Dissolving microneedle-encapsulated drug-loaded nanoparticles and recombinant humanized collagen type III for the treatment of chronic wound <i>via</i> anti-inflammation and enhanced cell proliferation and angiogenesis

Long, Linyu; Liu, Wenqi; Li, Li; Hu, Cheng; He, Shuyi; Lu, Lu; Wang, Jian; Yang, Li; Wang, Yunbing

doi:10.1039/d1nr07708b

Cited by 45 publications

(23 citation statements)

References 62 publications

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“…It can also deliver effective drugs in order according to the wound conditions. It is worth mentioning that rh col III is synthesized based on Gly483-Pro512 fragment and has strong cell adhesion (Long et al, 2022).…”

Section: Internal Environment Responsivenessmentioning

confidence: 99%

Effect of composite biodegradable biomaterials on wound healing in diabetes

Ren

Guo²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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The repair of diabetic wounds has always been a job that doctors could not tackle quickly in plastic surgery. To solve this problem, it has become an important direction to use biocompatible biodegradable biomaterials as scaffolds or dressing loaded with a variety of active substances or cells, to construct a wound repair system integrating materials, cells, and growth factors. In terms of wound healing, composite biodegradable biomaterials show strong biocompatibility and the ability to promote wound healing. This review describes the multifaceted integration of biomaterials with drugs, stem cells, and active agents. In wounds, stem cells and their secreted exosomes regulate immune responses and inflammation. They promote angiogenesis, accelerate skin cell proliferation and re-epithelialization, and regulate collagen remodeling that inhibits scar hyperplasia. In the process of continuous combination with new materials, a series of materials that can be well matched with active ingredients such as cells or drugs are derived for precise delivery and controlled release of drugs. The ultimate goal of material development is clinical transformation. At present, the types of materials for clinical application are still relatively single, and the bottleneck is that the functions of emerging materials have not yet reached a stable and effective degree. The development of biomaterials that can be further translated into clinical practice will become the focus of research.

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Section: Internal Environment Responsivenessmentioning

confidence: 99%

Effect of composite biodegradable biomaterials on wound healing in diabetes

Ren

Guo²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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“…CD31 is a transmembrane protein expressed in early angiogenesis, indicating neovascularization, while α-SMA is a cytoplasmic protein expressed in later angiogenesis, indicating the maturation of vascular smooth muscle cells [ 34 ]. The vascular network plays a key role in the repair of full-thickness skin due to its function of transporting nutrients, oxygen and immune cells into the wound area [ 26 ]. CD31/α-SMA immunofluorescence was performed on the wound tissues at 7 and 14 days.…”

Section: Resultsmentioning

confidence: 99%

“…Recombinant collagen is a novel biomaterial manufactured by synthetic biology techniques and is a candidate material for various medical applications [ 25 ]. Type III collagen is a major component of the extracellular matrix in internal organs and skin in adults [ 26 ]. The recombinant human collagen III protein (rhCol III) is the product of a re-optimized design gene sequence based on the characteristics and main functional domains of human collagen.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing

Liu

Tang

et al. 2022

IJMS

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Existing treatment methods encounter difficulties in effectively promoting skin wound healing, making this a serious challenge for clinical treatment. Extracellular vesicles (EVs) secreted by stem cells have been proven to contribute to the regeneration and repair of wound tissue, but they cannot be targeted and sustained, which seriously limits their current therapeutic potential. The recombinant human collagen III protein (rhCol III) has the advantages of good water solubility, an absence of hidden viral dangers, a low rejection rate and a stable production process. In order to achieve a site-specific sustained release of EVs, we prepared a rhCol III hydrogel by cross-linking with transglutaminase (TGase) from Streptomyces mobaraensis, which has a uniform pore size and good biocompatibility. The release profile of the rhCol III-EVs hydrogel confirmed that the rhCol III hydrogel could slowly release EVs into the external environment. Herein, the rhCol III-EVs hydrogel effectively promoted macrophage changing from type M1 to type M2, the migration ability of L929 cells and the angiogenesis of human umbilical vein endothelial cells (HUVECs). Furthermore, the rhCol III-EVs hydrogel is shown to promote wound healing by inhibiting the inflammatory response and promoting cell proliferation and angiogenesis in a diabetic rat skin injury model. The reported results indicate that the rhCol III-EVs hydrogel could be used as a new biological material for EV delivery, and has a significant application value in skin wound healing.

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Section: Mnsmentioning

confidence: 99%

“…67 To suppress persistent inflammation and ameliorate adverse tissue remodeling, Wang et al prepared a HA MN loaded with recombinant humanized collagen type III (rhCol III) and naproxen (Nap) for the treatment of diabetic chronic wounds. 68 To enable the sustained release of the drug to the wound site for a long time, they encapsulated Nap in poly(lactic- co -glycolic acid) nanoparticles, thereby reducing frequent dressing changes, avoiding the sudden release of the drug and alleviating inflammatory response. Results of the animal experiment suggested that the prepared MNs could accelerate diabetic chronic wound healing by reducing the inflammatory response and enhancing angiogenesis and collagen deposition.…”

Section: Categories Of Multifunctional Wound Dressingsmentioning

confidence: 99%

Construction of multifunctional wound dressings with their application in chronic wound treatment

Long

Liu

et al. 2022

Biomater. Sci.

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Dissolving microneedle-encapsulated drug-loaded nanoparticles and recombinant humanized collagen type III for the treatment of chronic wound via anti-inflammation and enhanced cell proliferation and angiogenesis

Abstract: The first recombinant humanized collagen type III (rhCol III) and naproxen (Nap) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles incorporated hyaluronic acid (HA) microneedle (MN) was fabricated for diabetic chronic wounds therapy.

Cited by 45 publications

References 62 publications

Effect of composite biodegradable biomaterials on wound healing in diabetes

Effect of composite biodegradable biomaterials on wound healing in diabetes

Preparation of Recombinant Human Collagen III Protein Hydrogels with Sustained Release of Extracellular Vesicles for Skin Wound Healing

Construction of multifunctional wound dressings with their application in chronic wound treatment

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