Distance-based β-amyloid protein detection on PADs for the scanning and subsequent follow-up of Alzheimer's disease in human urine samples

Abstract

We report on the first development of a simple distance-based β-amyloid (Aβ) protein quantification using paper-based devices (dPADs) to screen for Alzheimer’s disease (AD) and to subsequently follow up on...

A Simple Counting-Based Measurement for Paper Analytical Devices and Their Application

“…We further examined the relative uncertainty of using the naked-eye detection method by measuring the signal of this level of CBR for 10 times reproducibility (n = 10 (Figure S9). 32,33 Accordingly, the average counting signals of this CBR concentration were at 1.0 lines with a standard deviation (SD) at 0.33. This allowed us to obtain the uncertainty measurement at a 99% confidence interval starting at 1.0 ± 0.32 lines, and this is within the range of the 0.68−1.32 lines for the counting signals.…”

Distance-based paper analytical device for multiplexed quantification of cytokine biomarkers using carbon dots integrated with molecularly imprinted polymer

“…The influence of the sample volume is significant to microfluidic analysis since it is associated with the amount of the target analyte presents in the solution. [20][21][22][23] We studied sample volumes between 30.0 and 90.0 μL containing CRP (12.0 pg mL −1 ), TNF-α (2.0 pg mL −1 ), and IL-6 (10.0 pg mL −1 ), then monitored the quenched fluorescence distance signals as shown in Fig. 2(a).…”

“…The extent of the distance length provides a quantitative detection proportional to the analyte concentration. [20][21][22] With these characteristics, this measurement format is simpler than colorimetric, fluorescence, and electrochemical techniques, and also can be operated by untrained individuals. Furthermore, this technique can reduce errors that are common in colorimetric and fluorescence measurements from ambient lighting or background autofluorescence.…”

Quantification of clofarabine in urine and plasma by LC-MS/MS: suitable for PK study and TDM in pediatric patients with relapsed or refractory ALL

“…Current practice includes monitoring clofarabine elimination via serum concentration, a lot of approaches have been reported for the determination of clofarabine concentration in blood. 1,[13][14][15][16] However, considering that the blood collection is an invasive operation, this process could easily cause infection in the patient, 17 and the volume of blood collected is usually limited in pediatric patients with ALL. 18 The clinical application of these studies is limited.…”