Distension of the uterus induces HspB1 expression in rat uterine smooth muscle

White, Bryan G.; MacPhee, Daniel J.

doi:10.1152/ajpregu.00272.2011

Cited by 7 publications

(9 citation statements)

References 53 publications

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“…Our Pathway Studio results revealed that HSPB1 is significantly associated with reproductive disease (implantation failure). It is reported that HSPB1 protein is overexpressed in uterine distension during pregnancy, as well as inducing the distension of the myometrium, owing to the growing fetus [ 123 , 124 ].…”

Section: Resultsmentioning

confidence: 99%

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Ryu

Rahman

Pang

2017

IJMS

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Bisphenol-A (BPA) is a ubiquitous endocrine-disrupting chemical. Recently, many issues have arisen surrounding the disease pathogenesis of BPA. Therefore, several studies have been conducted to investigate the proteomic biomarkers of BPA that are associated with disease processes. However, studies on identifying highly sensitive biological cell model systems in determining BPA health risk are lacking. Here, we determined suitable cell model systems and potential biomarkers for predicting BPA-mediated disease using the bioinformatics tool Pathway Studio. We compiled known BPA-mediated diseases in humans, which were categorized into five major types. Subsequently, we investigated the differentially expressed proteins following BPA exposure in several cell types, and analyzed the efficacy of altered proteins to investigate their associations with BPA-mediated diseases. Our results demonstrated that colon cancer cells (SW480), mammary gland, and Sertoli cells were highly sensitive biological model systems, because of the efficacy of predicting the majority of BPA-mediated diseases. We selected glucose-6-phosphate dehydrogenase (G6PD), cytochrome b-c1 complex subunit 1 (UQCRC1), and voltage-dependent anion-selective channel protein 2 (VDAC2) as highly sensitive biomarkers to predict BPA-mediated diseases. Furthermore, we summarized proteomic studies in spermatozoa following BPA exposure, which have recently been considered as another suitable cell type for predicting BPA-mediated diseases.

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Section: Resultsmentioning

confidence: 99%

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Ryu

Rahman

Pang

2017

IJMS

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“…This model has been previously used to study the effect of uterine distension or stretch on numerous genes and proteins including the gap junction protein GJA1 (Ou et al 1997), the heat shock protein CRYAB (Nicoletti et al 2016) and signaling kinases such as MAPK14 (Oldenhof et al 2002). Virgin female rats were anaesthetized and underwent a unilateral tubal ligation procedure as previously described (White and MacPhee 2011). Animals were monitored post-operatively and allowed to recover for at least 5 days before matings were attempted.…”

Section: Tissue Collectionmentioning

confidence: 99%

“…We previously reported that Hspb1 mRNA expression was significantly increased during late pregnancy in the rat myometrium, that pS15-HSPB1 detection significantly increased during late pregnancy and labour, and that pS15-HSPB1 was induced by uterine distension (White et al. 2005 ; White and MacPhee 2011 ). Furthermore, pS15-HSPB1 had a predominantly membrane-associated localization in situ within cells of the circular and longitudinal muscle layers; however, pS86-HSPB1 was not examined.…”

Section: Introductionmentioning

confidence: 99%

Phosphoserine-86-HSPB1 (pS86-HSPB1) is cytoplasmic and highly induced in rat myometrium at labour

