Dissolution kinetics of mesoporous silica nanoparticles in different simulated body fluids

Braun, Katharina; Pochert, Alexander; Beck, Michaela; Fiedler, R; Gruber, Jens; Lindén, Mika

doi:10.1007/s10971-016-4053-9

Cited by 99 publications

(81 citation statements)

References 25 publications

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“…First, they are stable in biological media and should therefore not degrade before reaching the lymph nodes. 29 Second, the synthesis of SiNPs allows for fine tuning of size with narrow size distribution for optimal lymph node targeting and accumulation. Third, it has been shown that SiNPs themselves have the intrinsic ability to act as an adjuvant.…”

Section: Discussionmentioning

confidence: 99%

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Thalhauser

Peterhoff

Wagner

et al. 2020

Journal of Pharmaceutical Sciences

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Inducing immune responses protecting from HIV infection or at least controlling replication poses a huge challenge to modern vaccinology. An increasingly discussed strategy to elicit a potent and broad neutralizing antibody response is the immobilization of HIV's trimeric envelope (Env) surface receptor on a nanoparticulate carrier. As a conceptual proof, we attached an Env variant (BG505 SOSIP.664) to highly stable and biocompatible silica nanoparticles (SiNPs) via site-specific covalent conjugation or nonspecific adsorption to SiNPs. First, we demonstrated the feasibility of SiNPs as platform for Env presentation by a thorough characterization process during which Env density, attachment stability, and antigenicity were evaluated for both formulations. Binding affinities to selected antibodies were in the low nanomolar range for both formulations confirming that the structural integrity of Env is retained after attachment. Second, we explored the recognition of SiNP conjugates by antigen presenting cells. Here, the uptake of Env attached to SiNPs via a site-specific covalent conjugation was 4.5-fold enhanced, whereas adsorbed Env resulted only in a moderate 1.4-fold increase compared with Env in its soluble form. Thus, we propose SiNPs with site-specifically and covalently conjugated Env preferably in a high density as a promising candidate for further investigations as vaccine platform.

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Section: Discussionmentioning

confidence: 99%

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Thalhauser

Peterhoff

Wagner

et al. 2020

Journal of Pharmaceutical Sciences

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“…Calcined MSNs were found to be more resistant to degradation in physiological media compared with template‐extracted ones. Lindén and co‐workers showed that specific surface area was the main parameter of controlling silica dissolution rates of different MSNs studied . Quignard et al reported a significant decrease in the size of fluorescent nonporous fluorescein isothiocyanate (FITC)–SiO 2 NPs located in endocytic vesicles after 14 days, on the basis of TEM observation, and the dissolution process was proved by both the detected colloidal and soluble silica species in the cell culture medium .…”

Section: Introductionmentioning

confidence: 98%

“…However, silica can slowly degrade in aqueous media because SiOSi bonds slowly hydrolyze into two SiOH units, and their degradation behaviors appear to be complex, depending on many factors, such as framework condensation degree (i.e., SiOSi and SiOH contents), particle size and concentration, specific surface area, pore size and texture, aggregation degree among MSNs, functionalization groups, and the presence of organic/inorganic species into the silica framework, solution type, temperature, etc. ; He et al reported a three‐stage degradation behavior of surfactant‐extracted MCM‐41‐type MSNs in simulated body fluid (SBF), including 1) a fast degradation stage on an hour scale 1), 2) then a decelerated degradation stage due to the formation of calcium/magnesium silicate layer on the MSNs surface 2), and 3) finally, a maintained slow diffusion stage on a dayscale 3), with a nearly complete degradation after a 15 day immersion at the particle concentration of 0.5 mg mL −1 . Calcined MSNs were found to be more resistant to degradation in physiological media compared with template‐extracted ones.…”

Section: Introductionmentioning

confidence: 99%

Disulfide‐Bridged Organosilica Frameworks: Designed, Synthesis, Redox‐Triggered Biodegradation, and Nanobiomedical Applications

