Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami

The control over the defined assembly of nano-objects with nm-precision is important to create systems and materials with enhanced properties, for example, metamaterials. In nature, the precise assembly of inorganic nano-objects with unique features, for example, magnetosomes, is accomplished by efficient and reliable recognition schemes involving protein effectors. Here we present a molecular approach using protein-based 'adaptors/ connectors' with genetically encoded interaction sites to guide the assembly and functionality of different plasmonically active gold nanoparticle architectures (AuNP). The interaction of the defined geometricaly shaped protein adaptors with the AuNP induces the self-assembly of nanoarchitectures ranging from AuNP encapsulation to one-dimensional chain-like structures, complex networks and stars. Synthetic biology and bionanotechnology are applied to co-translationally encode unnatural amino acids as additional site-specific modification sites to generate functionalized biohybrid nanoarchitectures. This protein adaptor-based nanoobject assembly approach might be expanded to other inorganic nano-objects creating biohybrid materials with unique electronic, photonic, plasmonic and magnetic properties.

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“…51) up to 99% at 1 nm (ref. 50) distances between AuNP and dye for AuNPs of similar size (10 and 30 nm, respectively).…”

Section: Resultsmentioning

confidence: 99%

Molecular protein adaptor with genetically encoded interaction sites guiding the hierarchical assembly of plasmonically active nanoparticle architectures

et al. 2015

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“…Clearly, some factors such as size, spacer distance, dielectric environment, and the shape of the metal nanostructures significantly influence the plasmon resonance frequency, and thus play important roles in plasmon‐enhanced upconversion emission. [[qv: 65c]],67 Stucky et al fabricated Ag@SiO 2 @Y 2 O 3 :Er nanostructures. [[qv: 65c]] After coating Ag NPs with a silica shell, a red‐shift is typically observed due to the local refractive index change around the particles.…”

Section: Discussionmentioning

confidence: 99%

Plasmon‐Mediated Solar Energy Conversion via Photocatalysis in Noble Metal/Semiconductor Composites

et al. 2016

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Plasmonics has remained a prominent and growing field over the past several decades. The coupling of various chemical and photo phenomenon has sparked considerable interest in plasmon‐mediated photocatalysis. Given plasmonic photocatalysis has only been developed for a relatively short period, considerable progress has been made in improving the absorption across the full solar spectrum and the efficiency of photo‐generated charge carrier separation. With recent advances in fundamental (i.e., mechanisms) and experimental studies (i.e., the influence of size, geometry, surrounding dielectric field, etc.) on plasmon‐mediated photocatalysis, the rational design and synthesis of metal/semiconductor hybrid nanostructure photocatalysts has been realized. This review seeks to highlight the recent impressive developments in plasmon‐mediated photocatalytic mechanisms (i.e., Schottky junction, direct electron transfer, enhanced local electric field, plasmon resonant energy transfer, and scattering and heating effects), summarize a set of factors (i.e., size, geometry, dielectric environment, loading amount and composition of plasmonic metal, and nanostructure and properties of semiconductors) that largely affect plasmonic photocatalysis, and finally conclude with a perspective on future directions within this rich field of research.

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“…The presence of two lifetime populations at ≈240 and ≈370 ps for control BSA hydrogel is likely due to self‐quenching of RB molecules 54. The reduction in lifetime may be attributed to the resonance energy transfer from the fluorophore to the AuNT, confirming the immobilization of BSA hydrogel on the AuNT 53, 55…”

Section: Resultsmentioning

confidence: 67%

Regioselective Localization and Tracking of Biomolecules on Single Gold Nanoparticles

et al. 2015

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Selective localization of biomolecules at the hot spots of a plasmonic nanoparticle is an attractive strategy to exploit the light–matter interaction due to the high field concentration. Current approaches for hot spot targeting are time‐consuming and involve prior knowledge of the hot spots. Multiphoton plasmonic lithography is employed to rapidly immobilize bovine serum albumin (BSA) hydrogel at the hot spot tips of a single gold nanotriangle (AuNT). Regioselectivity and quantity control by manipulating the polarization and intensity of the incident laser are also established. Single AuNTs are tracked using dark‐field scattering spectroscopy and scanning electron microscopy to characterize the regioselective process. Fluorescence lifetime measurements further confirm BSA immobilization on the AuNTs. Here, the AuNT‐BSA hydrogel complexes, in conjunction with single‐particle optical monitoring, can act as a framework for understanding light–molecule interactions at the subnanoparticle level and has potential applications in biophotonics, nanomedicine, and life sciences.

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Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami

Cited by 284 publications

References 41 publications

Molecular protein adaptor with genetically encoded interaction sites guiding the hierarchical assembly of plasmonically active nanoparticle architectures

Molecular protein adaptor with genetically encoded interaction sites guiding the hierarchical assembly of plasmonically active nanoparticle architectures

Plasmon‐Mediated Solar Energy Conversion via Photocatalysis in Noble Metal/Semiconductor Composites

Regioselective Localization and Tracking of Biomolecules on Single Gold Nanoparticles

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