Dissociation Between the Content of Vasopressin and Neurosecretory Material in the Rat Neurohypophysis

Moses, AM; Leveque, Theodore F.; Giambattista, Mary; Lloyd, Charles W.

doi:10.1677/joe.0.0260273

Cited by 24 publications

(3 citation statements)

References 14 publications

(19 reference statements)

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“…Scharrer (1952) and Leveque & Scharrer (1953) found that dehydration caused a depletion of the neurosecretory material in the neurohypophysis, that rehydration led to a progressive re-accumulation of this secretory material in the pars neuralis and that an approximately normal amount was noted in animals killed 1 week after rehydration. As reported by Moses, Leveque, Giambattista & Lloyd (1963), however, a correlation between the amount of neurohypophysial hormones and the neurosecretory material in the neurohypophysis was not demon¬ strated in rats subjected to painful stimuli. Therefore, a quantitative study of the repletion of vasopressin and oxytocin in the rat neural lobe was undertaken.…”

Section: Introductionmentioning

confidence: 55%

“…Although Scharrer (1952), in his morphological study, found that it took only 1 week for the depleted neural lobe to resume its normal appearance in respect of neurosecretory material, the stainable material probably is distinct from neuro¬ hypophysial hormones as suggested by the results of Moses et al (1963). Moreover, Daniel & Lederis (1966), who studied electron-dense particles in neurohypophysial nerve fibres of the rat in respect of effects of ether anaesthesia and haemorrhage, have suggested that the quantity of electron-dense material is not related to hormone content.…”

Section: Discussionmentioning

confidence: 98%

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Repletion of Vasopressin and Oxytocin in the Posterior Lobe of the Pituitary Gland of the Rat

Young¹,

Dyke²

1968

Journal of Endocrinology

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Rats deprived of drinking water for 7 days showed a striking depletion of neurohypophysial hormones from the posterior lobe of the pituitary gland. The average daily depletion rate was estimated to be 93 m-u. for vasopressin and 97 m-u. for oxytocin. When rats were allowed free access to water, dehydration was rapidly corrected as shown by normal haematocrit values and plasma osmolarities. Repletion of neurohypophysial hormones, rapid in the first 24 hr., continued gradually thereafter. The mean calculated repletion rate was 41 m-u./day for vasopressin and 42 m-u./day for oxytocin. Repletion was completed about 14 days after rehydration.

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Section: Introductionmentioning

confidence: 55%

Section: Discussionmentioning

confidence: 98%

Repletion of Vasopressin and Oxytocin in the Posterior Lobe of the Pituitary Gland of the Rat

Young¹,

Dyke²

1968

Journal of Endocrinology

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“…Based on these reports in nursing as well as in parturient animals, we consider that the disappearance of stainable and osmiophilic material during suckling may reflect an intragranular or axoplasmal reorganization of neuro¬ secretory material (hormone-neurophysin complex) and may be due to changed stainability rather than to a real discharge of this material into the blood stream. Further, the discrepancy between the neural lobe content of hormones and stainable neurosecretory material has previously been pointed out by several authors (Drager & Rennels, 1955; Moses, Leveque, Giambatista & Lloyd, 1963;Daniel & Lederis, 1966). In rats subjected to acute bleeding or strong osmotic stimuli a reduction in radioactivity offraction A was observed (Norström & Sjöstrand, 1971c, 1972.…”

Section: Seriesmentioning

confidence: 93%

Effect of Suckling and Parturition on Axonal Transport and Turnover of Neurohypophysial Proteins of the Rat

Norström¹,

Sjöstrand²

1972

Journal of Endocrinology

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The effect of suckling and parturition on axonal transport and turnover of neurohypophysial proteins was studied by determining the radioactivity in neural lobe proteins after injection of [35S]cysteine into the region of the supraoptic and paraventricular nuclei in rats.Evidence was obtained for a lag period of more than 1 h before newly synthesized material is ready for transport along the neurohypophysial tract. Neither suckling nor parturition induced any noticeable reduction in amount of neural lobe neurophysin. The axonal transport of newly synthesized neurophysin was increased after suckling and parturition. Gel electrophoresis showed the same pattern of radiolabelling of neurohypophysial proteins as in normal female rats. The turnover of neurohypophysial proteins was increased after prolonged suckling. Differences in mode of release of oxytocin-and vasopressin-neurosecretory material are discussed. It is suggested that the neurohypophysial pool of oxytocic neurosecretory products is homogeneous.

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Early secretory activity in the hypothalamic nuclei and neurohypophysis in the rat; determined by selective staining

Dawson

1966

Journal of Morphology

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Visualization of stainable material in the neural lobe of the rat provided the most reliable index of the age at which secretory activity can first be recognized, though preceded by both hypothalamic synthesis and axonal transportation. A problem of interpretation was encountered in the neural lobes of fetal an3 infant animals, due to different staining responses obtained during this age period, to the two methods of staining employed; chrome alum hematoxylin-phloxin and aldehyde fuchsin after oxidation by either acidified potassium permanganate or performic acid. With aldehyde fuchsin the material of the neural lobe is stainable selectively from the eighteenth day of fetal life to adulthood. With hematoxylin phloxine the first staining response also occurred in the posterior lobe but much later, at the end of the first postnatal week. The staining situation i n the pars neuralis has its counterpart in the differentiating hypothalamic nuclei; complicated by the differentiation of the supraoptic nuclei some days in advance of the paraventricular nuclei. After aldehyde fuchsin staining, evidences of neurosecretory activity were present in the perikarya of the supraoptic nuclei at birth, but mature neurons were rarely seen in the paraventricularis until at least 24 hours later. Nuclei of fetal hypothalami were not studied, but the demonstration of stainable material in the fetal neural lobes constitutes circumstantial evidence of functional competence of some neurons of either one or both types of nucleus, most likely the supraoptic.Some time ago (Dawson, '53) a brief record was made of evidences of early neurosecretory activity in the hypothalamoneurohypophyseal neurosecretory system of the rat, shortly after a comparable study had been reported by Green, Catalan0 and Van Breemen ('53), with a more extended account in 1955 (Green and Van Breeman). There was some disparity in our findings probaly due to reliance on different methods of selective staining of neurosecretory material. These authors and many others who subsequently sought to demonstrate neurosecretory material in newborn and infantile rats used only Gomori's chrome alum hematoxylin phloxine while I used, in addition, Halmi's ('52) aldehyde fuchsin method and variants thereof, especially in the kind and degree of oxidation before staining.These early observations based on histological methods have been supplemented by other studies on the occurrence and amount of neurohypophyseal hormones present in the neural lobes of fetal, newborn, infantile and adult rats, as deter-J. MORPH., 118: 549-564.mined by extraction and the use of standard assays. At the present time there are sufficient quantitative data to permit a limited correlation to be made between density of staining and the number of units of extractable hormone.The hypothalamo-hypophyseal system of the rat probably does not provide optimal material for a study of this type which seeks to gain evidence of early functional activity by determining the time, and place of appearance, and quanti...

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Dissociation Between the Content of Vasopressin and Neurosecretory Material in the Rat Neurohypophysis

Cited by 24 publications

References 14 publications

Repletion of Vasopressin and Oxytocin in the Posterior Lobe of the Pituitary Gland of the Rat

Repletion of Vasopressin and Oxytocin in the Posterior Lobe of the Pituitary Gland of the Rat

Effect of Suckling and Parturition on Axonal Transport and Turnover of Neurohypophysial Proteins of the Rat

Early secretory activity in the hypothalamic nuclei and neurohypophysis in the rat; determined by selective staining

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