Dissimilar microRNA-21 functions and targets in trophoblastic cell lines of different origin

Chaiwangyen, Wittaya; Ospina‐Prieto, Stephanie; Photini, Stella M.; Schleußner, E; Markert, Udo R.; Morales-Prieto, Diana M.

doi:10.1016/j.biocel.2015.08.018

Cited by 33 publications

(15 citation statements)

References 42 publications

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“…MiR-21 is a widely known oncomiR, with the enrichment in human placentas. Inhibited miR-21 reduced the cellular abilities of invasion, migration and proliferation in trophoblast cell lines JEG-3 and HTR-8/SVneo as well as inducing apoptosis in JEG-3 cells (Chaiwangyen et al, 2015). In our experiment, GAS5 and miR-21 were negatively regulated in the two trophoblast cell lines, and co-transfection of GAS5 and miR-21 could rescue the GAS5 expression.…”

Section: Discussionsupporting

confidence: 46%

Long Non-coding RNA Gas5 Is Associated With Preeclampsia and Regulates Biological Behaviors of Trophoblast via MicroRNA-21

Zheng

Hou

et al. 2020

Front. Genet.

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Preeclampsia is a lethal pregnancy specific hypertensive disorder involving multisystem. Despite extensive studies to investigate the causes of preeclampsia, the pathogenesis still remains largely unknown. Long non-coding RNAs (lncRNAs) are a diverse class of non-translated RNAs which play a crucial part in various biological phenomena. Although lncRNA Growth Arrest-Specific 5 (GAS5) aberrantly expressed in multiple cancer tissues and is implicated in multiple biological processes of tumor cells, little is known about its role in preeclampsia. In this study, 40 patients with preeclampsia and 32 gestational age matched normotension pregnant women were recruited. Using quantitative real-time polymerase chain reaction (qRT-PCR), we found higher expression of GAS5 in placenta of preclamsia affected women. The level of GAS5 existed strongly in correlation with Thrombin Time indicating coagulation function and other clinical parameters by Pearson correlation analysis. Then we constructed the GAS5 lentivirus expression vectors and transfected into human trophoblast cell lines HTR-8/SVneo and JEG-3. Using in vitro cell culture studies, we found an inhibited effect of GAS5 on proliferative ability, migratory ability and invasive ability however; no effect on apoptosis was detected. Further mechanistic analysis found that GAS5 modulated microRNA-21 (miR-21) in an opposite variation tendency by qRT-PCR and rescue experiment. In addition, inhibition of GAS5 promoted the activation of PI3K/AKT signaling pathway and its downstream proteins covering MMP-9 and TP53 as evident from our qRT-PCR and western blot analyses. Thus, we suggested that GAS5 might involve in pregnancy with preeclampsia by influencing the biological functions of trophoblast cells through the regulation of miR-21 and activation of PI3K/AKT signaling pathway and its downstream targets, which may contribute to reveal the nature of preeclampsia.

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Section: Discussionsupporting

confidence: 46%

Long Non-coding RNA Gas5 Is Associated With Preeclampsia and Regulates Biological Behaviors of Trophoblast via MicroRNA-21

Zheng

Hou

et al. 2020

Front. Genet.

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“…Milk miR-21, a signature miR of commercial dairy milk and cargo of bovine milk exosomes [71,72,80], most likely reaches the placenta and increases placental and trophoblast growth. miR-21 plays important roles in growth of trophoblastic cell lines [101] and increases PI3K-AKTand mTORC1 signaling by suppression of various key inhibitory checkpoints such as PTEN [11]. Stimulation of trophoblast mTORC1 activity enhances the transfer of branched-chain amino acids (BCAAs) to the fetus, a constellation that may promote BCAA-mTORC1-driven fetal macrosomia [102][103][104][105][106][107] (Fig.…”

Section: Fetal Macrosomiamentioning

confidence: 99%

Exosomes of pasteurized milk: potential pathogens of Western diseases

Melnik

Schmitz

2019

J Transl Med

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Milk consumption is a hallmark of western diet. According to common believes, milk consumption has beneficial effects for human health. Pasteurization of cow's milk protects thermolabile vitamins and other organic compounds including bioactive and bioavailable exosomes and extracellular vesicles in the range of 40-120 nm, which are pivotal mediators of cell communication via systemic transfer of specific micro-ribonucleic acids, mRNAs and regulatory proteins such as transforming growth factor-β. There is compelling evidence that human and bovine milk exosomes play a crucial role for adequate metabolic and immunological programming of the newborn infant at the beginning of extrauterine life. Milk exosomes assist in executing an anabolic, growth-promoting and immunological program confined to the postnatal period in all mammals. However, epidemiological and translational evidence presented in this review indicates that continuous exposure of humans to exosomes of pasteurized milk may confer a substantial risk for the development of chronic diseases of civilization including obesity, type 2 diabetes mellitus, osteoporosis, common cancers (prostate, breast, liver, B-cells) as well as Parkinson's disease. Exosomes of pasteurized milk may represent new pathogens that should not reach the human food chain.

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“…Concordantly, in T. cruzi -infected mice, increased miR-21 expression in the heart has been correlated with a parasitemia peak at 30 days post-infection ( 39 ). In placenta, miR-21 has been associated with trophoblast differentiation and invasion and miR-21 dysregulation leads to placental pathology ( 45 ). MiR-146a is a negative feedback regulator in TLR-4 signaling that acts by repressing TRAF6 to inhibit NFκB transcription factor activation ( 46 , 47 ).…”

Section: Discussionmentioning

confidence: 99%

Trypanosoma cruzi and Toxoplasma gondii Induce a Differential MicroRNA Profile in Human Placental Explants

et al. 2020

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Trypanosoma cruzi and Toxoplasma gondii are two parasites than can be transmitted from mother to child through the placenta. However, congenital transmission rates are low for T. cruzi and high for T. gondii. Infection success or failure depends on complex parasite-host interactions in which parasites can alter host gene expression by modulating non-coding RNAs such as miRNAs. As of yet, there are no reports on altered miRNA expression in placental tissue in response to either parasite. Therefore, we infected human placental explants ex vivo by cultivation with either T. cruzi or T. gondii for 2 h. We then analyzed the miRNA expression profiles of both types of infected tissue by miRNA sequencing and quantitative PCR, sequence-based miRNA target prediction, pathway functional enrichment, and upstream regulator analysis of differentially expressed genes targeted by differentially expressed miRNAs. Both parasites induced specific miRNA profiles. GO analysis revealed that the in silico predicted targets of the differentially expressed miRNAs regulated different cellular processes involved in development and immunity, and most of the identified KEGG pathways were related to chronic diseases and infection. Considering that the differentially expressed miRNAs identified here modulated crucial host cellular targets that participate in determining the success of infection, these miRNAs might explain the differing congenital transmission rates between the two parasites. Molecules of the different pathways that are regulated by miRNAs and modulated during infection, as well as the miRNAs themselves, may be potential targets for the therapeutic control of either congenital Chagas disease or toxoplasmosis.

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Dissimilar microRNA-21 functions and targets in trophoblastic cell lines of different origin

Cited by 33 publications

References 42 publications

Long Non-coding RNA Gas5 Is Associated With Preeclampsia and Regulates Biological Behaviors of Trophoblast via MicroRNA-21

Long Non-coding RNA Gas5 Is Associated With Preeclampsia and Regulates Biological Behaviors of Trophoblast via MicroRNA-21

Exosomes of pasteurized milk: potential pathogens of Western diseases

Trypanosoma cruzi and Toxoplasma gondii Induce a Differential MicroRNA Profile in Human Placental Explants

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