Distance-Dependence Study of Plasmon Resonance Energy Transfer with DNA Spacers

Abstract

Plasmon resonance energy transfer (PRET) in hybrid plasmonic−molecular systems has a broad range of applications from catalysis to analytical/biochemical/biophysical imaging and sensing. Herein, we experimentally and theoretically probed the influence of the distance (d) between the plasmonic nanoparticle and the conjugated molecules on the PRET efficiency (η PRET ) using two PRET systems, which involved tetramethylrhodamine (TAMRA) or Cy3 molecules as acceptors and single spherical gold nanoparticles (AuNPs) as donors. The double-stranded DNA (dsDNA) sequences precisely adjusted within 12.0 nm were utilized as a donor−acceptor spacer. Then, the η PRET of the two systems under varied d-values was available from the reduction of the scattering intensity of AuNPs. Both experimental and quasi-static approximation data show that η PRET displays a d-value-dependent decay function. This study would provide new insights into optimal PRET-based chemical/biochemical sensors.

Dark-Field Microscopy: Recent Advances in Accurate Analysis and Emerging Applications

“…Both the experimental and quasi-static approximation data showed that η PRET displayed a d -value-dependent decay function. This research provided systematic insights into the widely used PRET technique …”

“…This research provided systematic insights into the widely used PRET technique. 54 Plasmon Ruler. Another powerful tool in the iDFM is the plasmon ruler, which is based on the plasmon resonance coupling effect.…”

Plasmonic gold nanostructures for biosensing and bioimaging

“…In this way, the “loading-type” plasmonic nanosensor could realize the ONOO − determination with good selectivity and sensitivity (at a single nanoparticle level). Since PRET has broad applications in biosensing, the effect of the distance (d) between the conjugated molecules and the plasmonic nanoparticle on the PRET efficiency (ηPRET) has been investigated by utilizing Cy3 molecules or tetramethylrhodamine as acceptors and single spherical AuNPs as donors [ 53 ]. Double-stranded DNA was applied to regulate the d -values between the donor and the acceptor.…”

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“…[ 188 ] Furthermore, Ma and coworkers experimentally and theoretically probed the influence of the distance ( d ) between the PNP and the conjugated molecules on the PRET efficiency, and found that the PRET efficiency displayed a d ‐value‐dependent decay function. [ 189 ] Therefore, the quenching efficiency of PRET can be regulated by changing the distance between PNPs donor and molecules acceptor, indicating the applicability of the turn‐on and turn‐off types of sensing mechanisms. Liu and coworkers utilized quantum dots (QDs)‐AuNPs pair, in which PRET occurred from AuNPs to QD 800, to develop separation‐free homogeneous immunoassays for quantifying PSA (Figure 6C).…”