Dissimilatory iron and sulfate reduction by native microbial communities using lactate and citrate as carbon sources and electron donors

Xia, Di; Yi, Xiaoyun; Lu, Yang; Huang, Weilin; Xie, Yingying; Han, Ye; Dang, Zhi; Tao, Xueqin; Li, Li; Lü, Guang-Hong

doi:10.1016/j.ecoenv.2019.03.005

Cited by 52 publications

(18 citation statements)

References 52 publications

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“…Queiroz et al (2018) reported a significant correlation between Fe, Pb and other trace metals in tailing deposits after the initial impacts in the Rio Doce estuary in 2015. The iron oxides from tailings deposited in the estuary have a strong capacity of metal retention (Cornell and Schwertmann 2003;Yin et al 2016); and they are likely to be released due dissimilatory iron reduction under estuarine conditions (Bonneville et al 2009;Queiroz et al, 2018;Xia et al 2019). Although anoxic estuarine soils favor the formation of sulfides (e.g.…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Chronic trace metals effects of mine tailings on estuarine assemblages revealed by environmental DNA (v0.1)"

2019

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Mine tailing disasters have occurred worldwide and contemporary release of tailings of large proportions raise concerns of the chronic impacts that trace metals may have on the aquatic biodiversity. Environmental metabarcoding (eDNA) offers an yet poorly explored opportunity for biological monitoring of impacted aquatic ecosystems from mine tailings and contaminated sediments. eDNA has been increasingly recognized to be an effective method to detect previously unrecognized small-sized Metazoan taxa, but their ecological responses to environmental pollution has not been assessed by metabarcoding. Here we evaluated chronic effects of trace metal contamination from sediment eDNA of the Rio Doce estuary, 1.7 years after the Samarco mine tailing disaster, which released over 40 million m 3 of iron tailings in the Rio Doce river basin. We identified 123 new sequence variants (eOTUs) of benthic taxa and an assemblage composition dominated by Nematoda, Crustacea and Platyhelminthes; typical of other estuarine ecosystems. We detected environmental filtering on the meiofaunal assemblages and multivariate analysis revealed strong influence of Fe contamination, supporting chronic impacts from mine tailing deposition in the estuary. This was in contrast to environmental filtering of meiofaunal assemblages of non-polluted estuaries. Here we suggest that the eDNA metabarcoding technique provides an opportunity to fill up biodiversity gaps in coastal marine ecology and may become a valid method for long term monitoring studies in mine tailing disasters and estuarine ecosystems with high trace metals content.

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Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Chronic trace metals effects of mine tailings on estuarine assemblages revealed by environmental DNA (v0.1)"

2019

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“…By acid dissolution of the precipitate in the effluent, Fe(II) and Fe(III) were found in the precipitate and the content of Fe(III) was less. It was proved that the precipitate was mainly FeS with a small amount of ferric hydroxide precipitate [26,27,39]. According to the analysis of sulfide concentration change at this stage, it is inferred that the main process is the oxidation-reduction reaction between Fe(III) and sulfide to form Fe(II) and elemental sulfur, accompanied by the formation of a small amount of FeS precipitation.…”

Section: Conversion Analysis Of Fe Elementsmentioning

confidence: 93%

“…Chen et al found that many sulfides produced by sulfate reduction existed in the form of hydrogen sulfide in simultaneous removal of SO 2 and NO in a rotating drum biofilter [9,10]. Both Zhou et al [26] and Xia et al [27] observed the formation of FeS in the sulfate reduction system containing ferric iron. When the sulfate concentration was increased to 25 mM, the sulfate removal efficiency was more affected and would take a long time to recover to more than 90%.…”

Section: Simultaneous Biological and Chemical Removal Of Sulfate And mentioning

confidence: 99%

Simultaneous Biological and Chemical Removal of Sulfate and Fe(II)EDTA-NO in Anaerobic Conditions and Regulation of Sulfate Reduction Products