Kleitz

et al. 2018

Adv Funct Materials

159

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Over the past few years, silica‐based nanotheranostics have demonstrated their great potential for nano/biomedical applications. However, the uncontrollable and difficult degradability of their pure silica framework and long‐time in vivo retention still cause severe and unpredictable toxicity risks. Therefore, it is highly desirable to design and synthesize materials with safer framework structures and compositions. To this aim, the introduction of disulfide bonds into the silica framework can not only maintain high stability in physiological conditions, but also achieve a stimuli‐responsive biodegradation triggered by intracellular reducing microenvironment in living cells, especially in cancer cells. Once nanotheranostics with disulfide (i.e., thioether)‐bridged silsesquioxane framework are taken up by tumor cells via passive or active targeting, the disulfide bonds in the hybrid silica matrix can be cleaved by a high concentration of intracellular glutathione, enabling redox‐triggered biodegradation of the nanosystems for both concomitant release of the loaded therapeutic cargo and in vivo clearance. It is envisioned that such hybrid materials comprised of disulfide‐bridged silsesquioxane frameworks can become promising responsive and biodegradable nanotheranostics. This review summarizes the recent advances in the synthesis of hybrid organosilicas with disulfide‐bridged silsesquioxane frameworks, and discuss their redox‐triggered biodegradation behaviors combined with their biocompatibility and nanobiomedical applications.

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“…These defects are leading to a faster loosening of the particles, and hence yield the initial drug release. One can hypothesize that a continued drug release will depend on dissolution of the silica framework in the body uid, 37 which can lead to a continued particle loosening from the substrate or exposure of the drugs through disintegration of the pore walls at the lm surface. This can be compared to the spin-coated particulate system developed by Wiltschka et al that showed a signicantly increased uptake of particles by the cells and particle-free areas were easily observed around the adhered cells.…”

Section: Model Drug Loading and Releasementioning

confidence: 99%

Cell adherence and drug delivery from particle based mesoporous silica films

Björk

Baumann

Hausladen³

et al. 2019

RSC Adv.

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Spatially and temporally controlled drug delivery is important for implant and tissue engineering applications, as the efficacy and bioavailability of the drug can be enhanced, and can also allow for drugging stem cells at different stages of development. Long-term drug delivery over weeks to months is however difficult to achieve, and coating of 3D surfaces or creating patterned surfaces is a challenge using coating techniques like spin-and dip-coating. In this study, mesoporous films consisting of SBA-15 particles grown onto silicon wafers using wet processing were evaluated as a scaffold for drug delivery.Films with various particle sizes (100-900 nm) and hence thicknesses were grown onto trichloro(octadecyl)silane-functionalized silicon wafers using a direct growth method. Precise patterning of the areas for film growth could be obtained by local removal of the OTS functionalization through laser ablation. The films were incubated with the drug model 3,3 0 -dioctadecyloxacarbocyanine perchlorate (DiO), and murine myoblast cells (C2C12 cells) were seeded onto films with different particle sizes. Confocal laser scanning microscopy (CLSM) was used to study the cell growth, and a vinculinmediated adherence of C2C12 cells on all films was verified. The successful loading of DiO into the films was confirmed by UV-vis and CLSM. It was observed that the drugs did not desorb from the particles during 24 hours in cell culture. During adherent growth on the films for 4 h, small amounts of DiO and separate particles were observed inside single cells. After 24 h, a larger number of particles and a strong DiO signal were recorded in the cells, indicating a particle mediated drug uptake. The vast majority of the DiO-loaded particles remained attached to the substrate also after 24 h of incubation, making the films attractive as longer-term reservoirs for drugs on e.g. medical implants. † Electronic supplementary information (ESI) available: Details on substrate preparation, TEM micrographs of the materials, a CLSM micrograph of a lm aer 24 h of cell cultivation, and a CLSM micrograph of C2C12 cells on a lm. See

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Dissolution kinetics of mesoporous silica nanoparticles in different simulated body fluids

Cited by 99 publications

References 25 publications

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Presentation of HIV-1 Envelope Trimers on the Surface of Silica Nanoparticles

Disulfide‐Bridged Organosilica Frameworks: Designed, Synthesis, Redox‐Triggered Biodegradation, and Nanobiomedical Applications

Cell adherence and drug delivery from particle based mesoporous silica films

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