Dissolved ATP in the sea and its utilisation by marine bacteria

Azam, Farooq; Hodson, Robert E.

doi:10.1038/267696a0

Cited by 134 publications

(96 citation statements)

References 6 publications

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“…For instance, the compound ATP, which is analyzed here, represents a model substrate of the organic-P pool (Azam and Hodson 1977). Yet, we found that major taxonomic bacterial groups significantly differed in the mean values and ranges of percent active cells using glucose, amino acids, or ATP (Fig.…”

Section: Discussionmentioning

confidence: 99%

Bacterial uptake of low molecular weight organics in the subtropical Atlantic: Are major phylogenetic groups functionally different?

Alonso‐Sáez

Sánchez

Gasol

2012

Limnology & Oceanography

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We present measurements of glucose, amino acids, and adenosine triphosphate (ATP) bacterial uptake at tracer concentrations across an oceanic gradient from the Cape Blanc upwelling to the Northeast Atlantic subtropical gyre. The bulk uptake of the compounds increased in the upwelling, with amino acids being the most actively taken up substrate (up to 20 pmol L 21 h 21 ). The single-cell activity of the bacterial groups also increased in the upwelling, particularly for Rhodobacteraceae (up to 94% of active cells), but this group had low activity in oligotrophic waters (, 10% of active cells), which suggests it is exclusively adapted to high-nutrient conditions. The percentage of SAR11 active cells was relatively high in the upwelling area, particularly for glucose and amino acid uptake (up to 53% of active cells), which suggests that some members of this group are also adapted to nutrient-rich environments. Of the broad phylogenetic groups tested, Bacteroidetes were the least active and Alpha-and Gammaproteobacteria showed similar percentages of active cells in amino acid uptake (, 30%). Alphaproteobacteria had the highest percent of cells involved in glucose uptake, while Gammaproteobacteria dominated ATP uptake. This general pattern was confirmed in a broader analysis that included data from contrasting marine environments, which suggests that major phylogenetic groups of bacteria participate differently in the turnover of these low-molecular-weight organics. Our results support the view that broad phylogenetic groups can be identified within the bacterial 'black box' with different patterns in the cycling of organic matter. Analyzing them may help us understand, and ultimately predict, oceanic carbon processing.

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Section: Discussionmentioning

confidence: 99%

Bacterial uptake of low molecular weight organics in the subtropical Atlantic: Are major phylogenetic groups functionally different?

Alonso‐Sáez

Sánchez

Gasol

2012

Limnology & Oceanography

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“…[60] For example in the North Pacific subtropical Gyre, because ATP is both rapidly produced and rapidly consumed, it was estimated that the P flux through the ATP pool was up to five times faster than the general organic P pool. [55] Similarly in a mesocosm experiment Løvdal et al [49] showed that the turnover time of ATP under P-limited conditions was only 5 min.…”

Section: Ecological Significancementioning

confidence: 99%

Organic phosphorus in the aquatic environment

Baldwin¹

2013

Environ. Chem.

104

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Environmental context. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. This paper discusses the distribution, cycling and ecological significance of five major classes of organic P in the aquatic environment and discusses several principles to guide organic P research into the future.Abstract. Organic phosphorus can be one of the major fractions of phosphorus in many aquatic ecosystems. Unfortunately, in many studies the 'organic' P fraction is operationally defined. However, there are an increasing number of studies where the organic P species have been structurally characterised -in part because of the adoption of 31 P NMR spectroscopic techniques. There are five classes of organic P species that have been specifically identified in the aquatic environment -nucleic acids, other nucleotides, inositol phosphates, phospholipids and phosphonates. This paper explores the identification, quantification, biogeochemical cycling and ecological significance of these organic P compounds. Based on this analysis, the paper then identifies a number of principles which could guide the research of organic P into the future. There is an ongoing need to develop methods for quickly and accurately identifying and quantifying organic P species in the environment. The types of ecosystems in which organic P dynamics are studied needs to be expanded; flowing waters, floodplains and small wetlands are currently all under-represented in the literature. While enzymatic hydrolysis is an important transformation pathway for the breakdown of organic P, more effort needs to be directed towards studying other potential transformation pathways. Similarly effort should be directed to estimating the rates of transformations, not simply reporting on the concentrations. And finally, further work is needed in elucidating other roles of organic P in the environment other than simply a source of P to aquatic organisms.

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“…Dissolved nucleic acids, nucleotides and nucleotide derivatives are ubiquitous components of the dissolved organic matter (DOM) pool in aquatic environments (Minear 1972, Azam & Hodson 1977, Karl & Bailiff 1989. Dissolved adenosine-5'-triphosphate (D-ATP) has been reported to occur in a wide variety of aquatic habitats ranging from eutrophic, freshwater lakes to temperate, marine coastal regions and Antarctic seawaters (Azam & Hodson 1977, Riemann 1979, Hodson et al 1981a, McGrath & Sullivan 1981, Maki et al 1983, Nawrocki & Karl 1989.…”

Section: Introductionmentioning

confidence: 99%

Presence of dissolved nucleotides in the North Pacific Subtropical Gyre and their role in cycling of dissolved organic phosphorus

Björkman¹,

Karl²

2005

Aquat. Microb. Ecol.

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Dissolved and particulate nucleotide triphosphate (NTP) concentrations were measured in the upper 1000 m of the water column during 3 summer and 3 winter months at Stn ALOHA (22.75°N, 158°W) in the oligotrophic North Pacific Subtropical Gyre. In the euphotic zone (0 to 175 m) particulate adenosine-5'-triphosphate (P-ATP) and dissolved ATP (D-ATP) concentrations were positively correlated in summer (0 to 175 m; r 2 = 0.61, p < 0.001, n = 24), but not in winter (r 2 = 0.02, n = 24). D-ATP comprised > 65% of the total ATP (T-ATP) pool in the summer, and ~50% in winter. Dissolved guanosine-5'-triphosphate (D-GTP) inventories were 5-to 6-fold greater in July and August than those observed in mid-June (1.1 ± 0.1 vs. 8.0 ± 0.6 and 6.5 ± 0.3 µmol D-GTP m -2 , respectively) and winter concentrations were on average lower than the mean summer concentrations. The particulate GTP (P-GTP) inventories were almost twice those measured in winter (mean 1.1 ± 0.4 in summer vs. 0.6 ± 0.1 µmol P-GTP m -2 in winter, n = 3). These results are consistent with higher microbial growth rates in summer. Uptake of D-ATP showed multi-phasic kinetic patterns. The halfsaturation constants (K t ) ranged from 1 to 26 nM at D-ATP concentrations of 0.2 to 30 nM, and V max ranged from 0.3 to 1.4 nM d , and the turnover time of the ambient D-ATP pool was estimated to be 1 to 2 d. The P flux through the D-ATP pool could potentially be 5 times faster than that of the bulk DOP pool, implying that P derived from nucleotides may be an important pathway in the P cycle of oligotrophic oceans.

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Dissolved ATP in the sea and its utilisation by marine bacteria

Cited by 134 publications

References 6 publications

Bacterial uptake of low molecular weight organics in the subtropical Atlantic: Are major phylogenetic groups functionally different?

Bacterial uptake of low molecular weight organics in the subtropical Atlantic: Are major phylogenetic groups functionally different?

Organic phosphorus in the aquatic environment

Presence of dissolved nucleotides in the North Pacific Subtropical Gyre and their role in cycling of dissolved organic phosphorus

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