Sun

2019

Minerals

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In the simultaneous flue gas desulfurization and denitrification by biological combined with chelating absorption technology, SO 2 and NO are converted into sulfate and Fe(II)EDTA-NO which need to be reduced in biological reactor. Increasing the removal loads of sulfate and Fe(II)EDTA-NO and converting sulfate to elemental sulfur will benefit the application of this process. A moving-bed biofilm reactor was adopted for sulfate and Fe(II)EDTA-NO biological reduction. The removal efficiencies of the sulfate and Fe(II)EDTA-NO were 96% and 92% with the influent loads of 2.88 kg SO 4 2− ·m −3 ·d −1 and 0.48 kg NO·m −3 ·d −1 . The sulfide produced by sulfate reduction could be reduced by increasing the concentrations of Fe(II)EDTA-NO and Fe(III)EDTA. The main reduction products of sulfate and Fe(II)EDTA-NO were elemental sulfur and N 2 . It was found that the dominant strain of sulfate reducing bacteria in the system was Desulfomicrobium. Pseudomonas, Sulfurovum and Arcobacter were involved in the reduction of Fe(II)EDTA-NO.

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“…This indicates that the source of the sulfate in the deployed Fe(III)-coated pumice slurries is most likely from the spring water well. Oxoanions such as sulfate can potentially be adsorbed and accumulated onto the surface of Fe(III) minerals (Hinkle et al, 2015) and could co-exist to enhance sequential Fe(III)-sulfate reduction (Xia et al, 2019). This may explain why the Fe(III)-coating (dominated by akaganeite) on the pumice surface facilitated the sorption of sulfate from spring water (contributing to sulfate concentrations up to ∼16 mg/L).…”

Section: Geochemical Changes During the Incubation Of The Deployed "Mmentioning

confidence: 99%

A Novel “Microbial Bait” Technique for Capturing Fe(III)-Reducing Bacteria

et al. 2020

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Microbial reduction of Fe(III) is a key geochemical process in anoxic environments, controlling the degradation of organics and the mobility of metals and radionuclides. To further understand these processes, it is vital to develop a reliable means of capturing Fe(III)-reducing microorganisms from the field for analysis and lab-based investigations. In this study, a novel method of capturing Fe(III)-reducing bacteria using Fe(III)-coated pumice "microbe-baits" was demonstrated. The methodology involved the coating of pumice (approximately diameter 4 to 6 mm) with a bioavailable Fe(III) mineral (akaganeite), and was verified by deployment into a freshwater spring for 2 months. On retrieval, the coated pumice baits were incubated in a series of lab-based microcosms, amended with and without electron donors (lactate and acetate), and incubated at 20 • C for 8 weeks. 16S rRNA gene sequencing using the Illumina MiSeq platform showed that the Fe(III)-coated pumice baits, when incubated in the presence of lactate and acetate, enriched for Deltaproteobacteria (relative abundance of 52% of the sequences detected corresponded to Geobacter species and 24% to Desulfovibrio species). In the absence of added electron donors, Betaproteobacteria were the most abundant class detected, most heavily represented by a close relative to Rhodoferax ferrireducens (15% of species detected), that most likely used organic matter sequestered from the spring waters to support Fe(III) reduction. In addition, TEM-EDS analysis of the Fe(III)-coated pumice slurries amended with electron donors revealed that a biogenic Fe(II) mineral, magnetite, was formed at the end of the incubation period. These results demonstrate that Fe(III)-coated pumice "microbe baits" can potentially help target metal-reducing bacteria for culture-dependent studies, to further our understanding of the nano-scale microbe-mineral interactions in aquifers.

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Dissimilatory iron and sulfate reduction by native microbial communities using lactate and citrate as carbon sources and electron donors

Cited by 52 publications

References 52 publications

Peer Review #1 of "Chronic trace metals effects of mine tailings on estuarine assemblages revealed by environmental DNA (v0.1)"

Peer Review #1 of "Chronic trace metals effects of mine tailings on estuarine assemblages revealed by environmental DNA (v0.1)"

Simultaneous Biological and Chemical Removal of Sulfate and Fe(II)EDTA-NO in Anaerobic Conditions and Regulation of Sulfate Reduction Products

A Novel “Microbial Bait” Technique for Capturing Fe(III)-Reducing Bacteria

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