Dissociation of doublecortin expression and neurogenesis in unipolar brush cells in the vestibulocerebellum and dorsal cochlear nucleus of the adult rat

Paolone, Nicholas A.; Manohar, Senthilvelan; Hayes, Sarah H.; Wong, Keit Men; Salvi, Richard; Baizer, Joan S.

doi:10.1016/j.neuroscience.2014.01.026

Cited by 11 publications

(7 citation statements)

References 55 publications

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“…S3 ). Although recent studies suggest that non-neurogenic cells with weak DCX-positivity are detectable in various brain regions 40 41 , we found that the number of strong DCX-positive cells was significantly elevated compared with that of PBS-treated controls. Additionally, these DCX-positive cells were only detectable in the lateral ventricles and hippocampal regions of stroke-damaged hemispheres at day 7, but were not detected in the cerebral cortex or other brain regions.…”

Section: Discussioncontrasting

confidence: 99%

Microvesicles from brain-extract—treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke

Lee

Kim

Choi

et al. 2016

Sci Rep

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Transplantation of mesenchymal stem cells (MSCs) was reported to improve functional outcomes in a rat model of ischemic stroke, and subsequent studies suggest that MSC-derived microvesicles (MVs) can replace the beneficial effects of MSCs. Here, we evaluated three different MSC-derived MVs, including MVs from untreated MSCs (MSC-MVs), MVs from MSCs treated with normal rat brain extract (NBE-MSC-MVs), and MVs from MSCs treated with stroke-injured rat brain extract (SBE-MSC-MVs), and tested their effects on ischemic brain injury induced by permanent middle cerebral artery occlusion (pMCAO) in rats. NBE-MSC-MVs and SBE-MSC-MVs had significantly greater efficacy than MSC-MVs for ameliorating ischemic brain injury with improved functional recovery. We found similar profiles of key signalling proteins in NBE-MSC-MVs and SBE-MSC-MVs, which account for their similar therapeutic efficacies. Immunohistochemical analyses suggest that brain-extract—treated MSC-MVs reduce inflammation, enhance angiogenesis, and increase endogenous neurogenesis in the rat brain. We performed mass spectrometry proteomic analyses and found that the total proteomes of brain-extract—treated MSC-MVs are highly enriched for known vesicular proteins. Notably, MSC-MV proteins upregulated by brain extracts tend to be modular for tissue repair pathways. We suggest that MSC-MV proteins stimulated by the brain microenvironment are paracrine effectors that enhance MSC therapy for stroke injury.

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Section: Discussioncontrasting

confidence: 99%

Microvesicles from brain-extract—treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke

Lee

Kim

Choi

et al. 2016

Sci Rep

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“…We used sections from the brains of three rats (2–4 months) that were prepared in the context of other projects (Manohar et al, ; Paolone et al, ). Those animals had been perfused with 4% PFA, the brains postfixed overnight and cryoprotected in 15% then 30% sucrose in PBS.…”

Section: Methodsmentioning

confidence: 99%

Laminar and Neurochemical Organization of the Dorsal Cochlear Nucleus of the Human, Monkey, Cat, and Rodents

Baizer

Wong

Paolone

et al. 2014

The Anatomical Record

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The dorsal cochlear nucleus (DCN) is a brainstem structure that receives input from the auditory nerve. Many studies in a diversity of species have shown that the DCN has a laminar organization and identifiable neuron types with predictable synaptic relations to each other. In contrast, studies on the human DCN have found a less distinct laminar organization and fewer cell types, although there has been disagreement among studies in how to characterize laminar organization and which of the cell types identified in other animals are also present in humans. We have reexamined DCN organization in the human using immunohistochemistry to analyze the expression of several proteins that have been useful in delineating the neurochemical organization of other brainstem structures in humans: nonphosphorylated neurofilament protein (NPNFP), nitric oxide synthase (nNOS), and three calcium-binding proteins. The results for humans suggest a laminar organization with only two layers, and the presence of large projection neurons that are enriched in NPNFP. We did not observe evidence in humans of the inhibitory interneurons that have been described in the cat and rodent DCN. To compare humans and other animals directly we used immunohistochemistry to examine the DCN in the macaque monkey, the cat, and three rodents. We found similarities between macaque monkey and human in the expression of NPNFP and nNOS, and unexpected differences among species in the patterns of expression of the calcium-binding proteins.

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“…Loss of the neurofibromatosis type 1 ( Nf1 ) gene leads to imbalance in generating the appropriate numbers of glial cells, GC/UBC fate-specification/differentiation and GC/UBC migration (Kim et al, 2014). Studies have also shown a role for DCX in the neurogenesis and migration of UBCs (Manohar et al, 2012; Paolone et al, 2014). Furthermore, Wnt1 glycoprotein expression in the upper RL and UBCs is related to molecular identity and cell migration in cerebellar development (Hagan and Zervas, 2012).…”

Section: The Neuronal Migration In the Developing Cerebellummentioning

confidence: 99%

Neuronal Migration During Development of the Cerebellum

Rahimi-Balaei

Bergen

Kong

et al. 2018

Front. Cell. Neurosci.

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Neuronal migration is a fundamental process in central nervous system (CNS) development. The assembly of functioning neuronal circuits relies on neuronal migration occurring in the appropriate spatio-temporal pattern. A defect in the neuronal migration may result in a neurological disorder. The cerebellum, as a part of the CNS, plays a pivotal role in motor coordination and non-motor functions such as emotion, cognition and language. The excitatory and inhibitory neurons within the cerebellum originate from different distinct germinal zones and migrate through complex routes to assemble in a well-defined neuronal organization in the cerebellar cortex and nuclei. In this review article, the neuronal migration modes and pathways from germinal zones to the final position in the cerebellar cortex and nuclei will be described. The cellular and molecular mechanisms involved in cerebellar neuronal migration during development will also be reviewed. Finally, some diseases and animal models associated with defects in neuronal migration will be presented.

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Dissociation of doublecortin expression and neurogenesis in unipolar brush cells in the vestibulocerebellum and dorsal cochlear nucleus of the adult rat

Cited by 11 publications

References 55 publications

Microvesicles from brain-extract—treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke

Microvesicles from brain-extract—treated mesenchymal stem cells improve neurological functions in a rat model of ischemic stroke

Laminar and Neurochemical Organization of the Dorsal Cochlear Nucleus of the Human, Monkey, Cat, and Rodents

Neuronal Migration During Development of the Cerebellum

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