Dissolution-enhanced emission of 1,3,6,8-tetrakis(<i>p</i>-benzoic acid)pyrene for selectively detecting protamine and “on-to-on” heparin detection in water

Li, Hongtao; Zhang, Yuting; Huang, Yan; Cao, Dapeng; Wang, Shitao

doi:10.1039/d1nj03946f

Cited by 2 publications

(2 citation statements)

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“…Meanwhile, some of the developed fluorescent sensors have long incubation times and relatively low sensitivity. [25] Enhancing the binding strength of fluorescent probes to heparin can greatly shorten the incubation time and improve sensitivity. [26] Supramolecular assembly combining fluorescent detection techniques has attracted increasing attention in designing fluorescent probes due to its simplicity, convenience, and good efficiency.…”

Section: Introductionmentioning

confidence: 99%

“…Ratiometric fluorescence sensors rely on the ratio of the intensities of the two emission wavelengths and can properly reduce environmental influences. Meanwhile, some of the developed fluorescent sensors have long incubation times and relatively low sensitivity [25] . Enhancing the binding strength of fluorescent probes to heparin can greatly shorten the incubation time and improve sensitivity [26] …”

Section: Introductionmentioning

confidence: 99%

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Pyrene‐Based Self‐Assembling Peptide for Ratiometric Detection of Heparin

Shao

Song

et al. 2023

ChemBioChem

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Heparin is a commonly used anticoagulant in clinical practice; however, excessive heparin can cause serious adverse reactions. Convenient and accurate detection of heparin levels is thus very important. In this research, a pyrene-based self-assembling fluorescent peptide PyFFRRR was designed for simple, selective, and efficient heparin detection. The guanidine groups in the arginine residues of PyFFRRR bind tightly with heparin, which is highly sulfated, through electrostatic interactions. Charge neutralization facilitated the self-assembly of PyFFRRR, resulting in its spectral response changing from deep blue monomer fluorescence to green excimer fluorescence. PyFFRRR exhibited excellent sensitivity and selectivity for ratiometric detection of heparin. The binding mechanism was investigated by using spectral and simulation tools, and structural observation. Finally, PyFFRRR was employed in human serum samples for ratiometric detection of heparin.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%