Distal oxidative metabolic responses induced by epidermal injury

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Twelve young BNL mice received a “gingivectomy” excising the gingival papilla mesial to the left maxillary first molar. Twelve additional young mice served as uninjured controls. One hour prior to sacrifice, each animal received a subcutaneous injection of 1 μCi H3‐TDR per gm body weight. Animals were sacrificed 2, 4, 6, and 8 days after injury. At time of sacrifice, maxillae, femora, and a portion of abdominal skin were obtained from each animal for histologic and autoradiographic analysis. In most tissues analyzed, the gingivectomy led to a significant reduction in labeling indices at tissue sites distant from the gingivectomy. This effect was short‐lived, however, and could not be noted at time periods later than two days post‐injury. Since such distal effects are of short duration, it is questionable whether they would affect therapeutic sequences currently used in periodontal treatment.

Section: Discussionsupporting

confidence: 92%

Section: Introductionsupporting

confidence: 54%

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Autoradiographic assessment of the distal response to gingival injury

Tonna

Stahl

Slywka

1977

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“…Tbe mirror effect of matrix formation observed at tbe control site in relation to tbe site of injury, is also of interest. Recently we described botb biocbemical and ce:llular responses at sites distal to tbe injury itself (Fine et al 1976, Tonna et al 1977, and one wonders wbetber a distal effect to injury may also be expressed in tbe rates of matrix formation.…”

Section: Clinical Significancementioning

confidence: 98%

A study of the reformation of severed gingival fibers in aging mice using ³H‐proline autoradiography

Tonna

Stahl

Asiedu

1980

The present study monitored the healing responses at sites of attached but severed gingival fibers by following matrix production and turnover using 3H‐proline in mice of different ages. Fifty‐six BNL short‐lived mice of mixed sex to 78‐week‐old were used. A vertical gingival incision was made at the mesial papilla of the right maxillary first molar to the alveolar bone crest severing the gingival fibers. The left side was used as a control. Periodontal regions adjacent to the incision and some distance from the incision were studied. All mice were given a s.q. dose of 2μCi of 3H‐proline/gm. b. wt. and killed from 1 to 90 days later. Autoradiographs were prepared from routinely processed histologic sections for grain counting. The control crestal region exhibited the greatest matrical turnover of all sites tested regardless of age. With increasing age, however, the rates of uptake and turnover were significantly reduced in all regions. Post‐injury, additional peaks of activity occurred in young mice. In old mice, the injured cemental side exhibited significant uptake and prolonged matrical turnover. It was concluded that the matrix on the injured corium side was the most stable, while the cemental region was the least stable. Aging did not impede the tissue response to injury but increased the long term response.

“…The purpose of this study was to evaluate the effects of glucocorticoids on gitigival oxidative metabolism at sites of gingival injury, sites distal to injury and at noninjured control sites. This problem emerged as a result of findings demonstrating that 1, gingival injury activated oxidative metabolism at distal (nan-injured) sites (Fine et al 1976); 2, trauma induced an inhibition of the mitotic index two days after injtury at distal and distant sites (Tonna et al 1977); 3, skin incision led to alterations in collagen solubility at sites distal to injury (Peacock 1963); and 4, cortisol injections indace collagen changes similar to those seen at sites distal to skin injury (Houck 1965).…”

Section: Introductionmentioning

confidence: 99%

Methylprednisolone effects upon cytochrome oxidase activity during gingival wound healing in rats

Fine

Egnor

Scopp

et al. 1977

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Methylprednisolone injected intraperitoneally in rats (pharmacological doses) during, a short time (1-2 days) interval activated gingival cytochrome oxidase activity at drug treated sham, and drug treated wound and distal (contralateral) non-injured sites. Gingival cytochrome oxidase was activated in drug treated sham and injured gingiva between the 5th and 7th days after drug (2 doses) initiation. Enzymatic activities in both groups declined between the 7th and lOth days toward control levels. An increase in enzymatic activity occurred within 1 day at the distal (non-injured) contralateral site in animals which received only one dose of methylprednisolone. The enzymatic activity declined between the 1st and 2nd day to controi vaiues aod remained at that level for the duration of the experiment.