Dissociation of Nuclear and Cytoplasmic Cell Cycle Progression by Drugs Employed in Cell Synchronization

An analysis of changes in the expression of cyclins A and B1 by the cell array system during the cell cycle: Comparison between cell synchronization methods

“…The level of cyclin B1 expression was different between the two methods as well as that of cyclin A. A striking increase in p53 and p21 proteins was found in chemically-synchronized cells (13), and the level of cyclins A and B1 was higher in HeLa cells synchronized by drugs than in normally growing controls (1,14,15). Therefore caution should be exercised when interpreting data from experiments using cells synchronized by chemical agents.…”

G1 arrest of U937 cells by onconase is associated with suppression of cyclin D3 expression, induction of p16INK4A, p21WAF1/CIP1 and p27KIP and decreased pRb phosphorylation

“…Synchronization of tumor-transformed cells, which requires use of the agents suppressing DNA replication, induces significant growth imbalance which leads to experimental bias, especially in analysis of the components of the cell cycle regulatory machinery. 37,38 Progression of U937 cells through the cell cycle was perturbed in the presence of onconase which manifested in an accumulation of cells in G 0/1 . The cytostatic effect occurred with a delay and significant accumulation of G 0/1 cells was seen only after 24 h of incubation with the drug.…”

Retinoic acid-mediated growth arrest of EBV-immortalized B lymphocytes is associated with multiple changes in G1 regulatory proteins: p27Kip1 up-regulation is a relevant early event

“…The stock solutions were stored at 7208C protected from light and oxygen. In some experiments, cells were synchronized at the G 1 /S boundary by aphidicolin block (with 5 mg/ml for 24 h) (Urbani et al, 1995).…”