Dissociation, Quantification and Culture of Normal Human Merkel Cells Among Epidermal Cell Populations Derived from Glabrous and Hairy Skin Sites

Fradette, Julie; Larouche, Danielle; Fugère, Claudia; Guignard, Rina; Beauparlant, Annie; Couture, Véronique; Caouette‐Laberge, Louise; Roy, Alphonse; Germain, Lucie

doi:10.1007/978-3-662-10358-6_17

Cited by 2 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A mixed culture of epidermal cells enriched with Merkel cells on the 1st and 3rd cultivation passage served as the basis for this study. Cells were prepared from human abdominoplasty skin micro‐biopsies (abdominoplasty) 41 . Ex vivo skin sections (2–5 mm 2 ) were incubated in 50 ml thermolysin for 3 h at 37°C.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of a hyaluronic acid‐based mesotherapeutic injectable on the gene expression of CLOCK and Klotho proteins, and environmentally induced oxidative stress in human skin cells

Prokopov¹,

Дробинцева

Kvetnoy

et al. 2022

J of Cosmetic Dermatology

View full text Add to dashboard Cite

Objective Normal circadian rhythms are essential to the repair mechanisms of oxidative stress implicated in skin aging. Given reports that hyaluronic acid (HA) homeostasis exhibits a different profile in chronological skin aging, as compared to environmental or extrinsic aging, an improved understanding of the way HA interacts with its surroundings, and the impact of HA injectables in replacing lost HA and encouraging rejuvenation, is of key benefit to skin aging treatments. The objectives of these current studies were twofold. Firstly, to demonstrate the in vitro effects of two lightweight hyaluronic‐based injectables on the expression of CLOCK protein in human skin fibroblasts, and their effects on Klotho protein expression as a marker for circadian rhythms in a combined human keratinocyte and Merkel cell model. Secondly, to ascertain whether these findings could be correlated with in vitro effects on various environmental oxidative stress aging markers (blue light, UVA/UVB, Urban Dust, and IR exposures). Methods Oxidative stress studies were aimed to highlight possible protective effects through different challenge conditions in two models, ex vivo human skin explants and in vitro monolayer cultures of normal human dermal fibroblasts (NHDF). The protective effects of the test products were evaluated against an increase of cyclobutene pyrimidine dimers (CPDs) abundance within epidermal section of ex vivo skin explants after UVA/UVB radiation; effects of blue light on gene expression from NHDFs fibroblasts; effects of pollutants (Urban dust, UbD) on gene expression in NHDFs fibroblasts; and an increase of reactive oxygen species (ROS) production by NHDFs fibroblasts after infrared‐A radiation. Gene expression was assayed and analyzed utilizing microfluidic TaqMan qPCR arrays. CLOCK expression was measured in young and senescing NHDFs by immunostaining, and Klotho and melatonin expression by immunostaining in Merkel cell‐enriched normal adult human epidermal cell cultures. Results In an aging culture of mixed keratinocyte and Merkel skin cells, activation of Klotho expression was induced by the application of both HA test products. Moreover, the HA products increase Klotho protein expression in both Merkel cells and keratinocytes. The observed positive effect of the tested products on melatonin receptors 1A and 1B expression in aging Merkel cell culture and keratinocytes is also interesting. HA‐Y (developed for patients 25+ years old) stimulated melatonin receptors type 1B expression in aging cell cultures more strongly than HA‐S (developed for patients 35–65 years old). In age (stressed) cells, a lower expression of Klotho protein and melatonin receptors 1A and 1B is apparent. The addition of HA‐Y and HA‐S stimulates their expression thus providing a “protective” effect. The blue light irradiation at 40 J/cm2 performed in NHDF fibroblast cultures led to a modification of the expression of several genes, all involved in mechanisms known to be modulated in case of solar radiation stress. Conclusions Altho...

show abstract

Section: Methodsmentioning

confidence: 99%

“…Cells were prepared from human abdominoplasty skin micro-biopsies (abdominoplasty). 41 Ex vivo skin sections (2-5 mm 2 ) were incubated in 50 ml thermolysin for 3 h at 37°C. The epidermis was then separated from the Dermis using forceps.…”

Section: Cell Preparationmentioning

confidence: 99%

Effect of a hyaluronic acid‐based mesotherapeutic injectable on the gene expression of CLOCK and Klotho proteins, and environmentally induced oxidative stress in human skin cells

Prokopov¹,

Дробинцева

Kvetnoy

et al. 2022

J of Cosmetic Dermatology

View full text Add to dashboard Cite

show abstract

Immunohistochemical distribution of Ki67 in epidermis of thick glabrous skin of human digits

et al. 2017

View full text Add to dashboard Cite

The glabrous skin on the flexor sides of hands and feet, compared to other integument regions, has thicker epidermis and more complex pattern of epidermal ridges, wherefore in microscopy is denominated as thick skin. The epidermis of this skin type has individually unique and permanent superficial patterns, called dermatoglyphics, which are maintained by regenerative potential of deep epidermal rete ridges, that interdigitate with adjacent dermis. Using light microscopy, we analyzed cadaveric big toes thick skin samples, described histology of deep epidermal ridges (intermediate, limiting, and transverse), and quantitatively evidenced their pattern of proliferation by immunohistochemical assessment of Ki67. Immunohistochemical distribution of Ki67 was confined to basal and suprabasal layers, with pattern of distribution specific for intermediate, limiting and transverse ridges that gradually transform within epidermal height. Deep epidermal ridges, interdigitating with dermal papillae, participate in construction of intricate epidermal base, whose possible role in epidermal regeneration was also discussed. Having a prominent morphology, this type of epidermis offers the best morphological insight in complexities of skin organization, and its understanding could challenge and improve currently accepted models of epidermal organization.

show abstract

Dissociation, Quantification and Culture of Normal Human Merkel Cells Among Epidermal Cell Populations Derived from Glabrous and Hairy Skin Sites

Cited by 2 publications

References 15 publications

Effect of a hyaluronic acid‐based mesotherapeutic injectable on the gene expression of CLOCK and Klotho proteins, and environmentally induced oxidative stress in human skin cells

Effect of a hyaluronic acid‐based mesotherapeutic injectable on the gene expression of CLOCK and Klotho proteins, and environmentally induced oxidative stress in human skin cells

Immunohistochemical distribution of Ki67 in epidermis of thick glabrous skin of human digits

Contact Info

Product

Resources

About