blood. Molecular assays targeting conserved genome regions of all EV types (5′UTR, 2 C, 3Dpol) were employed. As compared to direct assays of plasma or leukocytes, the EV detection rate was significantly enhanced by co-culture of leukocytes with cell lines prior to molecular and immunologic tests. Results of RT-PCR and sequencing were confirmed by staining cell cultures with a panel of EV-specific antibodies. Sequence and phylogenetic analysis showed that EVs of the C species (polioviruses) were associated with the post-polio syndrome, while members of the B species were found in type 1 diabetes and cardiomyopathy. The procedure may be used for investigating the possible association of different EVs with a variety of chronic neurologic, endocrine, and cardiac disorders.

show abstract