Miśkiewicz

Olaloku

MacPhee

et al. 2022

Histochem Cell Biol

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Uterine myocytes during pregnancy proceed through a series of adaptations and collectively transform into a powerfully contractile tissue by term. Previous work has indicated that members of the heat shock protein (HSP) B family of stress proteins are associated with the process of adaptation and transformation. Utilizing immunoblot analyses, widefield epifluorescence and total internal reflection (TIRF) microscopy, this study investigated the temporal and spatial detection of HSPB1 phosphorylated on serine-86 (pS86-HSPB1) in rat myometrium during pregnancy, the role of uterine distension in regulation of pS86-HSPB1, and the comparative localization with pS15-HSPB1 in rat myometrial tissue as well as in an immortalized human myometrial cell line. Immunoblot detection of pS86-HSPB1 was significantly elevated during late pregnancy and labour. In particular, pS86-HSPB1 was significantly increased at day (d)22 and d23 (labour) compared with all other timepoints assessed. Localization of pS86-HSPB1 in myometrium became prominent at d22 and d23 with cytoplasmic detection around myometrial cell nuclei. Furthermore, pS86-HSPB1 detection was found to be significantly elevated in the gravid rat uterine myometrium compared with the non-gravid tissue at d19 and d23. Both widefield epifluorescence and TIRF microscopy examination of human myometrial cells demonstrated that pS15-HSPB1 was prominently localized to focal adhesions, while pS82-HSPB1 (homologous to rodent pS86-HSPB1) was primarily located in the cell cytoplasm. Our data demonstrate that levels of phosphorylated HSPB1 increase just prior to and during labour, and that uterine distension is a stress-inducing signal for HSPB1 phosphorylation. The exact roles of these phosphorylated forms in myometrial cells remain to be determined.

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“…This model has been used previously to study the effect of uterine distension or stretch on numerous genes and proteins, including the gap junction protein GJA1, the heat shock protein aBcrystallin (CRYAB) and signalling kinases such as mitogenactivated protein kinase (MAPK) 14 (Ou et al 1997;Oldenhof et al 2002;Nicoletti et al 2016). Virgin female rats were anaesthetised and underwent a unilateral tubal ligation procedure as described previously in detail by White and MacPhee (2011). Animals were monitored postoperatively and allowed to recover for at least 5 days before matings were attempted.…”

Section: Tissue Collectionmentioning

confidence: 99%

Inducible heat shock protein A1A (HSPA1A) is markedly expressed in rat myometrium by labour and secreted via myometrial cell-derived extracellular vesicles

Russell

Bailey

Miśkiewicz

et al. 2021

Reprod. Fertil. Dev.

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The myometrium goes through physiological, cellular and molecular alterations during gestation that necessitate effective cellular proteostasis. Inducible heat shock protein A1A (HSPA1A) is a member of the 70-kDa heat shock protein A (HSPA) family, which acts as a chaperone to regulate proteostasis; however, HSPA1A also participates as a cytokine in inflammatory regulation, leading to its designation as a chaperokine. This study examined the spatiotemporal expression of HSPA1A protein in the rat myometrium throughout gestation and assessed whether it is secreted as cargo of myometrial cell-derived extracellular vesicles (EVs). Immunoblot analysis demonstrated that HSPA1A expression was markedly elevated during late pregnancy and labour and increased by uterine distension. Myometrial HSPA1A expression insitu increased in myocytes of longitudinal and circular muscle layers from Day 19 through to postpartum, specifically in the cytoplasm and nuclei of myocytes from both muscle layers, but frequently detectable just outside myocyte membranes. Scanning electron microscopy examination of samples isolated from hTERT-HM cell-conditioned culture medium, using EV isolation spin columns, confirmed the presence of EVs. EV lysates contained HSPA8, HSPA1A and the EV markers apoptosis-linked gene 2-interacting protein X (Alix), the tetraspanin cluster of differentiation 63 (CD63), tumour susceptibility gene 101 (TSG101) and HSP90, but not the endoplasmic reticulum protein calnexin. These results indicate that HSPA1A may act as a chaperokine in the myometrium during pregnancy.

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Distension of the uterus induces HspB1 expression in rat uterine smooth muscle

Abstract: White BG, MacPhee DJ. Distension of the uterus induces HspB1 expression in rat uterine smooth muscle.

Cited by 7 publications

References 53 publications

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Determination of Highly Sensitive Biological Cell Model Systems to Screen BPA-Related Health Hazards Using Pathway Studio

Phosphoserine-86-HSPB1 (pS86-HSPB1) is cytoplasmic and highly induced in rat myometrium at labour

Inducible heat shock protein A1A (HSPA1A) is markedly expressed in rat myometrium by labour and secreted via myometrial cell-derived extracellular vesicles

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